BlotBot – Western Blot Processor

Throw out your timers! Don't babysit your blots again!

The Freedom Rocker™ BlotBot® is an automated blot processor for incubating and washing blots and gels, after the transfer from gel to blot. Using a microprocessor for precise timing and dispensing, it automatically processes Westerns, Northerns, and Southerns in a consistent manner, every time. Ideal for unattended staining of gels, it frees you from babysitting your samples. Simply add your buffers and reagents, and the BlotBot carries out your existing protocol.

  • Consistent processing. Better results.
  • Plan your next experiment over coffee, not lunch.
  • Process blots unattended so you can focus on other tasks.
  • Use as little as 3.5 mL of antibody (or up to 50 mL of reagent) for each mini blot, and recover separately.
  • Large color LCD display for easy programming of protocols.

BlotBot vs Manual Western Blot

Automated Processor for Western Blots
Automated Processor for Western Blots
Consistent automated blot processing with the BlotBot.
western blot, processed / washed with Freedom Rocker
Western blot analysis of extracts from human squamous carcinoma cells using anti-myc antibody. Courtesy of Dr. Aparna Ranganathan, the University at Albany (SUNY).

Throw Out Your Timers

Are you chained to your timer? Throw it out and use the Freedom Rocker BlotBot. Simply place your blots, gels, or slides in trays, set the program, connect the bottles/tubes of reagents and walk away. The microcontroller in the Freedom Rocker BlotBot will ensure that the reagents and washes will be accurately pumped into and out of the trays at the precise times. Results will be consistent because all trays will receive the same reagent or wash buffer at the same time in equal volumes. Multi-dispense models can even dispense one different reagent to each tray, i.e. primary or secondary antibody.

Be productive and concentrate on another task, enjoy your lunch, OR let it run overnight!

Improve Consistency and Accuracy

By automating the processing of blots and gels, the Freedom Rocker BlotBot yields very consistent and accurate blots, gels, and slides. It automatically dispenses and aspirates equal volumes of reagents from multiple trays simultaneously. The samples in all the trays will experience the exact same protocol.

Shave Months Off Your Research

Complete the testing portion of your research project in half the time. Blots are ready when you arrive at work. You can review them over coffee, not lunch, so you can start your next test iteration the same day.

Increase Productivity

Automatically processing blots and gels cuts down on distractions. Instead of babysitting your blots you have more time to focus on other tasks, eat lunch, or attend meetings – or let it run overnight. Through increased productivity, the Freedom Rocker BlotBot pays for itself in about 30 uses.

The Freedom Rocker BlotBot replicates your existing protocols. There is no need to develop new protocols so you can start processing right away.

Risk Free Purchase

The Freedom Rocker BlotBot comes with a 30 day money back guarantee and a 2 year warranty. Customers have been using Freedom Rockers since 2005, and most of those sold in the first years remain in use today.

Download Additional Information

Skip the Repetitive Steps

Freedom Rocker Steps for western blot processing

Portion of a sample protocol – showing the addition and washing of one reagent using the Freedom Rocker BlotBot.

The Freedom Rocker BlotBot can be programmed to perform up to 3 of these reagent steps, and process your Western blots and gels unattended. Your blots can be ready to analyze when you arrive at work the following morning.

Automated Processor for Western Blots


“The BlotBot saved my life – not only does it save me a ton of time because I don’t have to babysit my blots while it’s processing, but the blots come out really clean and consistent every time.” Srividya Venkitachalam, Case Western Reserve University
"We are so excited about our new BlotBot that my students have asked me to acquire another one!" Dr. Karen Briski, University of Louisiana at Monroe
"The results from the BlotBot are higher quality and more consistent than manual blot processing. I love having the blots processed overnight, ready to image in the morning. Amazing instrument!" Nat Clark, University of Texas Health and Science Center at San Antonio
"Overall I am pretty impressed by the BlotBot, because so far the membranes came out clean and results are comparable to those generated by our own regular way." Rachel Pong, University of Texas Southwestern Medical School
"I'm one of the first users of the Freedom Rocker at Amgen in Cambridge, MA. The machine gets LOTS of use from all of us here." Dr. Barbara Grubinska, Amgen
"We love it! It saves us a lot of time." Dr. Lilliane Roellard, Genentech
"It is perfect for silver staining of gels for mass spectrometry. It has made an UNBELIEVABLE impact on data generation for me in cancer protein screening." Dr. Steve Griffiths, Atlantic Cancer Research Institute
"We are very pleased with the instrument." Dr. Richard Ingram, Schering-Plough
"The Freedom Rocker is really convenient." Dr. Michele Lastro, The UAlbany Gen*NY*Sis Center for Excellence in Cancer Genomics

BlotBot Models

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