Next Advance - Innovative Lab Products for the Life Sciences
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5/10/2013 GO Lab homogenizes beetle legs
The GoLab homogenized beetle legs in the Bullet Blender Storm for the Biology Department of Loyola University last month, saving countle ...
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Next Advance, Inc.
1548 Burden Lake Road
Averill Park, NY 12018, USA
Phone: 1.518.674.3510
in USA 1.800.738.1681
Fax: 1.518.674.0189
info@nextadvance.com
Throw out your timers! Don't babysit your samples again!
Automate your processing / washing of Western, Northern, and Southern blots and gels. Ideal for unattended staining of gels.
NEW! The Freedom Rocker™ BlotBot™
Introducing the best-valued blot processor on the market! We took the features you love from previous models and added improvements to bring you the easiest and most convenient automated blot or gel processor you'll ever experience.
- Dispense as little as 3 mL of reagent into each tray and recover separately- perfect for precious primary antibodies
- Holds 1 midi blot or 2 mini blots or strips
- LCD display allows for easy programming of custom protocols
- Fully customizable programs have been preloaded for convenience
- Save hundreds of dollars
Available for Pre-Order
Questions about this product? Contact us at info@nextadvance.com
It's a complete, fully programmable blot processing station!
The Freedom Rocker™ is an automated blot washer. It is a very quiet laboratory platform rocker with a pump and a microprocessor for precise timing and user programming of protocols. It automatically processes blots and gels. It frees you from babysitting your samples by relieving you from the frequent pouring of liquids to and from trays.
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"I'm one of the first users of the Freedom Rocker at Amgen in Cambridge, MA. The machine gets LOTS of use from all of us here."
Dr. Barbara Grubinska, Amgen
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"We love it! It saves us a lot of time."
Dr. Lilliane Roellard, Genentech
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"It is perfect for silver staining of gels for mass spectrometry. It has made an UNBELIEVABLE impact on data generation for me in cancer protein screening."
Dr. Steve Griffiths, Atlantic Cancer Research Institute
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"We are very pleased with the instrument."
Dr. Richard Ingram, Schering-Plough
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"The Freedom Rocker is really convenient. I use it for washing gels while I work on other things, and also as a regular rocker."
Dr. Michele Lastro, The UAlbany Gen*NY*Sis Center for Excellence in Cancer Genomics
Are you chained to your timer? Throw it out and use the FREEDOM ROCKER™. Simply place your blots or gels or slides in trays, set the program, connect the bottles/tubes of reagents and walk away. The microcontroller in the FREEDOM ROCKER™ will ensure that the reagents and washes will be accurately pumped into and out of the trays at the precise times. Results will be consistent because all trays will receive the same reagent or wash buffer at the same time in equal volumes. Multi-dispense models can even dispense one different reagent to each tray, i.e. primary or secondary antibody.
Be productive and concentrate on another task, enjoy your lunch, OR let it run overnight!
Improve Consistency and Accuracy
By automating the processing of blots and gels, the Freedom Rocker™ yields very consistent and accurate blots, gels, and slides. The Freedom Rocker™ automatically dispenses and aspirates equal volumes of reagents from multiple trays simultaneously. The samples in all the trays will experience the exact same protocol. Back to top
Increase Productivity
The Freedom Rocker™ automates the processing of blots and gels,. What would you do if you did not have to babysit your blots? Through increased productivity, the Freedom Rocker™ pays for itself in about 30 uses. Back to top
Get Results Sooner
Start processing a blot before you leave today and have it ready for you tomorrow morning. The Freedom Rocker™ allows you to process blots and gels anytime... even during meetings or overnight. No need to wait until the next morning to start processing. Back to top
Risk Free Purchase
The Freedom Rocker™ comes with a 30 day money back guarantee and a 2 year warranty. Back to top
The FREEDOM ROCKER™ enables you to be productive while processing gels, membranes, or slides.
Portion of a sample protocol - showing the addition and washing of one reagent using the Freedom Rocker. The Freedom Rocker can be programmed to perform up to 4 of these reagent steps, and process your Western blots and gels unattended. Your blots can be ready to analyze when you arrive at work the following morning.
Western blot analysis of extracts from human squamous carcinoma cells using anti-myc antibody. Courtesy of Dr. Aparna Ranganathan, the University at Albany (SUNY).
The FREEDOM ROCKER™ can run in manual mode to perform a dispense, timed rocking, or aspiration step, or in automatic mode where it can automatically perform up to 9 protocol steps. Each step can be a reaction with a reagent or a series of washes (up to 5 repetitions) such as 5X dispense, rock, and aspirate. For each step, the instrument can select among 3 buffers/reagents and aspirate to waste or either of 2 recovery containers, and clearing rinses can be included for more efficient washing.
The multi-dispense model can dispense a different antibody into each tray.
With this option, antibodies loaded into each tube will be dispensed into the corresponding tray at the programmed time.
Freedom Rocker starter kits include bottles and trays. See the pricelist for further details.
(Prices do not include shipping or taxes)
DX2 |
DX2-MD |
DX4 |
DX4-MD |
|
|
Maximum number of trays |
2 |
2 |
4 |
4 |
|
Multi-Dispense Feature? |
No |
Yes |
No |
Yes |
Features
- Unattended dispensing and aspiration of liquids to 1 or 2 trays in the FRSKDX2 model, or 1, 2, or 4 trays in the FRSKDX4 model.
- Compact. Uses only 12 in. of lab bench space
- Only requires an electrical outlet - no vacuum, pressurized air or gas, or additional pumps required.
- Quiet.
- Can recover reagent solution.
- Drip proof quick-release connections.
- Adjustable Parameters for each protocol step include:
- rocking speed (0 to 40 cycles per minute)
- reagent/solution rocking time (1 min to 90 hours)
- dispense volume (10 to 600 ml)
- up to 5 repetitions per step.
- clearing rinse option
- Patent pending technology.
- Rocking continues after the final rinse and the wash buffer remains in the trays to prevent the blots or gels from dessicating.
- Customization available (e.q. aspirate into particular bottles or carboys, custom platform sizes).
- Built in the USA for years of trouble free use, including: a stainless steel platform and front panel; a powder coated heavy-duty gage aluminum chassis; self-lubricating bronze bushings; and a Pittman gear motor driving the rocking motion.
- Uses existing protocols. Programming is easy and follows standard protocols steps. Can program up to 9 steps where each step is an immersion in a reagent (e.g. antibody) or a series of washes.
- Each step - Select dispense from any of 3 reagents/buffers. Reagents will be dispensed and aspirated to all the trays simultaneously, for consistent processing of all the blots and gels (one reagent at a time will go to all the trays). Select aspirate to waste or either of 2 recovery containers. Minimum dispense volume is 10 mL per tray.
- Clearing rinse can be activated or deactivated for each step individually.
- Alarm at the end of the last step can be activated or deactivated.
- Color coded tubing simplify connections to bottles.
- Warranty: 2 years parts and labor.
- 30 day money back guarantee.
Multi-Dispense Features
- Can dispense different reagents to each tray.
- Reagents are dispensed from syringes on the dispense head.
- Multi-dispensed reagents cannot be recovered sperately.
Connections
- 3 dispense bottles
- 3 collection bottles
- Connect to any bottle or 50ml tube with One>Click™ caps
- Color-coded tubing prevents errors in connection.
- Recovers reagent solution
- Internal pump included - Connection to an external pump, vacuum, or pressurized air is NOT required.
Trays
- Size ranges from 125 ml (6 cm x 4.5 cm) to 1700 ml (22 cm x 16 cm)
- Trough along the perimeter for improved reagent recovery
One>Click™ caps
- leak-proof
- rapid tube attachment and removal
- various sizes to fit 50ml tubes, 100ml and larger Pyrex® bottles, Nalgene bottles 1L and larger, and Nalgene carboys
Warranty
- 30-day money-back guarantee
- two year warranty
Specifications
Can the FREEDOM ROCKER™ work in labs in other countries?
Yes. A wall transformer will convert the voltage from your wall outlet to 12V DC for the Freedom Rocker™. Select the proper voltage when you order your instrument.
How much room does the FREEDOM ROCKER™ require?
The Freedom Rocker™ takes up only 10 inches (25 cm) of lab bench. It is 10 inches (25 cm) wide by 15 inches (38 cm) deep, including the pump and the rocker. The bottles for wash buffer, waste, and reagent collection can be placed behind or to the side of the Freedom Rocker™, or even on a shelf above or below the lab bench.
Can the Freedom Rocker™ drain the waste to a sink?
Yes, the hose for waste liquid can either run to a container or to a sink.
What happens at the end of the final wash step?
After the programmed duration of the final wash step, the instrument beeps to let the user know that the wash is complete. However, to keep the membranes, gels, or slides from drying out, the rocking continues and the wash buffer is not pumped from the tray(s).
How many trays can the FREEDOM ROCKER™ run at once?
The Freedom Rocker™ model FRSKDX2 can run 1 large tray or 2 tray small or medium trays. With the purchase of AHDS24 Small Aspiration Head, one small or medim tray can be used. The FRSKDX4 model runs 4 trays or 2 trays at once. To run just 1 tray at a time, a different aspiration head is required. Aspiration heads and different tube sets can be ordered separately. See the pricelist for ordering information and current pricing.
Do all the trays follow the same protocol?
Yes, the Freedom Rocker™ pumps the liquids in and out of all the trays simultaneously to/from the same reagent/waste containers. Different reagents can be manually poured in the different trays initially, or automatically with the multi-dispense option, however, when these reagents are aspirated out of the trays, they will be combined in the waste or recover bottle.
Can I dispense different antibodies into each tray?
Yes, with the Multi-dispense (MD) models. Individual antibodies are loaded into the syringes on the dispense head. Antibodies cannot be individually recovered.
Operation
How do I switch from 4 to 2 trays?
The DX4 model can use 1, 2 or 4 sample trays. To switch between 2 and 4 trays, on newer models, simply move the plunger in the manifold of the tube set. In older tube sets, you may have to swap out the tube set.
How do I use just a single tray?
To use just a single sample tray, set up the Freedom Rocker just as you would for using 2 trays. Then attach the small dispense head AHD24. Since the instrument is now dispensing and aspirating into a single tray, twice as much buffer or reagent will be pumped into or out of the single tray, so set your volumes to half the desired amounts.
How do I dispense as little antibody as possible?
To dispense 10 ml or less per tray, attach the 50 mL tube to the blue tubing and set the fill volume to 30 mL and the clearing rinse to on. The Freedom Rocker can only dispense as much antibody as is in the tube, so if the 50 mL is full, i.e. there is tubing and about 46 mL in the 50 mL tube, only about 11 mL will go to each tray. If using just 2 trays, only fill the 50 mL with about 22 mL of antibody. Actually, about 1 mL per tray will be lost. The clearing rinse step will replace the buffer in the tubing with the antibody, by pumping the antibody into the tubing. To make sure that there is practically no buffer left in the tubes, about 1 mL of antibody will be pumped out the other end of the tubing. When the Freedom Rocker tries to pump 30 mL, it will pump about 10 mL into each tray. After that, it is just pumping air since there is no more antibody to pump. By pumping air, most all of the antibody is forced into the trays.
One>Click™ Bottles, Caps, Tubes, and Fittings
One>Click™ Bottle Caps provide drip-free quick-release connections.
This is the easiest and safest way to connect and disconnect tubing from bottles. No pushing or pulling tube ends over cap ports and no more kinking or twisting of the tubing because the tubing can pivot freely.
- Reduce leaks and spills
- Reduce volatile fumes
- Reduce handling of hazardous reagents
FEATURES:
- Simple to connect and release. No forcing tubing over cap ports.
- Click sound confirms connections.
- Tubing pivots freely instead of kinking up.
- Sizes for 1/8, 3/16 and 1/4 tubing (inside diameter).
- O-ring seal at connection ensures no leakage.
- Valve in the cap prevents liquids from spilling out.
- Drip proof quick-release connections.
- Standard caps fit heavy duty Nalgene® bottles (shown on right) and Pyrex® Media bottles (shown on right). Caps are available for additional bottle types.
- Warranty: 2 years parts and labor.
- 30 day money back guarantee.
One>Click™ Operation:
To connect, gently push the fitting onto the cap. You will hear a click.
To disconnect, push in the tab. You will hear a click. Then slide the fitting off.
mouse over image above and click to watch video
Caps for many popular bottles:
We stock One>Click™ Caps for Nalgene® heavy duty bottles and carboys and Pyrex®media bottles. Caps for many other bottles are available.
Bottles & Caps
We carry a range of tubes and bottles with One>Click™ caps, from 50 mL centrifuge tubes and Pyrex® media bottles as small as 100 ml to large Nalgene carboys with 83B caps.
Fittings
These fittings mate with One>Click™ caps. You can attach them to standard soft tubing, such as silicone tubing. The inside diameter of the tubing that you are using determines which size fitting you need. They are available to fit 1/8, 3/16, and 1/4 inside diameter tubing.
BTLN52 Nalgene 2000ml Polyethylene Bottle with One Click Cap $85.00
BTLN54 Nalgene 4000ml Polyethylene Bottle with One Click Cap $70.00
BTLP61 250ml Pyrex Media Bottle with One Click Cap $47.00
BTLP62 500ml Pyrex Media Bottle with One Click Cap $50.00
TB50 50 mL centrifuge tube with One Click cap $23.00
CAPN53 size 53 cap for 1L and 2L Nalgene Heavy Duty Bottles $45.00
CAPN83 size 83B cap for 4L and Larger Nalgene Bottles and Carboys $60.00
CAPP45 for Pyrex Media Bottles $25.00
Trays $0.00
Tube Sets $0.00
Aspiration Heads $0.00
FTG tubing to bottle $11.00
Freedom Rocker BlotBot 120 $2,750.00
Freedom Rocker DX2 automated blot gel processor washer $3,400.00
Freedom Rocker DX4 automated blot gel processor washer $3,600.00
Freedom Rocker DX2 MD automated western blot gel processor washer $3,850.00
Freedom Rocker DX4 MD automated western blot gel processor washer $4,200.00
