TPP Tissue Culture Products

Overview

TPP®, the premium tissue culture brand.

TPP bioreactor for cell culture At Next Advance, our goal is to bring you innovative, affordable, high-quality products for the biology laboratory that offer unique features over standard devices. That is why we proudly offer selections from the TPP® family of products. TPP® is a producer of high quality plastic disposable tools for tissue culture applications. Internationally recognized for unequaled quality, TPP®’s innovative designs significantly improve consistency and reproducibility in data generation.

Why take chances? How can you be sure that your culture tubes or bioreactors are not affecting your cultures? TPP® products are made without plasticizers, which could leach into your cultures. They are made without mold release agents or other additives commonly used in the manufacture of molded plastic parts. TPP® products are, however, made of virgin plastics of highest purity and quality, that meet the requirements of Pharmacopoeia (USP) Class 6 and Class 1 medical devices per directive 93/42. See the Next Advance TPP Tissue and Cell Culture Brochure

Suspension Cultures

TPP® Suspension Culture Vessels

alt= TPP® TubeSpin Bioreactors are ideal for high throughput screening and optimization of suspension cell cultures. Each bioreactor cap has a built-in 0.22μm hydrophobic membrane to maintain sterility and facilitate gas exchange. Growth conditions can be finely controlled by means of cap holes that can be selectively sealed for optimal gas transfer. The simple design containes no internal mixing or stirring mechanism which minimizes cell shearing and foam formation, and agitation is provided by rocking or shaking in an incubator. In this manner, hundreds of cell cultivations can be performed quickly and efficiently in parallel. TubeSpin Bioreactors are also ideally suited for the culture of bacteria, yeast, and other microbiological organisms in suspension. The simple, disposable format offers convenience and time-savings over traditional glass vessels such as test tubes which must be washed and autoclaved before each use. Both aerobic and anaerobic cultures can be grown by selectively covering the holes in the 0.22μm filter cap, offering the flexibility to perform mini plasmid preps, small-scale candidate screens, or other down-stream applications.

  • Entire Bioreactor can be centrifuged- no transfer necessary
  • Maximizes protein yields
  • Optimal gas exchange – cap holes can be selectively sealed
  • 0.22μm hydrophobic cap membrane minimizes evaporation and prevents contamination
  • Rapid sampling following centrifugation – entire tube can be sacrificed
  • No foam formation at any speed – no internal stirring mechanism
  • Sterile, certified nuclease, nucleic acid, and pyrogen-free
  • Made of pure, virgin polypropylene, without plasticizers or mold release agents.
tubespin-cap

5 cap holes of differing size can be selectively sealed to optimize growth conditions in the 50ml Bioreactor

bioreactor cell culture tissue culture bacteria yeast protein generation bottle roller LB broth Lysogeny broth HEPES

The Stabilizer container rack holds four 600ml TubeSpin Bioreactors on the Infinity Rocker Pro



Adherent Cells

TPP® Adherent Culture Vessels

T25 tissue culture flask confluent culture ES cells MEF cells feeder layer stem cells fibroblasts primary culture subcultivation ratio growth media
  • Observe cell growth for ideal harvest time
  • Detach Cells
  • Wash
  • Resuspend at desired density
  • Harvest
  • ….all in the same vessel!
The innovative design on the 10cm2 Tissue Culture flask incorporates the features of a tissue culture flask and a 50ml conical centrifuge tube into a single multi-functional culture vessel. Integration of these functions prevents cell loss and the possibility of contamination during transfer since this step is now unnecessary.
  • 10cm2 growth area – great for bringing up cells from frozen storage or for culturing primary cell lines
  • Fits standard 50ml tube rotor – can be spun up to 1,200 x g
  • The drop-shaped growth surface can be exactly worked with a cell scraper
  • Flat viewing window with excellent optical clarity for microscopy
  • White graduations and marking area
  • 0.22μm hydrophobic filter cap allows optimal gas exchange and prevents evaporation and contamination
  • Sterile, certified nuclease, nucleic acid, and pyrogen free
  • Made of pure, virgin plastic, without any plasticizers or mold release agents.
  • Will not fall through a microscope stage like a 35mm dish

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