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No Cross Contamination
The samples are homogenized (or mixed)
non-invasively. The sample tubes are kept closed during
agitation, as the samples are processed. There are no probes to
clean between samples.
> Samples Stay Cool
The instrument uses very little power due to the
unique, patented design. It uses a small DC motor to agitate the
individual tubes, not ultrasonics. Also, because the motor
does not need to agitate any heavy platforms or plates, it is small and
will last for years.
> Convenient
to Use
Simply place your zebrafish and other small organisms, tissue samples, or
cell cultures along with some beads into standard high quality polypropylene
tubes and load the tubes into the Bullet Blender™.
Set the duration (typically a few minutes) and speed
(vigorousness). There are no probes to clean. And it is
quieter than a sonicator and does not heat up your samples more than a
few degrees!
> Risk Free
Purchase
The Bullet
Blender™ comes with a 30-day
money back guarantee and a two year warranty, with a three year
warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction
of the price of ultrasonic or other agitation based instruments, yet
provides an easier, quicker technique.
> Protocol development assistance
We can help you use the Bullet
Blender™,
if protocols are lacking for your application. Our Technical
Support staff will advise you on setting up your experiment as well as
troubleshooting the experiments which didn't turn out like you planned.
Homogenization Applications
- Downstream use (after homogenization)
- DNA extraction
- RNA extraction
- protein analysis
- small molecule isolation
The Bullet
Blender™ home page shows the entire family of products and links to
other applications.
Protocol for Zebrafish (D. Rerio) Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the
homogenization of embryonic or larval zebrafish (Danio rerio) This protocol has been
tested with fish younger than one week post-fertilization, but should be appropriate for
fish up to about one month post-fertilization. This protocol does not specify a
particular buffer - you may choose which is most appropriate for your downstream
application (nucleic acid isolation, protein extraction, etc.).
Materials Required:
zebrafish, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and Zirconium Oxide beads (0.5mm or 1.0mm).
Instructions:
- Place 10-300mg of zebrafish into microcentrifuge tubes. NOTE: Unhatched fish do not require prior dechorionation.
- Add a mass of zirconium oxide beads (0.5mm or 1.0mm) to the tube equal to 2x
your mass of sample (so for 50mg of zebrafish, add 100 mg of beads).
One scoop of beads ≈ 180mg.
- Add 2 volumes of buffer for every mass of fish (for example, with 100mg of fish use 200ml of buffer).
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME 3 minutes.
- Remove tubes from the instrument.
- Visually inspect samples, if homogenization is unsatisfactory, run for another two minutes at the SPEED 8.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
Typical Results
 
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