Bullet Blender. Homogenize plant leaves, stems, roots, seeds, tissue, tissue homogenization, high throughput, homogenization

Automated
Blot Processor

Capillary
Loader

Syringe Pumps

Homogenizer
Cell Disrupter

Design by DanubeNet

The easiest and coolest way to harvest DNA, RNA and proteins from plants

No chemical lysis or harsh detergents required!
Your plant samples stay cool!

The Bullet Blender™ Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.

> No Chemical Lysis or Harsh Detergents

> No Cross Contamination

> Samples stay cool

> Convenient

> Risk Free

Homogenize up to 24 samples of plant matter simultaneously, in just minutes!

The Bullet Blenders™ enable you to homogenize up to 24 plant samples in microcentrifuge tubes at a time. Load the leaf clippings, stem pieces, etc., along with beads and buffer, in standard polypropylene tubes into the Bullet Blender™ (shown above) or the Bullet Blender™ Blue. The "bullets" in the "blender" vigorously strike all the sample tubes simultaneously for a few minutes. The agitation homogenizes the samples.

DNA from Tobacco and Rice leaves homogenized in the Bullet Blender™.

Homogenize plant specimens such as leaves, with the Bullet Blender

Genomic DNA isolated from Arabidopsis thaliana and Orzya sativa leaves. Note the large DNA fragments.

Data provided by Integrated Plant Genetics, Alachua, FL www.ipgenetics.com

“This past January we purchased a Bullet Blender from Next Advance. ... Let me begin by saying that we are extremely satisfied with the product! We have been able to implement it into a number of protocols that we use in our lab. Other labs in our department have also taken a liking to the unit and have been using it along side our lab.”

“We have been homogenizing mostly Arabidopsis thaliana leaf tissues and A. thaliana seedlings.”

Dr. Kyle Bender, Queen's University, Kingston, Ontario, CANADA

The Bullet Blender™

holds up to 24 plant samples in microcentrifuge tubes at a time. Process times of 3 to 5 minutes for homogenizing leaf clippings, stems, roots, seeds, etc. See our suggested protocols for plant tissue and disrupting other cells and tissue.

The Bullet Blender™ and the Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 plant or other tissue/cell samples at a time. Load the plant matter or other samples in standard polypropylene microcentrifuge tubes into the Bullet Blender™. Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the plant samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Chemical Lysis or Harsh Detergents Required

The Bullet Blender™ homogenizes plant tissue and other cells using mechanical action so no alkaline buffers are necessary. See the protocols for preparation times and speeds.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively. The sample tubes are kept closed during agitation, as the samples are processed. There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design. It uses a small DC motor to agitate the individual tubes, not ultrasonics. Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your plant clippings or other tissue samples or cell cultures and some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™. Set the duration (typically a few minutes) and speed (vigorousness). There are no probes to clean. And it is quieter than a sonicator and does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30 day money back guarantee and a 2 year warranty, with a 3 year warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

Click here to go to the main Bullet Blender™ web page.

Protocols from customers:

Place 4 ZrO beads, 2mm diameter, in a 2.0 mL microtube, along with 250 uL of buffer. Snap 1/2 cap of a fresh green leaf. Then add another 250 uL of buffer. (If you add all the buffer before the leaf sample, the leaf will float). Run at speed 8 for 3 minutes.

Dr. Joseph Reddy, Integrated Plant Genetics, Alachua, FL

We have been homogenizing mostly Arabidopsis thaliana leaf tissues and A. thaliana seedlings. We are using the Bullet Blender mainly for tissue homogenization prior to protein and genomic DNA extractions. We have been using the 1.6mm stainless steel beads for this purpose. Prior to tissue collection we place 3 or 4 beads into the microcentrifuge tube. We then collect the tissue and snap freeze the tube in liquid nitrogen. Once frozen we place tubes into the bullet blender and pulse the samples 3 or 4 times to disrupt the tissue. After adding extraction buffer (whatever amount the protocol calls for, usually around 450-500uL) we homogenize in the blender at speed 8 for 2 or 3 minutes. This extraction step has yielded high quality results in beta-GUS fluorimetric assays and has made PCR-screening transgenic lines a much smoother process.

Dr. Kyle Bender, Queen's University, Kingston, Ontario