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Innovative Lab Products for the Life Sciences

The Bullet Blender™ and Bullet Blender™ Blue
          Spare your own muscles and homogenize
                     Muscle Tissue quickly and easily
in The Bullet Blender™!
Your Muscle Tissue samples stay cool
during homogenization!



The Bullet Blender™Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.

> No Cross Contamination
> Samples stay cool
> Convenient
> Risk  Free
> Established protocols

Homogenize up to 24 Muscle Tissue samples simultaneously-- in less than 3 minutes!

 

The Bullet Blender™ enables you to homogenize up to 24 samples (muscle or other tissues) in separate microcentrifuge tubes at a time.  Load up to 300mg Muscle Tissue with your application buffer and grinding beads in standard polypropylene microcentrifuge tubes.  Place the tubes into the Bullet Blender™ (pictured above) or Bullet Blender™ Blue.  The "bullets" in the "blender" vigorously strike all the sample tubes as the instrument runs for a few minutes.  The agitation of the grinding agents with the tissues homogenizes the samples.


The Bullet Blender™ and Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 muscle and other tissue/cell samples at a time, and the Bullet Blender™ Blue 50 allows you to homogenize 9 larger samples of up to about 5 grams of tissue. Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender ™.  Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the tissue samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively.  The sample tubes are kept closed during agitation, as the samples are processed.  There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your muscle tissue and other tissue samples or cell cultures and some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™.   Set the duration (typically a few minutes) and speed (vigorousness).  There are no probes to clean.  And it is quieter than a sonicator and does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30-day money back guarantee and a two year warranty, with a three year warranty on the motor.  The simple, reliable design enables the Bullet Blenders™ to sell for a fraction  of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

> Established Protocols

Protocol for Striated Muscle Tissue Homogenization in the Bullet Blender™

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of striated / skeletal muscle tissue (from a variety of animals). Note that the time and speed settings may differ due to the variation in consistency/texture of tissue from species to species. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

muscle tissue, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 0.5mm zirconium oxide beads (part number ZrOB05).

Instructions:
  1. Cut muscle tissue into appropriately sized pieces for analysis (50mg-300mg) and place into a microcentrifuge tube. Typical sample size: 200mg.
  2. OPTIONAL: Wash tissue 3x with ~1mL PBS. Aspirate. NOTE: This step removes external contaminants (blood, etc.).
  3. Add zirconium oxide beads (0.5mm) to the tube. Use a mass of beads equal to 1.5x to 2x your mass of tissue. One scoop of zirconium oxide beads ≈ 190mg.
  4. Add 0.1mL to 0.6mL buffer (2 volumes of buffer for every mass of tissue).
  5. Close the microcentrifuge tubes.
  6. Place tubes into the Bullet Blender™.
  7. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
  8. After the run, remove tubes from the instrument.
  9. Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at the SPEED 10.
  10. Proceed with your downstream application.
SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

 

Want to see a published protocol for muscle homogenization in the Bullet Blender?


Chiang J, Shen YC, Wang YH, Hou YC, Chen CC, Liao JF, Yu MC, Juan CW, Liou KT. Honokiol protects rats against eccentric exercise-induced skeletal muscle damage by inhibiting NF-?B induced oxidative stress and inflammation. Eur. J. Pharmacol. 2009 May 21;610(1-3):119-27



Bullet Blender™ 50 Homogenization Protocol for Striated Muscle

The protocol described in this document is for the use of the Bullet Blender™ 50 for the homogenization of Striated / Skeletal Muscle Tissue. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

Muscle , Bullet Blender™ 50, homogenization buffer, pipettor, 50mL centrifuge tubes, 4.8mm or 3.2mm stainless steel beads (part number SSB48 or SSB32).

Instructions:
  1. Cut adipose into appropriately sized pieces for analysis (0.1g – 3g) and place into a 50mL centrifuge tube.
  2. OPTIONAL: If desired, wash the tissue 3x with 5mL PBS to remove blood and other contaminants from the tissue.
  3. Add two times as much 4.8mm or 3.2mm stainless steel beads (by mass) as you have sample (so for 1g sample, add 2g beads). A 3.2mm bead weighs 136mg. A 4.8mm bead weighs 457g.

  4. Add two volumes of buffer per mass of tissue (2mL of buffer per gram of tissue)
  5. Tightly screw the centrifuge tubes closed.
  6. Place tubes into the Bullet Blender™ 50.
  7. Set controls for SPEED 10 and TIME 15 minutes. Press start.
  8. After the run, remove the tubes from the instrument.
  9. Visually inspect samples, if homogenization is unsatisfactory, run for another nine minutes at SPEED 10.
  10. Proceed with your downstream application.
SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

Typical Results
Typical Results

 


The Bullet Blender™ home page shows the entire family of products and links to other applications.