Pressure Injection Cells

Overview

Get Better LC/MS Results and Save Money Too!
Pack your own LC/MS capillary columns.

See our capillary column packing kits.

Load your samples into mass spectrometers without transfer loss or contact with metallic surfaces.

The Pressure Injection Cell, sometimes called a “bomb loader” enables controlled dispensing of small-volume liquid samples. Using high pressure, the pressure injection cell has proved invaluable for two applications: densely packing nanobore capillary columns with solid-phase particles; and precisely infusing microliter samples directly from microcentrifuge tubes into mass spectrometers without additional transfers, wasted sample, or contact with metallic surfaces which adsorb some negatively charged molecules such as peptides with phospho or carboxy groups.

The Pressure Injection Cell holds 1 mL and 2 mL micro-centrifuge tubes as well as 12 x 32 mm glass vials in its central chamber (as shown at right). The assembly can be used on a typical magnetic stirrer, e.g. to keep particles in suspension. The Pressure Injection Cells accept various sample tubes, including 0.5 mL, 1.5 mL, and 2.0 mL microcentrifuge tubes and 12 mm diameter glass vials. The cell needs to be connected to a bottle/tank of compressed gas, such as Helium or Nitrogen. Packing capillary columns requires several hundred to about 1000 psi or sometimes more, and mass spectrometer injections typically require a few hundred psi. This unit is rated for a 2500 psi. 8500 psi units are available.

Pressure Injection Cells for packing capillaries

Why pay hundreds of dollars per packed capillary column for LC/MS? You can pack the columns, of various internal diameters, yourself. The Pressure Injection Cell comes with instructions on how to pack columns and is available as a stand alone unit (with 9 extra ferrules), or as a complete kit with ferrules, 1/8″ stainless steel tubing, a spool of capillary, a cleaving tool, and a frit assembly, or as a partial kit.

sample tubes used with injecting samples and packing capillaries

Accurate Results

The Pressure Injection Cell is made with the finest methods and components, thereby enabling you to acquire very accurate data. The high quality stainless steel fittings and valve are made by Swagelok. The body and cap of the pressure chamber are nickel coated. Special features not found elsewhere include a recess in the cap to allow easy access to the sample tube and a hexagonal shape to help align the cap to the body. The Pressure Injection Cell’s simple, rugged design and the use of top components ensure years of trouble free use and quality performance.

High Value

The simple and reliable product design, efficient manufacturing, internet marketing, and our low overhead enable us to sell the Pressure Injection Cells at a price almost 50% less than competitors charge. We don’t skimp on quality; we make the best product available. However, we do pass the savings along to our customers.

Risk Free

The Pressure Injection Cell comes with a 30 day money back guarantee and a 2 year warranty.


Download the Pressure Injection Cell brochure.


Testimonials

“We are very happy with the performance of our Next Advance pressure cell. Here are two representative total ion chromatograms generated from a capillary column using sub 2 micron, C18 particles and analyzed on a UPLC. As you can see the peak width is around 15 sec or less. We are getting this kind of resolution routinely from the columns packed by your pressure cell” Dr. Austin Yang, University of Maryland
“The pressure injection cell is really handy. I have two in my lab, one for packing capillaries and one for loading samples in my mass spec. Packing my own capillaries saves me hundreds of dollars on each one.” Dr. Qishan Lin, Director of the Proteomics Core Facility The Center for Functional Genomics, University at Albany, New York
“Hey, it works great. I am getting much better resolution because of packing my own columns and using the cell to directly inject my samples. The first purchase in a long time that was well worth the money. It is well made and so easy to use. After I purchased the fittings to tie it all together, it’s a snap!” Dr. Steve Mouton, Northrop Grumman Proteomics, Texas
“We’re saving tons of money packing our own columns. It works great and it’s very user friendly.” Dr. Kimberly McKinney, Carolinas Medical Center, North Carolina

How it Works

Pressure Injection Cell Operation

Operationally, the pressure injection cell is analogous to a straw in a juice box. As you apply pressure in the box, the juice will be forced out through the straw. Likewise, the pressure injection cell is connected to pressurized gas, typically in a tank. A 0.5 to 2 mL tube containing the sample liquid is placed in the base of the pressure injection cell. A capillary is then placed through the ferrule in the cap and down into the sample tube. When packing a capillary, a frit assembly is placed at the distal end of the capillary to prevent particles from exiting and the entire pressure injection cell is typically placed on a stir plate. By regulating the gas pressure, you can adjust the flow rate of the sample into the capillary.

spectrometer data

Sample Data

On the left is a LC/MS chromatogram of a peptide mixture prepared from a tryptic digested gel band, separated by a 5 µm C18 resin packed column (100 µm inside diameter by 10 cm long capillary). The Pressure Injection Cell was used to pack the LC/MS capillary column and to inject the sample into the mass spectrometer. Courtesy of Dr. Qishan Lin.

Above is a LC/MS chromatogram of a peptide mixture prepared from a tryptic digested gel band, separated by a 5 µm C18 resin packed column (100 µm inside diameter by 10 cm long capillary). The Pressure Injection Cell was used to pack the LC/MS capillary column and to inject the sample into the mass spectrometer. Courtesy of Dr. Qishan Lin.

diagram of pressure injection cell for packing LC/MS capillary columns

Schematic of System with Pressure Injection Cell

Features

Features

  • Easy sample tube removal since the tube protrudes above the bottom section of the pressure injection cell.
  • O-ring seal at interface ensures no leakage.
  • Includes a 3-way valve for easy switching between pressurization and release of pressure.
  • Three bolt system secures the cell, allows easy addition and removal of tubes / vials.
  • Holds 0.5 to 2 ml microcentrifuge/Eppendorf tubes and 12 x 32 mm (glass) vials.
  • Includes operator’s manual; 10 reusable ferrules for typical (380 µm outside diameter) capillaries (other ferrules are available); and 1.5 mL of Kasil and 0.5 mL of formamide and a cleaving tool for making your own frits.
  • Warranty: 2 years parts and labor.
  • 30 day money back guarantee.
  • Made in USA.

Models

Accessories

FAQ

Requirements

What accessories do I need to use Next Advance Pressure Injection Cells?

In order to use a Next Advance Pressure Injection Cell you will need:

To pack capillaries, you will need:

What if I live in a metric country and I want to use my own stainless steel tubing?

You will need to purchase ADPT-3mm1/8 which mounts to the Pressure Injection Cell and accepts 3mm outside diameter tubing.

What if I live in a metric country and I want to use TBNG10 (1/8th inch tubing) and a pressure regulator from my own country?

You will need to purchase ADPT-ISO which threads into ¼ ISO fitting (metric) on the pressure regulator and accepts 1/8th inch tubing.

What if I live in a metric country and I want to purchase the PACK-KIT?

You will need an adapter between their pressurized gas tank and the pressure regulator regulator (HPREG). Every country has its own standards for the fittings on gas tanks, and in some countries it’s not even standardized. Customers should tell their local gas tank supplier that our regulators come with a CGA 580 fitting mounted in a 1/4 NPT threaded hole and the supplier can supply the correct fitting or adapter.

capillary packing and sample injection for mass spectroscopy setup

Specifications

Does the Pressure Injection Cell require electrical power?

No. The liquid sample is forced through the capillary using pressurized gas typically supplied by a tank. Models with an integrated magnetic stirplate require electricity. They use a small power supply that plugs into a wall outlet. We supply the correct plug for your country.

Which gases can it use?

The choice of gas is not critical. Most customers use inert gases such as helium, nitrogen or argon. Dry air is fine too – if you purchase an adapter, NIP-AIR, to interface the tank with the pressure regulator.

Which adapters do I need?

If you’re operating the pressure injection cell with inert gas in the United States or other country using English parts and CGA fittings (580 for inert gases), you do not need any adapters. If you will use metric stainless steel tubing, you will need an adapter, ADPT-3mm1/8 to mate the 3 mm tubing with the 1/8 inch fitting on the pressure injection cell. This can be factory installed or installed in the field. If you will use 1/8 inch stainless tubing with a regulator with metric or ISO fittings, you will need the adapter, ADPT-ISOto1/8.

To use our regulator (HPREG) outside of the United States, you may need to purchase an adapter from your local gas supplier; they will know which fitting you will need to mate with our CGA 580 fitting. With multiple standard fittings in many countries and so many different standard fittings, we cannot be certain which fitting you will need.

I want to fill my capillaries with a certain solvent. How do I know if it is compatible with the components of the pressure cell?

The pressure cell should be compatible with most solvents. In theory, the solvent will only contact your sample tube and the capillary.

Here is a list of other components that may come in contact with the solvent.

  • The shiny, hexagonal metal base and top are nickel plated
  • The black base plate is anodized aluminum
  • The bolts, valve, fittings, and nuts are stainless steel
  • The plastic ferrule that clamps around your capillary is PTFE (Teflon)

Operation

Learn more about pressure cell operation

How much pressure is required?

Loading samples into a capillary for mass spectroscopy typically requires 100 to 400 psi. Packing capillary columns typically requires 500 to 1000 psi.

Do I need a special pressure regulator?

Most pressure regulators for gas cylinders have a maximum working pressure that is too low for packing capillary columns. We sell a higher pressure regulator, model HPREG, that has a working pressure up to 1500 psi, which is ideal for packing standard length capillary columns.

How can you determine the approximate flow rate through a capillary?

If the solution is flowing only through an otherwise empty capillary tube, the flow rate is straightforward to calculate. However, a frit or a packed capillary typically causes much more flow resistance, so it is best to measure the flow rate with a test solution.

To measure the flow rate, run a test solution through a sample capillary and at the outlet place a calibrated micropipette. The solution should wick up the micropipette on its own so you don’t need any special fittings to seal them together. For example, Drummond and other brands of micropipettes, available from most laboratory product suppliers, come in 1, 2, 3, 4, 5, 10, 20, … µl volumes. Using a stopwatch, measure how long it takes from when the solution starts to exit the capillary and begins filling the micropipette until the micropipette is filled.

How do I use the FRIT-KIT?

For information on how to make a frit, please see the user manual.

Can the pressure cell be used to extract compounds using supercritical CO2?

The pressure cell can be adapted for this purpose. Please contact Next Advance technical support for additional information.

Tutorial

This video shows you how to set up and use the Pressure Injection Cells

 

cap_pack_small

Want a tutorial on how to pack a capillary column? Watch this great explanation at Benchfly by Dr. Charlie Knutson at MIT.

Publications

Researchers all over the world use our pressure cells to pack their own columns.

Valdés, A., García-Cañas, V., Artemenko, K. A., Simo, C., Bergquist, J., & Cifuentes, A. (2016). Nano-liquid chromatography-orbitrap MS -based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells. Molecular & Cellular Proteomics, mcp.M116.061481. https://doi.org/10.1074/mcp.M116.061481
Musunuri, S., Khoonsari, P. E., Mikus, M., Wetterhall, M., Häggmark-Mänberg, A., Lannfelt, L., … Kultima, K. (2016). Increased Levels of Extracellular Microvesicle Markers and Decreased Levels of Endocytic/Exocytic Proteins in the Alzheimer’s Disease Brain. Journal of Alzheimer’s Disease, 54(4), 1671–1686. https://doi.org/10.3233/JAD-160271
Khoonsari, P. E., Häggmark, A., Lönnberg, M., Mikus, M., Kilander, L., Lannfelt, L., … Shevchenko, G. (2016). Analysis of the Cerebrospinal Fluid Proteome in Alzheimer’s Disease. PLOS ONE, 11(3), e0150672. https://doi.org/10.1371/journal.pone.0150672
Warnecke, A., Musunuri, S., N’diaye, M., Sandalova, T., Achour, A., Bergquist, J., & Harris, R. A. (2016). Nitration of MOG diminishes its encephalitogenicity depending on MHC haplotype. Journal of Neuroimmunology. https://doi.org/10.1016/j.jneuroim.2016.11.008
Sargsyan, E., Artemenko, K., Manukyan, L., Bergquist, J., & Bergsten, P. (2016). Oleate protects beta-cells from the toxic effect of palmitate by activating pro-survival pathways of the ER stress response. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1861(9), 1151–1160. https://doi.org/10.1016/j.bbalip.2016.06.012
Ross, E. E., Hoag, C., Pfeifer, Z., Lundeen, C., & Owens, S. (2016). Metal ion binding to phospholipid bilayers evaluated by microaffinity chromatography. Journal of Chromatography A, 1451, 75–82. https://doi.org/10.1016/j.chroma.2016.05.012
Engholm-Keller, K., & Larsen, M. R. (2016). Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation. In L. von Stechow (Ed.), Phospho-Proteomics (Vol. 1355, pp. 161–177). New York, NY: Springer New York. Retrieved from http://link.springer.com/10.1007/978-1-4939-3049-4_11
Richards, A. L., Hebert, A. S., Ulbrich, A., Bailey, D. J., Coughlin, E. E., Westphall, M. S., & Coon, J. J. (2015). One-hour proteome analysis in yeast. Nature Protocols, 10(5), 701–714. https://doi.org/10.1038/nprot.2015.040
Schey, K. L., Luther, J. M., & Rose, K. L. (2015). Proteomics characterization of exosome cargo. Methods. https://doi.org/10.1016/j.ymeth.2015.03.018
Musunuri, S., Kultima, K., Richard, B. C., Ingelsson, M., Lannfelt, L., Bergquist, J., & Shevchenko, G. (2015). Micellar extraction possesses a new advantage for the analysis of Alzheimer’s disease brain proteome. Analytical and Bioanalytical Chemistry, 407(4), 1041–1057. https://doi.org/10.1007/s00216-014-8320-8
Elf, K., Shevchenko, G., Nygren, I., Larsson, L., Bergquist, J., Askmark, H., & Artemenko, K. (2014). Alterations in muscle proteome of patients diagnosed with amyotrophic lateral sclerosis. Journal of Proteomics, 108, 55–64. https://doi.org/10.1016/j.jprot.2014.05.004
Levy, M. H., Goswami, S., Plawsky, J., & Cramer, S. M. (2013). Parameters Governing the Formation of Photopolymerized Silica Sol–Gel Monoliths in PDMS Microfluidic Chips. Chromatographia, 76(15–16), 993–1002. https://doi.org/10.1007/s10337-013-2493-8
Levy, M. H., Plawsky, J., & Cramer, S. M. (2013). Photopolymerized sol-gel monoliths for separations of glycosylated proteins and peptides in microfluidic chips: Other Techniques. Journal of Separation Science, 36(14), 2358–2365. https://doi.org/10.1002/jssc.201200990
Sjödin, M. O. D., Wetterhall, M., Kultima, K., & Artemenko, K. (2013). Comparative study of label and label-free techniques using shotgun proteomics for relative protein quantification. Journal of Chromatography B, 928, 83–92. https://doi.org/10.1016/j.jchromb.2013.03.027
Shevchenko, G., Wetterhall, M., Bergquist, J., Höglund, K., Andersson, L. I., & Kultima, K. (2012). Longitudinal Characterization of the Brain Proteomes for the Tg2576 Amyloid Mouse Model Using Shotgun Based Mass Spectrometry. Journal of Proteome Research, 121030111932006. https://doi.org/10.1021/pr300808h
Liao, Z., Wan, Y., Thomas, S. N., & Yang, A. J. (2012). IsoQuant: A Software Tool for Stable Isotope Labeling by Amino Acids in Cell Culture-Based Mass Spectrometry Quantitation. Analytical Chemistry, 84(10), 4535–4543. https://doi.org/10.1021/ac300510t
Martin, B., Chadwick, W., Yi, T., Park, S.-S., Lu, D., Ni, B., … Maudsley, S. (2012). VENNTURE–A Novel Venn Diagram Investigational Tool for Multiple Pharmacological Dataset Analysis. PLoS ONE, 7(5), e36911. https://doi.org/10.1371/journal.pone.0036911
Thomas, S. N., Wan, Y., Liao, Z., Hanson, P. I., & Yang, A. J. (2011). Stable Isotope Labeling with Amino Acids in Cell Culture Based Mass Spectrometry Approach to Detect Transient Protein Interactions Using Substrate Trapping. Analytical Chemistry, 83(14), 5511–5518. https://doi.org/10.1021/ac200950k
Park, S.-S., & Maudsley, S. (2011). Discontinuous pH gradient-mediated separation of TiO2-enriched phosphopeptides. Analytical Biochemistry, 409(1), 81–88. https://doi.org/10.1016/j.ab.2010.10.003
Lu, Q., & Collins, G. E. (2010). Lab on a chip packing of submicron particles for high performance EOF pumping. Journal of Chromatography A, 1217(45), 7153–7157. https://doi.org/10.1016/j.chroma.2010.09.009
Luo, Y., Yang, C., Jin, C., Xie, R., Wang, F., & McKeehan, W. L. (2009). Novel phosphotyrosine targets of FGFR2IIIb signaling. Cellular Signalling, 21(9), 1370–1378. https://doi.org/10.1016/j.cellsig.2009.04.004
Lu, Q., & Collins, G. E. (2009). A fritless, EOF microchip pump for high pressure pumping of aqueous and organic solvents. Lab on a Chip, 9(7), 954. https://doi.org/10.1039/b816291c
Lohrig, K., & Wolters, D. (2009). Multidimensional Protein Identification Technology. In J. Reinders & A. Sickmann (Eds.), Proteomics (Vol. 564, pp. 143–153). Totowa, NJ: Humana Press. Retrieved from http://link.springer.com/10.1007/978-1-60761-157-8_8
Borowsky, J. F., Giordano, B. C., Lu, Q., Terray, A., & Collins, G. E. (2008). Electroosmotic Flow-Based Pump for Liquid Chromatography on a Planar Microchip. Analytical Chemistry, 80(21), 8287–8292. https://doi.org/10.1021/ac801497r

FAQs

Pressure Cell FAQs

Requirements

What accessories do I need to use Next Advance Pressure Injection Cells?

In order to use a Next Advance Pressure Injection Cell you will need:

To pack capillaries, you will need:

What if I live in a metric country and I want to use my own stainless steel tubing?

You will need to purchase ADPT-3mm1/8 which mounts to the Pressure Injection Cell and accepts 3mm outside diameter tubing.

What if I live in a metric country and I want to use TBNG10 (1/8th inch tubing) and a pressure regulator from my own country?

You will need to purchase ADPT-ISO which threads into ¼ ISO fitting (metric) on the pressure regulator and accepts 1/8th inch tubing.

What if I live in a metric country and I want to purchase the PACK-KIT?

You will need an adapter between their pressurized gas tank and the pressure regulator regulator (HPREG). Every country has its own standards for the fittings on gas tanks, and in some countries it’s not even standardized. Customers should tell their local gas tank supplier that our regulators come with a CGA 580 fitting mounted in a 1/4 NPT threaded hole and the supplier can supply the correct fitting or adapter.

capillary packing and sample injection for mass spectroscopy setup

Specifications

Does the Pressure Injection Cell require electrical power?

No. The liquid sample is forced through the capillary using pressurized gas typically supplied by a tank. Models with an integrated magnetic stirplate require electricity. They use a small power supply that plugs into a wall outlet. We supply the correct plug for your country.

Which gases can it use?

The choice of gas is not critical. Most customers use inert gases such as helium, nitrogen or argon. Dry air is fine too – if you purchase an adapter, NIP-AIR, to interface the tank with the pressure regulator.

Which adapters do I need?

If you’re operating the pressure injection cell with inert gas in the United States or other country using English parts and CGA fittings (580 for inert gases), you do not need any adapters. If you will use metric stainless steel tubing, you will need an adapter, ADPT-3mm1/8 to mate the 3 mm tubing with the 1/8 inch fitting on the pressure injection cell. This can be factory installed or installed in the field. If you will use 1/8 inch stainless tubing with a regulator with metric or ISO fittings, you will need the adapter, ADPT-ISOto1/8.

To use our regulator (HPREG) outside of the United States, you may need to purchase an adapter from your local gas supplier; they will know which fitting you will need to mate with our CGA 580 fitting. With multiple standard fittings in many countries and so many different standard fittings, we cannot be certain which fitting you will need.

I want to fill my capillaries with a certain solvent. How do I know if it is compatible with the components of the pressure cell?

The pressure cell should be compatible with most solvents. In theory, the solvent will only contact your sample tube and the capillary.

Here is a list of other components that may come in contact with the solvent.

  • The shiny, hexagonal metal base and top are nickel plated
  • The black base plate is anodized aluminum
  • The bolts, valve, fittings, and nuts are stainless steel
  • The plastic ferrule that clamps around your capillary is PTFE (Teflon)

Operation

Learn more about pressure cell operation

How much pressure is required?

Loading samples into a capillary for mass spectroscopy typically requires 100 to 400 psi. Packing capillary columns typically requires 500 to 1000 psi.

Do I need a special pressure regulator?

Most pressure regulators for gas cylinders have a maximum working pressure that is too low for packing capillary columns. We sell a higher pressure regulator, model HPREG, that has a working pressure up to 1500 psi, which is ideal for packing standard length capillary columns.

How can you determine the approximate flow rate through a capillary?

If the solution is flowing only through an otherwise empty capillary tube, the flow rate is straightforward to calculate. However, a frit or a packed capillary typically causes much more flow resistance, so it is best to measure the flow rate with a test solution.

To measure the flow rate, run a test solution through a sample capillary and at the outlet place a calibrated micropipette. The solution should wick up the micropipette on its own so you don’t need any special fittings to seal them together. For example, Drummond and other brands of micropipettes, available from most laboratory product suppliers, come in 1, 2, 3, 4, 5, 10, 20, … µl volumes. Using a stopwatch, measure how long it takes from when the solution starts to exit the capillary and begins filling the micropipette until the micropipette is filled.

How do I use the FRIT-KIT?

For information on how to make a frit, please see the user manual.

Can the pressure cell be used to extract compounds using supercritical CO2?

The pressure cell can be adapted for this purpose. Please contact Next Advance technical support for additional information.

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