Quietek CO2 Induction Systems

Have You Heard? 2020 update to AVMA Guidelines

The AVMA has published the 2020 edition of the AVMA Guidelines for the Euthanasia of Animals. Per section 12.2 (and others), “the conditions for the use of CO2 with rodents in the laboratory have changed from a recommended 10% to 30% of the chamber or cage volume/min to a recommended 30% to 70% of the chamber or cage volume/min.”

If you are a current Quietek customer, no problem! Just email us at [email protected] for guidance on settings to use to ensure compliance with these regulations.

If you want to order a Quietek, no problem! We are revising software to meet the new guidelines and will process your order as soon as the updated equipment is fully tested and released for use.


Automated Euthanization Device Ensures AVMA Compliance

humane CO2 induction guidelines

Now you can ensure that your laboratory rodents are humanely euthanized without disrupting your laboratory workflow. The Quietek™, in use worldwide since 2013, is the original, affordable, fully-automated instrument specifically designed to ensure compliance with 2020 AVMA guidelines. (It is also designed to withstand urine and excrement.)

Ask us about a 30 day trial.

Takes the Guesswork Out of Euthanasia

Designed by animal research facility managers and scientists, easy to use, accurate system ensures that you correctly administer CO2 by automatically calculating gas delivery for your cage volume and desired fill rate. CO2 conditions will be consistent every time, thereby eliminating a source of variation.

Fail-Safe and Easy to Operate

All you need to do is press the start button. There are no valves to turn (or accidentally leave open) between runs and no regulators to adjust. Ideal for the inexperienced, sleep deprived, or overworked laboratory technician. The Quietek also features a key lock that prevents unwanted or accidental tampering with the settings.

Reduces Impact on Your Lab

The Quietek seamlessly integrates into your current protocols. Use it with most common chambers or cages. Lids are available in several sizes.


humane CO2 induction in rodent cage

Easy Compliance

The Quietek seamlessly integrates into your existing workflow with no additional training required. Just push the “Start” button to operate.

Prevents Errors

The recommended gas rate is administered appropriately, every time. Use the key lock to prevent settings from being changed.


The Quietek eliminates variation in CO2 induction conditions which can change tissue characteristics. A run timer allows you to document conditions for each animal.


Flow and pressure sensors ensure that the flow of CO2 is accurate, so that ethical standards are maintained. With this precision, you can tailor the flow rate as desired within the guidelines, such as to be more gradual or to save time.

Download the Quietek brochure.

Typical Quietek setup

Quietek CO2 induction euthanasia system setup

No animals were harmed or euthanized for the main purpose of developing or testing this product. The AVMA has not certified this product



Both the Quietek 1 and Quietek XL models can switch between 2 cage sizes, for example, if a lab works with mice and rats or 2 different size mouse cages.
Quietek features for humane CO2 induction Quietek setup

  • Gas flow rate calculated automatically for your cage size
  • Key lockout prevents unauthorized tampering with controls
  • Consistent gas delivery
  • Run timer for ease of documentation
  • Works with common rodent chambers or cages
  • Lids available in several sizes
  • Includes quick release fitting and hoses to connect to chamber or cage lid
  • Adapters available to fit your current cage systems. Please contact us.
  • CE Mark
  • Made in the USA


Quietek 1
Quietek XL
Quietek 2
One-touch operation. Just press “Start” Button. check check check
Key lockout to prevent tampering with controls check check check
Adjustable flow rate from 30 – 70% chamber volume/min. Custom flow rates available check check check
For the euthanasia of mice, rats, hamsters, guinea pigs, gerbils, degus and cotton rats check check check
Cage or chamber size to be controlled 1 cage, 2 – 25 L 1 cage, 12.5 – 140 L 2 cages, 2 – 25 L
Dual settings for two different cage or chamber volumes check check
Has CE Mark check check check
Dual port system to 2 separate chambers check

All of our models can switch between 2 cage/chamber sizes, for example, if a lab works with mice and rats or 2 different size mouse cages.

Contact us about custom models.

Quietek models are designed for different sizes and numbers of cages. The Quietek 1 is suitable for cages between 2 and 25 liters in volume, and can be used with one chamber at a time. For larger chamber sizes (up to 140 liters), we offer the Quietek XL.

Not sure which model to choose? Try our handy Cage Volume Calculator.

Cage Lids

humane CO2 induction rodent cage

Cage Lids for use with the Quietek™

We offer a line of cage lids that fit almost all commercially available cages. The Quietek controller can also be used with other manufacturers’ lids.

Contact us if you need a lid that is not listed here.


humane CO2 induction rodent cage

Quietek™ Accessories

Accessories include spare keys and replacement tubing in the color of your choice.

If you need additional fittings, other tubing, or anything else, please contact us.

Cage Lids for use with the Quietek

We offer a line of cage lids that fit almost all commercially available cages. The Quietek controller can also be used with other manufacturers’ lids.

Contact us if you need a lid that is not listed here.


Calibration & Service

The Quietek™ is a robust instrument. Its precision should not vary significantly over time, however, periodic calibration ensures proper operation such that it meets or exceeds the ethical and quality standards of your animal use program. The Quietek is not serviceable by anyone other than Next Advance certified technicians. The software is integrally coupled to the hardware. Modification of any components may render the instrument inaccurate and will void any warranty.

Next Advance continuously strives to provide industry leading solutions to researchers in the life sciences. We regularly identify and make modifications to our products to improve functionality and operation. The Quietek calibration service will provide you with the latest software update, will include repair and replacement of worn parts (misuse or abuse is not covered), and will submit your Quietek to our vigorous testing procedure, thereby ensuring that the Quietek is fully compliant when it leaves our facility.

Any custom program requirements you wish to have can likely be included as well. If you have custom flow requirements we can incorporate those as part of this service.

Note that for calibration or any other service, you must clean your Quietek and certify that it has been decontaminated. A step-by-step guide for getting an instrument calibrated can be found here. Please contact us before shipping the Quietek or if you have any questions.

Guidelines By Country

International Guidelines

Now you can ensure that your laboratory rodents are humanely euthanized without disrupting your laboratory workflow. Introducing the Quietek™, the affordable, fully-automated device specifically designed by us to ensure compliance with strict international guidelines.

United States: “An optimal flow rate for CO2 euthanasia systems should displace 30% to 70% of the chamber or cage volume/min. Prefilled chambers are unacceptable.” 2020 AVMA Guidelines (pg. 49)

Canada: “…a gradual-fill rate of less than 30% and greater than 20% of the chamber volume per minute should be used.” CCAC Guidelines on: Euthanasia of Animals Used in Science (pg. 19)

United Kingdom: “Exposure to carbon dioxide gas in a rising concentration: appropriate for rodents and rabbits and birds up to 1.5kg…a controllable, metered source of carbon dioxide gas should be used.” The Humane Killing of Animals under Schedule 1 to the Animals (Scientific Procedures) Act 1986 (pg. 6)

European Union: “Only to be used in gradual fill.” Official Journal of the European Union (pg. 72, 73)

Australia: “a gradual fill of the chamber with 100% CO2 at a rate to fill 20% of the chamber volume per minute was agreed as good practice” Guidelines to Promote the Well-being of Animals Used for Scientific Purposes (pg. H3)


Quietek FAQs

Learn more about laboratory rodent euthanasia

What will I need to use the Quietek™ in my facility?

The Quietek requires a CO2 source with a mimimum pressure of 25 psi. We do not recommend pressures above 60 psi. Both tanks and institutional gas supply lines are acceptable. You will also require a solid-sided cage and a lid such as the Quietek lid.

Can I use other cage lids or chambers?

The Quietek controller can be used with other lids and with chambers. To connect the Quietek to your chamber or lid, you can purchase tubing from us or use your own tubing. We can supply adapters to fit a wide range of lids and chambers. Call or email us for more information.

How much control do I have over the CO2 flow rate?

The flow rate is completely adjustable within the parameters set out in the AVMA regulations. To ensure proper compliance, it is also necessary to determine the cage volume.

What is the difference between models?

Any of our two models can switch between 2 cage sizes, if a lab works with mice and rats or 2 different size mouse cages. The Quietek 1 is available with and without RapidFill™, in which after the rodent is anesthetized, you can maximize the fill rate and save time. The Quietek XL is for larger cages.

How do I get the CO2 out of the induction chamber between euthanasia cycles?

Best practice is often considered to euthanize in the home cage as there is less stress to the animals (in this situation there is no need to purge the CO2). If you are using a euthanasia chamber or clean cage, then purging the chamber between uses is required (to maintain compliance). CO2 is heavier than “normal” air and so simply tipping the chamber over will release the CO2 from the cage. This process may take several minutes based on the size of your cage and air flow in the area.

How do I determine my cage volume?

Most rodent cages are at least somewhat tapered, or otherwise not perfectly rectangular in profile. Your institution may have specific guidelines for measuring cages; if so, you should follow those guidelines. If it does not, we suggest the following method:

If the cage is only very slightly tapered (e.g., Allentown cages), simply calculate the volume using the length and width (of the inside) at the top of the cage. Multiply the inside length, width and height of the cage, in cm. Subtract about 4 times the height and 2 times the length plus width, to account for the rounded corners, then divide by 1000 to get the volume in liters. (There are 1000 cubic centimeters in one liter.) For more strongly tapered cages, measure the length and width 1/2 way down from the top.

V = H[wl + 0.5(W-w)l + 0.5(L-l)w + (1/3)(W-w)(L-l)]

If your cage is wedge-shaped (e.g., Animal Systems’ M.I.C.E cages) or otherwise irregular in outline, it is best to measure the cage volume directly by filling it with water.

A handy calculator to approximate the volume of a cuboid (box-shaped) cage is available.

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