Anti-Foam NOW controls excess frothing or foaming of samples during homogenization
|Mouse Skin (300 µL RIPA buffer)||Mouse Lungs (600 µL RIPA buffer)|
|Mouse tissues were homogenized in 300 µL (GREEN Lysis kit) or 600 µL (NAVY Lysis kit) of RIPA
buffer with or without Anti-Foam NOW. Note the significant reduction in foaming in the presence of
Anti-Foam NOW is compatible with gDNA isolation from mouse tissues
|Mouse Kidney||Mouse Liver|
|Mouse Kidney and Liver samples were homogenized in the lysis buffer with or without Anti-Foam NOW.
Genomic DNA was isolated from the lysates and analyzed for their integrity. Both OD260/280 and OD260/230
ratios were comparable between the samples.
Anti-Foam NOW is compatible with protein downstream applications
|Mouse Brain samples were homogenized using PINK Lysis kit in 300 µL of RIPA buffer with or without Anti-Foam
NOW. Lysates were processed for Western blotting and Coomassie blue staining.