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>
No Cross Contamination
The samples are homogenized (or mixed)
non-invasively. The sample tubes are kept closed during
agitation, as the samples are processed. There are no probes to
clean between samples.
> Samples Stay Cool
The instrument uses very little power due to the
unique, patented design. It uses a small DC motor to agitate the
individual tubes, not ultrasonics. Also, because the motor
does not need to agitate any heavy platforms or plates, it is small and
will last for years.
>
Convenient
to Use
Simply place your pine nuts and other tissue samples or
cell cultures and some beads in standard high quality polypropylene
tubes, and load the tubes into the Bullet Blender™.
Set the duration (typically a few minutes) and speed
(vigorousness). There are no probes to clean. And it is
quieter than a sonicator and does not heat up your samples more than a
few degrees!
>
Risk Free
Purchase
The Bullet
Blender™ comes with a 30-day
money back guarantee and a two year warranty, with a three year
warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction
of the price of ultrasonic or other agitation based instruments, yet
provides an easier, quicker technique.
> Established Protocols
Protocol for Pine Nut Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the
homogenization of pine nut (seeds from the genuus Pinus). Note that the time and speed
settings may differ due to the variation in consistency/texture of different species. This
protocol does not specify a particular buffer - you may choose which is most appropriate
for your downstream application (nucleic acid isolation, protein extraction, etc.).
Materials Required:
pine nuts, saline, aspirator, Bullet Blender™, homogenization
buffer, pipettor, microcentrifuge tubes, 0.5mm zirconium oxide
beads (part number ZrOB05)
Instructions:
- Place one nut (100-200mg) into a microcentrifuge tube.
- OPTIONAL: Wash nut 3x with ~1mL PBS. Aspirate. NOTE: This step removes
some external contaminants and debris.
- Add 0.6g of zirconium oxide beads (0.5mm) to the tube. One scoop of beads ≈ 190mg.
- Add 0.4mL buffer to the tube.
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 10 and TIME 4 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Visual inspection of the homogenate is difficult due to the formation of emulsions
with the oils from the plant tissue in aqueous buffers. To verify sufficient
homogenization, you may place forceps or a spatula inside the tube to search for
remaining large fragments. If homogenization is unsatisfactory, run for another
two minutes at the SPEED 10.
- Remove sample tubes from the Bullet Blender™ and proceed with your downstream
application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
Typical Results
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