Anti-Foam NOW controls excess frothing or foaming of samples during homogenization
| Mouse Skin (300 µL RIPA buffer) | Mouse Lungs (600 µL RIPA buffer) | |||
|---|---|---|---|---|
| Mouse tissues were homogenized in 300 µL (GREEN Lysis kit) or 600 µL (NAVY Lysis kit) of RIPA buffer with or without Anti-Foam NOW. Note the significant reduction in foaming in the presence of Anti-Foam NOW. |
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| Anti-Foam NOW | ✗ | ✓ | ✗ | ✓ |
Anti-Foam NOW is compatible with gDNA isolation from mouse tissues
| Mouse Kidney | Mouse Liver | |||
|---|---|---|---|---|
| Mouse Kidney and Liver samples were homogenized in the lysis buffer with or without Anti-Foam NOW. Genomic DNA was isolated from the lysates and analyzed for their integrity. Both OD260/280 and OD260/230 ratios were comparable between the samples. |
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| Anti-Foam NOW | ✗ | ✓ | ✗ | ✓ |
Anti-Foam NOW is compatible with protein downstream applications
| Mouse Brain samples were homogenized using PINK Lysis kit in 300 µL of RIPA buffer with or without Anti-Foam NOW. Lysates were processed for Western blotting and Coomassie blue staining. |
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(GAPDH) 39kDa | ||
| Anti-Foam NOW | ✗ | ✓ | ✗ | ✓ | |
