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The easiest and
coolest
way to
homogenize E. coli
No chemical lysis or harsh detergents required!
Your E. coli samples stay cool! |
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The Bullet Blender™
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No Chemical Lysis or
Harsh Detergents
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No Cross
Contamination
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Samples stay
cool
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Convenient
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Risk Free
Lyse many E. coli samples at the same time, in just minutes!
The
Bullet Blender™ enables
you to lyse up to 24 E. coli cultures in microcentrifuge
tubes or up to 9
E. coli
cultures in 50 mL tubes at a time.
Load the cultures along with beads in standard polypropylene tubes into the
Bullet Blender™
(shown above on the left) or
Bullet Blender™ Blue 50
(shown above on the right). The "bullets" in the
"blender" vigorously strike all the sample
tubes simultaneously for a few minutes.
The agitation homogenizes the samples. The
easiest way to harvest native proteins or nucleic
acids. |
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“The Bullet Blender™ lets us reduce
variability and user error and
increase productivity.”
Dr. Scott Gridley, Director of
Protein Sciences, Blue Sky Biotech,
Inc., Worcester, MA
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Data provided by Blue
Sky Biotech, Inc., Worcester, MA
www.blueskybiotech.com |
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The Bullet Blender™ microtube models
holds up to 24 E. coli cultures in microcentrifuge
tubes at a time.
Process times of 3 to 5 minutes for
disrupting E. coli. See our
suggested protocols
for lysing E. coli and disrupting other cells and tissue.
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The Bullet Blender™ 50
mL models
holds up to 9
E. coli cultures in 50 ml tubes at a time.
The sample, lysis buffer, and beads should occupy no
more than 15 ml of any tube. Process times
of 6 to 10 minutes are typical for disrupting E.
coli cultures.
suggested protocol.
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The
Bullet Blender™ and
the Bullet Blender™ Blue
enable
you to disrupt or homogenize up to 24 E. coli
or other tissue/cell
samples at a time. Load the E. coli
cultures or other samples in
standard polypropylene microcentrifuge tubes into the Bullet
Blender™.
Likewise, the
Bullet Blender™Blue 50
can disrupt or homogenize up to 9 E. coli or other samples at a time loaded in 50 ml polypropylene
tubes. Balls (the "bullets") repeatedly strike the sample
tubes in a controlled manner, thousands of times per
minute, inducing vigorous motion of the E. coli
cultures or other substances inside the tubes. This provides efficient mixing. With beads
in the sample tubes, the cell cultures, or tissues, are thoroughly homogenized. Tune the
extent of disruption by adjusting the speed.
Click here to see sample protocols. |
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No Chemical
Lysis or Harsh Detergents Required
The Bullet Blender™
lyses E. coli and other cells using mechanical action so no alkaline
buffers are necessary. See the
protocols for preparation times and speeds.
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No Cross Contamination
The E. coli or other samples are disrupted (or mixed) non-invasively. The
sample tubes are kept closed during agitation, as the
cell cultures are processed. There are no probes to
clean between samples.
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Samples Stay
Cool
The instrument uses very
little power due to the unique, patented design.
It uses a small DC motor to agitate the individual
tubes, not ultrasonics. Also, because
the motor does not need to agitate any heavy
platforms or plates, it is small and will last for
years.
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Convenient to Use
Simply place your E. coli
or other cell cultures and some beads in standard
high quality polypropylene tubes, and load the tubes into the
Bullet Blender™.
Set the duration (typically
a few
minutes) and speed (vigorousness). There are no probes to clean.
And it is quieter than a sonicator and does not heat up
your samples more than a few degrees!
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Risk Free Purchase
The Bullet Blender™
comes with a 30 day money back guarantee and a 2 year
warranty, with a 3 year warranty on the motor. The simple, reliable design enables the
Bullet
Blenders™ to
sell for a fraction of the price of ultrasonic or
other agitation based instruments, yet provides an easier,
quicker technique.
Click here to go to the main
Bullet Blender™ web page. |
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Protocol for E. coli Cultures Homogenization in the Bullet Blender™ The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of Escherichia coli (or other bacterial) cultures. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.). Materials Required: E. coli, aspirator, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 0.15mm zirconium oxide beads (part number ZrOB015) or 0.1mm glass beads (part number GB01) Instructions: - Pour overnight bacterial culture into a microcentrifuge tube.
- Centrifuge culture to yield a cell pellet (2000g for one minute).
- Completely aspirate the supernatant liquid. Place tube on ice.
- Inspect the volume of the pellet. It should be 300µL or less in order to get efficient homogenization.
- Add an equal volume of zirconium oxide beads (0.15mm) OR glass beads (0.1mm) to the tube. One scoop of beads ≈ 50μL. See NOTES below.
- Add 0.1mL to 0.6mL buffer (2 volumes of buffer for every volume of cells).
- Snap the caps centrifuge tubes closed.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME to 3 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at the SPEED 8.
- Proceed with your downstream application.
SAFETY NOTE!!! When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced. Notes Different species of bacteria and different applications will be amenable to different bead types. Cell density, cell size, and buffer composition will affect homogenization and variation of the bead selection is an easy way to empirically determine what works best. For tougher cells, we recommend using zirconium oxide beads. Increasing the speed to 10 will also help homogenize tougher cell cultures.
Protocol for E. coli Cultures Homogenization in the Bullet Blender™ 50 The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of Escherichia coli (or other bacterial) cultures. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.). Materials Required: E. coli, aspirator, Bullet Blender™ 50, beads (zirconium oxide 0.15mm diameter – part number ZrOB015 OR glass 0.1mm diameter – part number GB01), homogenization buffer, 50mL centrifuge tubes, and pipettor. Instructions: - Pour overnight bacterial culture into a 50mL centrifuge tube.
- Centrifuge culture to yield a cell pellet (2000g for one minute).
- Completely aspirate the supernatant liquid. Place tube on ice.
- You may use a larger cell pellet than yielded from 50mL culture by loading more culture, and then repeating steps 1 through 3. NOTE: Increasing pellet may require a longer homogenization time to yield the same homogenization efficiency.
- Inspect the volume of the pellet. It should be 5mL or less in order to get efficient homogenization.
- Add an equal volume of zirconium oxide beads (0.15mm) OR glass beads (0.1mm) to the tube. See NOTES below.
- Add buffer (2 volumes of buffer for every volume of cells).
- Close centrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME to 3 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at the SPEED 8.
- Proceed with your downstream application.
SAFETY NOTE!!! When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced. Notes Different species of bacteria and different applications will be amenable to different bead types. Cell density, cell size, and buffer composition will affect homogenization and variation of the bead selection is an easy way to empirically determine what works best.
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