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>
No Cross Contamination
The samples are homogenized (or mixed)
non-invasively. The sample tubes are kept closed during
agitation, as the samples are processed. There are no probes to
clean between samples.
> Samples Stay Cool
The instrument uses very little power due to the
unique, patented design. It uses a small DC motor to agitate the
individual tubes, not ultrasonics. Also, because the motor
does not need to agitate any heavy platforms or plates, it is small and
will last for years.
>
Convenient
to Use
Simply place your gastric tissue and other tissue samples or
cell cultures and some beads in standard high quality polypropylene
tubes, and load the tubes into the Bullet Blender™.
Set the duration (typically a few minutes) and speed
(vigorousness). There are no probes to clean. And it is
quieter than a sonicator and does not heat up your samples more than a
few degrees!
>
Risk Free
Purchase
The Bullet
Blender™ comes with a 30-day
money back guarantee and a two year warranty, with a three year
warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction
of the price of ultrasonic or other agitation based instruments, yet
provides an easier, quicker technique.
> Established Protocols
Protocol for Jejunum or Stomach Tissue Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the
homogenization of jejunum or stomach / gastric tissue. Note that the time and speed
settings, and digestion parameters may differ due to the variation in consistency/texture
of tissue from species to species. This protocol does not specify a particular buffer - you
may choose which is most appropriate for your downstream application (nucleic acid
isolation, protein extraction, etc.).
Materials Required:
tissue, cell culture hood, Bullet Blender™,
homogenization buffer, microcentrifuge tubes, pipettor,
and 0.5mm glass beads (part number GB05).
Instructions:
- Cut tissue into appropriately sized pieces for analysis (100mg) and place into a
microcentrifuge tube. NOTE: Try to remove pieces of connective tissue as they do
not homogenize well.
- OPTIONAL: Wash tissue 3x with ~1mL PBS. Aspirate. NOTE: This step removes
any external contaminants (blood, undigested food, etc.).
- Flash freeze freeze tissue in liquid nitrogen or dry ice/alcohol bath.
- When ready to homogenize, place tissue in ice bucket to proceed.
- Add glass beads (0.5mm) to the tube. Use a mass of beads equal to your mass of
tissue. One scoop of beads ≈ 77mg.
- Add about 0.3mL buffer (2 volumes of buffer for every volume of cells).
- Close the centrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME 2 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Visually inspect samples. If homogenization is unsatisfactory, run for another two
minutes at the SPEED 10.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
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