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Specifications
Operation
Troubleshooting
Specifications:
Can the
FREEDOM ROCKER™ work in labs in other countries?
Yes. A wall transformer will convert the voltage
from your wall outlet to 12V DC for the Freedom Rocker™.
Select the proper voltage when you order your instrument.
How much room does the
FREEDOM ROCKER™ require?
The
Freedom Rocker™
takes up only 10 inches (25 cm) of lab bench. It is
10 inches (25 cm) wide by 15 inches (38 cm) deep,
including the pump and the rocker. The bottles for
wash buffer, waste, and reagent collection can be placed
behind or to the side of the Freedom Rocker™,
or even on a shelf above or below the lab bench.
Can the
Freedom Rocker™
drain the waste to a sink?
Yes,
the hose for waste liquid can either run to a container or
to a sink.
What happens at the end of the final wash step?
After the programmed duration of the final wash step, the
instrument beeps to let the user know that the wash is
complete. However, to keep the membranes, gels, or slides
from drying out, the rocking continues and the wash buffer
is not pumped from the tray(s) until the user depresses
the “RUN”, the "PAUSE/CONTINUE" or the “DRAIN” button. Then the rocking
stops and the buffer is pumped out of the trays.
How many
trays can the
FREEDOM ROCKER™
run at once?
The
Freedom Rocker™
models FRSK22 and DX2 can run 1 large tray or 2 tray small
or medium trays. With the purchase of AHDS24 Small
Aspiration Head, one small or medim tray can be used.
The FRSK42 and the DX4 models run 4 trays at once.
To run just 1 or 2 trays at a time, a different tube set
and a different aspiration head are required. 2 tray
accessory kits are available. Or the aspiration heads and
the tube sets can be ordered separately. See the
pricelist for
ordering information and current pricing.
Do all the
trays follow the same protocol?
Yes, the Freedom Rocker™
pumps the liquids in and out of all the trays
simultaneously to/from the same reagent/waste containers.
Different reagents can be manually poured in the different
trays initially, however, when these reagents are
aspirated out of the trays, they will be combined in the
waste or recover bottle.
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Operation:
How do you change the settings?
Press the “SELECT” button several times, until the
parameter you want to change lights up. Then rotate the
knob to adjust the setting to the value you want. Then
press the “SELECT” button again if you want to change
another parameter.
How do you program whether or not the reagent solution is
recovered?
Pressing the “RECOVER REAGENT” button on the front panel
changes whether or not the reagent solution is collected
after the reagent step. When the light by the button is
on, the reagent will be recovered. If the light is off,
when the reagent solution is aspirated, it will be sent to
the waste container.
How do you operate in Manual Mode?
Simply press the “FILL”, “ROCK”, or “DRAIN” buttons to
dispense wash buffer, rock, or aspirate the liquid in the
tray(s). The Rock rate and the dispense/aspirate volumes
can be changed by pressing the “SELECT” button until the
desired parameter lights up, then rotate the knob to
adjust the setting. By pressing any of the “FILL”,
“ROCK”, or “DRAIN” buttons overrides the Automatic and
Semi-automatic modes, putting the instrument into Manual
Mode.
What is Semi-Automatic Mode?
In
semi-automatic mode the rocker operates as an ordinary
rocker with a built-in countdown timer. Simply set the
reagent duration to the desired time, set the wash
durations to “0”, and press “RUN”. The washes will not
occur. After the desired duration, the instrument will
beep. (The rocking will continue and the liquid will
remain in the trays so that the membrane, gel, or slides
do not dry out.) Press “RUN” or “DRAIN” to stop the
rocking and aspirate the liquid.)
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Troubleshooting
If nothing happens. All the lights are off and the display
is blank.
Check that the power is properly connected. If the wall
transformer is plugged in all the way and the power line
is plugged into the instrument, then either the wall
outlet or the wall transformer is bad. Plug something
else into the same wall outlet. Does it work? If so,
then the power supply is probably bad. Contact us for a
replacement.
If liquid
is not dispensed immediately after the pump is turned on,
the tubing was probably empty and needed to be
primed. If this is the case, the problem should not occur
as long as the tubing does not fill up again with air.
If liquid
is not dispensed or aspirated, the caps are probably
tightly screwed onto the bottles and air is not able to
enter or leave the bottles and equalize the pressure.
Loosen the caps on the bottles. If not caught early, this
could lead to tubing popping off a fitting or leakage at a
fitting.
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