Get Better LC/MS Results and Save Money Too!
Pack your own LC/MS capillary columns.
See our capillary column packing kits. Load your samples into mass spectrometers without transfer loss or contact with metallic surfaces. The Pressure Injection Cell, sometimes called a “bomb loader” enables controlled dispensing of small-volume liquid samples. Using high pressure, the pressure injection cell has proved invaluable for two applications: densely packing nanobore capillary columns with solid-phase particles; and precisely infusing microliter samples directly from microcentrifuge tubes into mass spectrometers without additional transfers, wasted sample, or contact with metallic surfaces which adsorb some negatively charged molecules such as peptides with phospho or carboxy groups. The Pressure Injection Cell holds 1 mL and 2 mL micro-centrifuge tubes as well as 12 x 32 mm glass vials in its central chamber (as shown at right). The assembly can be used on a typical magnetic stirrer, e.g. to keep particles in suspension. The Pressure Injection Cells accept various sample tubes, including 0.5 mL, 1.5 mL, and 2.0 mL microcentrifuge tubes and 12 mm diameter glass vials. The cell needs to be connected to a bottle/tank of compressed gas, such as Helium or Nitrogen. Packing capillary columns requires several hundred to about 1000 psi or sometimes more, and mass spectrometer injections typically require a few hundred psi. This unit is rated for a 2500 psi. 8500 psi units are available.
Why pay hundreds of dollars per packed capillary column for LC/MS? You can pack the columns, of various internal diameters, yourself. The Pressure Injection Cell comes with instructions on how to pack columns and is available as a stand alone unit (with 9 extra ferrules), or as a complete kit with ferrules, 1/8″ stainless steel tubing, a spool of capillary, a cleaving tool, and a frit assembly, or as a partial kit.
Download the Pressure Injection Cell brochure.
“We are very happy with the performance of our Next Advance pressure cell. Here are two representative total ion chromatograms generated from a capillary column using sub 2 micron, C18 particles and analyzed on a UPLC. As you can see the peak width is around 15 sec or less. We are getting this kind of resolution routinely from the columns packed by your pressure cell”
–Dr. Austin Yang, University of Maryland
“The pressure injection cell is really handy. I have two in my lab, one for packing capillaries and one for loading samples in my mass spec. Packing my own capillaries saves me hundreds of dollars on each one.”
–Dr. Qishan Lin, Director of the Proteomics Core Facility The Center for Functional Genomics,
University at Albany, New York
“Hey, it works great. I am getting much better resolution because of packing my own columns and using the cell to directly inject my samples. The first purchase in a long time that was well worth the money. It is well made and so easy to use. After I purchased the fittings to tie it all together, it’s a snap!”
–Dr. Steve Mouton, Northrop Grumman Proteomics, Texas
“We’re saving tons of money packing our own columns. It works great and it’s very user friendly.”
–Dr. Kimberly McKinney, Carolinas Medical Center, North Carolina
How it Works
Pressure Injection Cell Operation
Schematic of System with Pressure Injection Cell
Operationally, the pressure injection cell is analogous to a straw in a juice box. As you apply pressure in the box, the juice will be forced out through the straw. Likewise, the pressure injection cell is connected to pressurized gas, typically in a tank. A 0.5 to 2 mL tube containing the sample liquid is placed in the base of the pressure injection cell. A capillary is then placed through the ferrule in the cap and down into the sample tube. When packing a capillary, a frit assembly is placed at the distal end of the capillary to prevent particles from exiting and the entire pressure injection cell is typically placed on a stir plate. By regulating the gas pressure, you can adjust the flow rate of the sample into the capillary.
On the left is a LC/MS chromatogram of a peptide mixture prepared from a tryptic digested gel band, separated by a 5 µm C18 resin packed column (100 µm inside diameter by 10 cm long capillary). The Pressure Injection Cell was used to pack the LC/MS capillary column and to inject the sample into the mass spectrometer. Courtesy of Dr. Qishan Lin.
- Easy sample tube removal since the tube protrudes above the bottom section of the pressure injection cell.
- O-ring seal at interface ensures no leakage.
- Includes a 3-way valve for easy switching between pressurization and release of pressure.
- Three bolt system secures the cell, allows easy addition and removal of tubes / vials.
- Holds 0.5 to 2 ml microcentrifuge/Eppendorf tubes and 12 x 32 mm (glass) vials.
- Includes operator’s manual; 10 reusable ferrules for typical (380 µm outside diameter) capillaries (other ferrules are available); and 1.5 mL of Kasil and 0.5 mL of formamide and a cleaving tool for making your own frits.
- Warranty: 2 years parts and labor.
- 30 day money back guarantee.
- Made in USA.
|Pressure Rating||2500 psi (17,000 kPa)||8500 psi (58,000 kPa)||1000 psi (6,900 kPa)|
|Model with integrated magnetic stirplate||PC77-MAG||PC8500-MAG||PC1000-MAG|
|Distinguishing Benefit||Most Popular||Highest Pressure||Transparent|
Capillary loader for packing LC/MS columns and loading MS with integrated stir plate. 2500 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules and stir bar.
Capillary loader for packing LC/MS columns and loading MS. 8500 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules
Capillary loader for packing LC/MS columns and loading MS with integrated stir plate. 8500 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules and stir bar.
Capillary loader with transparent body for packing LC/MS columns and loading MS with integrated stir plate. 1000 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules
Capillary loader with transparent body for packing LC/MS columns and loading MS. 1000 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules
Capillary loader for packing LC/MS columns and loading MS. 2500 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules
Pressure Injection Cell Accessories
Adapter for connecting 1/8 inch stainless steel tubing to pressure regulators with metric fittings.
1/8 inch OD stainless steel tubing. Coil of 10 feet.
Adapter to connect 3 mm stainless steel tubing to pressure injection cells.
Ceramic tool for cleaving fused silica capillary.
Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.
Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.
Capillary for LC/MS columns, 75 um ID, 363 um OD, 10 meter length. Includes a cleaving tool.
High pressure regulator for use with PC77 pressure injection cells.
5×2 mm Teflon® coated magnetic stirbar.
PTFE Ferrules are used in the pressure cell to hold capillaries during packing. 0.4mm ID. Pack of 10.
Replacement O-ring for pressure cell body. Pack of 5.
Requirements and Other Information
What accessories do I need to use Next Advance Pressure Injection Cells?
In order to use a Next Advance Pressure Injection Cell you will need:
- A tank of pressurized gas (e.g. helium, nitrogen or argon) from a local supplier
- A pressure regulator (available alone or in a PACK-KIT)
- 1/8th inch stainless steel tubing from the regulator to the Pressure Injection Cell (available alone or in a PACK-KIT)
To pack capillaries, you will need:
- Capillary (available alone or in a PACK-KIT)
- Cleaving tool (available alone or part of FRIT-KIT which is included with the Pressure Injection Cell)
- Packing material
- Stir bar (available alone or in a PACK-KIT)
- FRIT KIT (included with the Pressure Injection Cell)
What if I live in a metric country and I want to use my own stainless steel tubing?
You will need to purchase ADPT-3mm1/8 which mounts to the Pressure Injection Cell and accepts 3mm outside diameter tubing.
What if I live in a metric country and I want to use TBNG10 (1/8th inch tubing) and a pressure regulator from my own country?
You will need to purchase ADPT-ISO which threads into ¼ ISO fitting (metric) on the pressure regulator and accepts 1/8th inch tubing.
What if I live in a metric country and I want to purchase the PACK-KIT?
You will need an adapter between their pressurized gas tank and the pressure regulator regulator (HPREG). Every country has its own standards for the fittings on gas tanks, and in some countries it’s not even standardized. Customers should tell their local gas tank supplier that our regulators come with a CGA 580 fitting mounted in a 1/4 NPT threaded hole and the supplier can supply the correct fitting or adapter.
Does the Pressure Injection Cell require electrical power?
No. The liquid sample is forced through the capillary using pressurized gas typically supplied by a tank Models with an integrated magnetic stirplate require electricity. They use a small power supply that plugs into a wall outlet. We supply the correct plug for your country.
Which gases can it use?
The choice of gas is not critical. Most customers use inert gases such as helium, nitrogen or argon. Dry air is fine too – if you purchase an adapter, NIP-AIR, to interface the tank with the pressure regulator.
Which adapters do I need?
If you’re operating the pressure injection cell with inert gas in the United States or other country using English parts and CGA fittings (580 for inert gases), you do not need any adapters. If you will use metric stainless steel tubing, you will need an adapter, ADPT-3mm1/8 to mate the 3 mm tubing with the 1/8 inch fitting on the pressure injection cell. This can be factory installed or installed in the field. If you will use 1/8 inch stainless tubing with a regulator with metric or ISO fittings, you will need the adapter, ADPT-ISOto1/8.
To use our regulator (HPREG) outside of the United States, you may need to purchase an adapter from your local gas supplier; they will know which fitting you will need to mate with our CGA 580 fitting. With multiple standard fittings in many countries and so many different standard fittings, we cannot be certain which fitting you will need.
I want to fill my capillaries with a certain solvent. How do I know if it is compatible with the components of the pressure cell?
The pressure cell should be compatible with most solvents. In theory, the solvent will only contact your sample tube and the capillary.
Here is a list of other components that may come in contact with the solvent.
- The shiny, hexagonal metal base and top are nickel plated
- The black base plate is anodized aluminum
- The bolts, valve, fittings, and nuts are stainless steel
- The plastic ferrule that clamps around your capillary is PTFE (Teflon)
How much pressure is required?
Loading samples into a capillary for mass spectroscopy typically requires 100 to 400 psi. Packing capillary columns typically requires 500 to 1000 psi.
Do I need a special pressure regulator?
Most pressure regulators for gas cylinders have a maximum working pressure that is too low for packing capillary columns. We sell a higher pressure regulator, model HPREG, that has a working pressure up to 1500 psi, which is ideal for packing standard length capillary columns.
How can you determine the approximate flow rate through a capillary?
If the solution is flowing only through an otherwise empty capillary tube, the flow rate is straightforward to calculate. However, a frit or a packed capillary typically causes much more flow resistance, so it is best to measure the flow rate with a test solution.
To measure the flow rate, run a test solution through a sample capillary and at the outlet place a calibrated micropipette. The solution should wick up the micropipette on its own so you don’t need any special fittings to seal them together. For example, Drummond and other brands of micropipettes, available from most laboratory product suppliers, come in 1, 2, 3, 4, 5, 10, 20, … µl volumes. Using a stopwatch, measure how long it takes from when the solution starts to exit the capillary and begins filling the micropipette until the micropipette is filled.
How do I use the FRIT-KIT?
For information on how to make a frit, please see the user manual.
Can the pressure cell be used to extract compounds using supercritical CO2?
The pressure cell can be adapted for this purpose. Please contact Next Advance technical support for additional information.
This video shows you how to set up and use the Pressure Injection Cells
Want a tutorial on how to pack a capillary column? Watch this great explanation at Benchfly by Dr. Charlie Knutson at MIT.
Researchers all over the world use our pressure cells to pack their own columns.