|
>
No Cross Contamination
The samples are homogenized (or mixed) non-invasively. The
sample tubes are kept closed during agitation, as the
samples are processed. There are no probes to
clean between samples.
>
Samples Stay
Cool
The instrument uses very
little power due to the unique, patented design.
It uses a small DC motor to agitate the individual
tubes, not ultrasonics. Also, because
the motor does not need to agitate any heavy
platforms or plates, it is small and will last for
years.
>
Convenient to Use
Simply place your tumors and other tissue samples or cell cultures and some beads in standard
high quality polypropylene tubes, and load the tubes into the
Bullet Blender™. Set the duration (typically a few
minutes) and speed (vigorousness). There are no probes to clean.
And it is quieter than a sonicator and does not heat up
your samples more than a few degrees!
>
Risk Free Purchase
The Bullet Blender™
comes with a 30-day money back guarantee and a two year
warranty, with a three year warranty on the motor. The simple, reliable design enables the
Bullet Blenders™ to
sell for a fraction of the price of ultrasonic or
other agitation based instruments, yet provides an easier,
quicker technique.
> Established Protocols
Rat
aorta: Load 3 stainless steel beads (1.6 mm dia) in each
microcentrifuge tube. Add 25 - 50μL buffer (your choice,
depending on your downstream application). Place dissected tissue
into the buffer, then place the tube into the Bullet
Blender™. Set the time to 4 minutes and the speed to 8.
Mouse
Heart: Place the entire heart (~200mg) into a microcentrifuge tube.
Add stainless steel grinding beads (either 1.6mm or 0.5-2.0mm
blend, 200mg), then the buffer of your choice (twice the mass of
tissue, i.e. ~400μL). Place the tube into Bullet Blender™. Set the time to 4 minutes and the speed to 8.
 |
 |
Heart tissue (0.3g) before homogenization
with stainless beads (1.6mm, 0.3g)
with 0.6mL buffer (0.5% NP-40)
|
Protein
concentration
(mg/mL) |
28.1
|
22.5
|
28.6
|
28.4
|
|
|
After heart homogenization
with stainless beads, 4 minutes speed 8,
centrifuged 15 minutes @ 14K rpm
|
The
Bullet Blender™ home page shows the entire family of products and links to other applications. |
