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>
No Cross Contamination
The samples are homogenized (or mixed)
non-invasively. The sample tubes are kept closed during
agitation, as the samples are processed. There are no probes to
clean between samples.
> Samples Stay Cool
The instrument uses very little power due to the
unique, patented design. It uses a small DC motor to agitate the
individual tubes, not ultrasonics. Also, because the motor
does not need to agitate any heavy platforms or plates, it is small and
will last for years.
>
Convenient
to Use
Simply place your adipose and other tissue samples or
cell cultures and some beads in standard high quality polypropylene
tubes, and load the tubes into the Bullet Blender™.
Set the duration (typically a few minutes) and speed
(vigorousness). There are no probes to clean. And it is
quieter than a sonicator and does not heat up your samples more than a
few degrees!
>
Risk Free
Purchase
The Bullet
Blender™ comes with a 30-day
money back guarantee and a two year warranty, with a three year
warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction
of the price of ultrasonic or other agitation based instruments, yet
provides an easier, quicker technique.
> Established Protocols
Protocol for Adipose Tissue Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the
homogenization of fat / adipose tissue (from a variety of animals). Note that the time and
speed settings may differ due to the variation in consistency/texture of fatty tissue from
species to species. This protocol does not specify a particular buffer - you may choose
which is most appropriate for your downstream application (nucleic acid isolation, protein
extraction, etc.).
Materials Required:
adipose tissue, saline, aspirator, Bullet Blender™,
zirconium oxide beads (0.5mm OR 1.0mm) ,
microcentrifuge tubes, homogenization buffer, and pipetor.
Instructions:
- Cut adipose tissue into appropriately sized pieces for analysis (30mg-300mg) and
place into a microcentrifuge tube.
- OPTIONAL: Wash tissue with ~1mL PBS. Aspirate. NOTE: This step removes
external contaminants (blood, etc.).
- Add a mass of 0.5mm zirconium oxide beads equal to the mass of sample in each tube (so for 100mg sample, add 100mg beads). One scoop ≈ 180mg.
- Add 2 - 4 volumes of buffer for every volume of tissue.
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 7 and TIME 2 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Inspect samples. Fatty tissue homogenate will be difficult to see through due to the
light scattering of lipid micelles formed, so it may be necessary to employ a pipette
tip to check inside the tube for remaining pieces of intact tissue. If homogenization
is unsatisfactory, run for another minute at SPEED 9.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
Special thanks to Dr. Timothy Bartness and Yang Liu at Georgia State University for their feedback on this protocol.
Protocol for Adipose in Bullet Blender™ 50 Homogenization
The protocol described in this document is for the use of the Bullet Blender™ 50 for the
homogenization of Fat / Adipose Tissue. This protocol does not specify a particular buffer
- you may choose which is most appropriate for your downstream application (nucleic acid
isolation, protein extraction, etc.).
Materials Required:
lung , Bullet Blender™ 50, homogenization buffer, pipettor,
50mL centrifuge tubes, 3.2mm stainless steel beads (part
number SSB32).
Instructions:
- Cut adipose into appropriately sized pieces for analysis (0.1g – 3g) and place into a
50mL centrifuge tube.
- OPTIONAL: If desired, wash the tissue 3x with 5mL PBS to remove blood and
other contaminants from the tissue.
- Add two times as much 3.2mm stainless steel beads (by mass) as you have sample
(so for 1g sample, add 2g beads). One scoop ≈ 160mg.
- Add three volumes of buffer per mass of tissue (3mL of buffer per gram of tissue)
- Tightly screw the centrifuge tubes closed.
- Place tubes into the Bullet Blender™ 50.
- Set controls for SPEED 10 and TIME 9 minutes. Press start.
- After the run, remove the tubes from the instrument.
- Inspect samples. Fatty tissue homogenate will be difficult to see through due to the
light scattering of lipid micelles formed, so it may be necessary to employ a pipette
tip to check inside the tube for remaining pieces of intact tissue. If homogenization is unsatisfactory, run for another six
minutes at SPEED 10.
- Proceed with your downstream application
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
Typical Results
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