The Bullet Blender™ and Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 hair and other tissue/cell samples at a time.  Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender ™.  Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the tissue samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively.  The sample tubes are kept closed during agitation, as the samples are processed.  There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your hair and other tissue samples or cell cultures and some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™.   Set the duration (typically a few minutes) and speed (vigorousness).  There are no probes to clean.  And it is quieter than a sonicator and does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30-day money back guarantee and a two year warranty, with a three year warranty on the motor.  The simple, reliable design enables the Bullet Blenders™ to sell for a fraction  of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

> Established Protocols

Protocol for Hair Homogenization in the Bullet Blender™

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of hair (from a variety of animals). Note that the time and speed settings may differ due to the variation in consistency/texture of hair from species to species. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

hair sample, saline, Bullet Blender™, heating block, homogenization buffer (8M urea, 50mM DTT, 50mM Tris Hcl), pipetor, microcentrifuge tubes, and 0.5mm glass beads (product number GB05)

Instructions:

1. Load 25mg hair into a microcentrifuge tube.
2. Add 0.1mL glass beads (0.5mm) to the tube. One scoop of beads ≈ 50μL.
3. Add 1.0mL buffer.
4. Close the centrifuge tubes.
5. Place tubes into a 95°C heating block for ten minutes.
6. Place tubes into the Bullet Blender™.
7. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
8. After the run, remove tubes from the instrument.
9. Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at the SPEED 8.
10. Proceed with your downstream application.

SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

Typical Results