Bullet Blender. Homogenize tumors, carcinomas, lyse or disrupt cells.

Automated
Blot Processor

Capillary
Loader

Syringe Pumps

Homogenizer
Cell Disrupter

Design by DanubeNet

The easiest and coolest way to harvest DNA, RNA and proteins from tumors

Your tumor samples stay cool!

The Bullet Blender™ Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.

> No Cross Contamination

> Samples stay cool

> Convenient

> Risk Free

Homogenize up to 24 tumor samples simultaneously, in just minutes!

The Bullet Blenders™ enable you to homogenize up to 24 tumor or other samples in microcentrifuge tubes at a time. Load up to .25g of tissue, along with some beads and buffer, in standard polypropylene tubes into the Bullet Blender™ (above) or the Bullet Blender™ Blue. The "bullets" in the "blender" vigorously strike all the sample tubes simultaneously for a few minutes. The agitation homogenizes the samples.

RT-PCR results of RNA from an epithelial carcinoma
homogenized with the Bullet Blender™.

Homogenize tumors and other tissue and cell culture samples with the Bullet Blender.

Control transcript GAPDH (glyceraldehyde 3-phosphate dehydrogenase) does not fluctuate while the transcript of interest varies between samples. High quality RNA is isolated, shown by A_260/280 and A_260/230 near 2.0.

Data provided by Dr. Denis Schewe

“I can count on it for fast consistent results.”

Dr. Denis Schewe, Mt. Sinai Medical Center, New York City

The Bullet Blender™ and the Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 tumor and other tissue/cell samples at a time. Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender™. Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the plant samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively. The sample tubes are kept closed during agitation, as the samples are processed. There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design. It uses a small DC motor to agitate the individual tubes, not ultrasonics. Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your tumors and other tissue samples or cell cultures and some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™. Set the duration (typically a few minutes) and speed (vigorousness). There are no probes to clean. And it is quieter than a sonicator and does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30 day money back guarantee and a 2 year warranty, with a 3 year warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

Click here to go to the main Bullet Blender™ web page.

Protocol for tumor tissue RNA extraction

Prepare one microcentrifuge tube with 5 stainless steel beads (about 1 scoop, 1.6mm diameter beads) for each sample.

Add 1mL Trizol® into each tube, followed by the tumor sample (approximately 4mm x 4mm x 4mm).

Process immediately in the Bullet Blender, speed 10 for 5 minutes. If appearance of the sample homogenate is not satisfactory, run for another 5 minutes.

Proceed with manufaturer's protocol for Trizol® RNA extraction.