Liver Homogenization in the Bullet Blender- Tissue Disruption, Cell Lysis, Protein, DNA, RNA Extraction, Whole Cell Isolation

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05.07.2012 The Bullet Blender can homogenize the toughest samples, even bone!

Researchers from Pennington Biomedical Research Center utilized the Bullet Blender to extract RNA from a mouse femur to examine gene expression in hig . . .
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1548 Burden Lake Road
Averill Park, NY 12018, USA

Phone: 1.518.674.3510
in USA 1.800.738.1681
Fax: 1.518.674.0189 info@nextadvance.com

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Ideal for Liver Samples
Save Time, Effort and Get Superior Results with


Consistent Results Samples Stay Cool No Cross Contamination Easy and Convenient Risk Free
Do you spend lots of time and effort homogenizing Liver samples? The Bullet Blender^® is a multi-sample homogenizer that delivers superior results. No other homogenizer comes close to delivering the Bullet Blender's winning combination of top-quality performance and budget-friendly affordability.
Consistent and High Yield Results Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g. No Cross Contamination No part of the Bullet Blender^® ever touches the Liver - the sample tubes are kept closed during homogenization. There are no probes to clean between samples. Samples Stay Cool Homogenizing causes only a few degrees of heating. Our "Blue" model comes with a fan to maintain ambient temperatures. Easy and Convenient to Use Just place beads and buffer along with your Mouse Femur sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start. Risk Free Purchase The Bullet Blender^® comes with a 30 day money back guarantee and a 2 year warranty, with a 3 year warranty on the motor. The simple, reliable design enables the Bullet Blenders to sell for a fraction of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

"It's a very useful device for making tissue lysates. We no longer use sonication for DNA, RNA and protein preparation from multiple tissue samples."

Prof. Michael Sherman, Boston University School of Medicine
"The Bullet Blender has made our clinical lab much more efficient; we get consistent results in less time. We cannot imagine going back to hand vortexing for our whole blood extractions."

Kimberly Dumont, supervisor, Dartmouth Reference Lab
"I can count on it for fast consistent results."

Dr. Denis Schewe, Mt. Sinai Medical Center, New York City
"What I've found is that the instrument works very well, is faster than the Bead Beater, accommodates more samples and is considerably quieter... [I] get consistently uniform cell breakage."

Dr. Joseph Geisberg, Harvard Medical School
"This past January [2008] we purchased a Bullet Blender from Next Advance. ... Let me begin by saying that we are extremely satisfied with the product! We have been able to implement it into a number of protocols that we use in our lab. Other labs in our department have also taken a liking to the unit and have been using it along side our lab."

Dr. Kyle Bender, Queen's University, Kingston, Ontario, CANADA
"The Bullet Blender lets us reduce variability and user error and increase productivity."

Dr. Scott Gridley, Director of Protein Sciences, Blue Sky Biotech, Inc., Worcester, MA
"Using the Dounce homogenizer, I can process eight tumors in an hour and a half. With the Bullet Blender, I can homogenize twenty in fifteen minutes."

Dr. Alejandro Adam, Albany Medical Center, New York
"Our extraction staff have loved the Bullet Blenders, they have really helped with the workflow!" ... "They do a great job homogenizing for us, and save our techs from repetititve motion injuries, and help with time management. We love them!"

Chantry Clark, CDAT Lead Technologist, ARUP Labs, Utah
"I love it!! ... I'm going to try and get another one."

Shari Kohut, MLT ASCP, Women and Children's Hospital of Buffalo
"We used to use a mortar and pestle grinder and it took hours and hours and hours to homogenize our samples. The Bullet Blender has increased our throughput tremendously."

Dr. Christopher Ward, University of Maryland
"The Bullet Blender homogenizes our samples so well, our protein yield is almost too good."

Dr. Donna Newman, North Carolina State University
"It does work great for westerns ... It's a lot faster."

Dr. Timothy Bartness, Georgia State University
"That machine is a lifesaver! ... It worked out so well, we're contemplating purchasing a second one."

Dr. Lou Casta, Fraunhofer USA
"...the results are great! ... We have run it on the Agilent chip and the RNA integrity numbers (RIN) are 10 and 8.6 for both of the samples."

Phuong Nguyen, Translational Genomics Group, Baylor Health
"We use the Bullet Blender to disrupt mycobacteria and different types of gram-negative bacteria for a variety of applications and are very happy with the results we get."

Martin Pavelka, Ph.D., University of Rochester, School of Medicine and Dentistry
"I love the Bullet Blender! We work on the mouse model for chlaymdia and often have to remove the uteri from the mice and homogenize by hand. I used it the other day and was amazed how well it homogenized the tissue. We can do 24 samples in just 5 minutes-amazing. What used to take us hours we can do in just 5 minutes."

Cheryl Champion, Ph.D., Pathology & Laboratory Medicine, UCLA

Bullet Blender settings for Liver

Sample size	Bullet Blender Model	Lysing Matrix	Homogenization Time
up to 100mg	BBX24 or BBX24B	Pink	Up to 6 minutes
100 - 300mg	BBX24 or BBX24B	Red	Up to 10 minutes
300mg - 1g	BBX5MB	See Protocol	Up to 10 minutes
1g - 3.5g	BB50-DX	See Protocol	Up to 15 minutes

liver organ tissue homogenizer homogenization homogenizing crush pulverize disrupt lyse extraction mill dounce bead beater

Before

After

Publications & References

Carlstrom M, Larsen FJ, Nystrom T, Hezel M, Borniquel S, Weitzberg E, Lundberg JO (2010) Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice. PNAS 10.1111/j.1530-0277.2010.01364
Choi HS, Nasr K, Alyabyev S, Feith D, Lee JH, Kim SH, Ashitate Y, Hyun H, Patonay G, Strekowski L, Henary M, Frangioni JV (2011) Synthesis and in vivo fate of zwitterionic near-infrared fluorophores. Angew Chem Int Engl 50:6258-63
Chuang HL, Huang YT, Chiu CC, Liao CD, Hsu FL, Huang CC, Hou CC (2011) Metabolomics characterization of energy metabolism reveals glycogen accumulation in gut-microbiota-lacking mice. J Nutr Biochem 10.1016/j.jnutbio.2011.03.019
Fox KA, Longo M, Tamayo E, Kechichian T, Bytautiene E, Hankins GDV, Saade GR, Costantine MM (2011) Effects Of Pravastatin On Mediators Of Vascular Function In A Mouse Model Of Soluble Fms-Like Tyrosine Kinase-1Induced Preeclampsia. Am J Obstet Gynecol doi:10.1016/j.ajog.2011.06.083/li>
Giera S, Bansal R, Ortiz-Toro TM, Taub DG, Zoeller RT (2011) Individual polychlorinated biphenyl (PCB) congeners produce tissue- and gene-specific effects on thyroid hormone signaling during development. Endocrinology 152(7):2909-19.
Gornicka A, Morris-Stiff G, Thapaliya S, Papouchado BG, Berk M, Feldstein AE (2011) Transcriptional profile of genes involved in oxidative stress and antioxidant defense in a dietary murine model of steatohepatitis. Antioxid Redox Signal 15: 437-45
Hwang JT, Kim SH, Hur HJ, Kim HJ, Park JH, Sung MJ, Yang HJ, Ryu SY, Kim YS, Cha MR, Kim MS, Kwon DY (2011) < a href="http://onlinelibrary.wiley.com/doi/10.1002/ptr.3612/full">Decursin, an Active Compound Isolated from Angelica gigas, Inhibits Fat Accumulation, Reduces Adipocytokine Secretion and Improves Glucose Tolerance in Mice Fed a High-Fat Diet. Phytother Res doi: 10.1002/ptr.3612
Lebold KM, Grant KA, Freeman WM, Wiren KM, Miller GW, Kiley C, Leonard SW, Traber MG (2010) Individual Differences in Hyperlipidemia and Vitamin E Status in Response to Chronic Alcohol Self-Administration in Cynomolgus Monkeys. Alcohol Clin Exp Res 10.1073/pnas.1008872107
Miller BF, Robinson MM, Bruss MD, Hellerstein M, Hamilton KL (2011) A comprehensive assessment of mitochondrial protein synthesis and cellular proliferation with age and caloric restriction. Aging Cell 10.1111/j.1474-9726.2011.00769
Rayner KJ, Esau CC, Hussain FN, McDaniel AL, Marshall SM, van Gils JM, Ray TD, Sheedy FJ, Goedeke L, Liu X, Khatsenko OG, Kaimal V, Lees CJ, Fernandez-Hernando C, Fisher EA, Temel RE, Moore KJ (2011) Inhibition of miR-33a/b in non-human primates raises plasma HDL and lowers VLDL triglycerides. Nature 478: 404-7
Rayner KJ, Suarez Y, Davalos A, Parathath S, Fitzgerald ML, Tamehiro N, Fisher EA, Moore KJ, Fernandez-Hernandomi C (2010) MiR-33 coordinates genes regulating cholesterol homeostasismiR-33 coordinates genes regulating cholesterol homeostasis.Science 328:1570-1573
Roberts SC, Noyes PD, Gallagher EP, Stapleton HM (2011) Species-Specific Differences and Structure-Activity Relationships in the Debromination of PBDE Congeners in Three Fish Species. Environ Sci TechnolFeb 3 [Epub ahead of print]
Smith JS, Xu Z, Tian J, Palmer D, Ng P, Byrnes AP (2011) The Role of Endosomal Escape and Mitogen-Activated Protein Kinases in Adenoviral Activation of the Innate Immune Response. PLoS one 6(10): e26755
Shai SY, Sukhanov S, Higashi Y, Vaughn C, Rosen C, Delafontaine, P (2010) Low circulating insulin-like growth factor I increases atherosclerosis in ApoE-deficient mice. Am J Physiol Heart Circ Physiol 300(5):H1898-906
Zahr NM, Luong R, Sullivan EV, Pfefferbaum A (2010) Measurement of Serum, Liver, and Brain Cytokine Induction, Thiamine Levels, and Hepatopathology in Rats Exposed to a 4-Day Alcohol Binge Protocol. Alcohol Clin Exp Res 34:1858-70

To learn more about the Bullet Blender click here

Next Advance - Innovative Lab Products for the Life Sciences

05.07.2012 The Bullet Blender can homogenize the toughest samples, even bone!

Ideal for Liver Samples

Save Time, Effort and Get Superior Results with

"It's a very useful device for making tissue lysates. We no longer use sonication for DNA, RNA and protein preparation from multiple tissue samples."

"The Bullet Blender has made our clinical lab much more efficient; we get consistent results in less time. We cannot imagine going back to hand vortexing for our whole blood extractions."

"I can count on it for fast consistent results."

"What I've found is that the instrument works very well, is faster than the Bead Beater, accommodates more samples and is considerably quieter... [I] get consistently uniform cell breakage."

"The Bullet Blender lets us reduce variability and user error and increase productivity."

"Using the Dounce homogenizer, I can process eight tumors in an hour and a half. With the Bullet Blender, I can homogenize twenty in fifteen minutes."

"Our extraction staff have loved the Bullet Blenders, they have really helped with the workflow!" ... "They do a great job homogenizing for us, and save our techs from repetititve motion injuries, and help with time management. We love them!"

"I love it!! ... I'm going to try and get another one."

"We used to use a mortar and pestle grinder and it took hours and hours and hours to homogenize our samples. The Bullet Blender has increased our throughput tremendously."

"The Bullet Blender homogenizes our samples so well, our protein yield is almost too good."

"It does work great for westerns ... It's a lot faster."

"That machine is a lifesaver! ... It worked out so well, we're contemplating purchasing a second one."

"...the results are great! ... We have run it on the Agilent chip and the RNA integrity numbers (RIN) are 10 and 8.6 for both of the samples."

"We use the Bullet Blender to disrupt mycobacteria and different types of gram-negative bacteria for a variety of applications and are very happy with the results we get."

Bullet Blender settings for Liver

Sample size

Bullet Blender Model

Lysing Matrix

Homogenization Time

Before

After

Publications & References