Does manual processing of western blots produce inconsistent signals between repeats? BlotBot is the answer!
![BlotBot produces consistent signal strength EVERY TIME with consistent wash volumes and wash times](https://www.nextadvance.com/wp-content/uploads/BlotBotFeatures2.png)
Features
- Unattended dispensing and pouring off of liquids.
- Only requires an electrical outlet – no vacuum, pressurized air or gas, or additional pumps required.
- Can recover reagents, such as antibody solutions, separately from each gel or blot.
- Trays can hold mini blots (maximum dimension 8cm x 8cm) and midi blots (maximum dimension 8cm x 16cm) and strip blots (maximum dimension 4cm x 8cm; Blotbot 248 and 248V).
- Can switch between 2 wash buffers (BlotBot 248V only).
- Adjustable Parameters for each protocol step include:
- rocking speed (5 to 40 cycles per minute)
- reagent/solution rocking time (1 min to 96 hours)
- dispense volume (10 to 150 ml) up to 10 repetitions per step
- clearing rinse option
- Patented technology.
- Rocking continues after the final rinse and the wash buffer remains in the trays to prevent the blots or gels
from desiccating. - Built in the USA for years of trouble free use.
- Uses existing protocols. Programming is easy and follows standard protocols steps. Programs can be up to 9 steps
where each step is an immersion in a reagent (e.g. antibody) or a series of washes. - In each step reagents or buffer will be dispensed and aspirated to all the trays simultaneously, for consistent
processing of all the blots and gels. - The minimum volume required to fully cover each mini blot is 3.5 mL.
- A clearing rinse can be activated to provide a quick rinse to get the final drop of reagent after each wash or
reagent immersion. - Quiet.
- The removable trays can be washed. Replacements can be ordered separately.
- A bottle of buffer is connected to the Freedom Rocker BlotBot with a hose and leak-proof quick-connector connections.
- 30-day money-back guarantee.
- Two year warranty.
3 Key Advantages
![A small ammount of antibody passes over the blot as the BlotBot rocks](https://www.nextadvance.com/wp-content/uploads/Tray_sketch.jpg)
1. Use very small volumes of antibody solution.
Similar to blots in a rolling bottle, a small puddle of antibody passes repeatedly over the blot in curved bottom trays.
![recovered waste](https://www.nextadvance.com/wp-content/uploads/WasteRecovery_small.jpg)
2. Recover reagents separately.
Reagents from each blot are captured in 50 mL tubes, ready for storage.
![programing screen on the BlotBot](https://www.nextadvance.com/wp-content/uploads/TheBlotBotScreen_small.jpg)
3. Use your existing protocols.
Each program step matches each step in your protocol. For example, a 3X 10 min. buffer wash is a 3X 10 min. buffer wash. No development effort required.
![](https://www.nextadvance.com/wp-content/uploads/Features-Blot-Bot.jpg)
![Automated Processor for Western Blots](https://www.nextadvance.com/wp-content/uploads/TimersCartoon.jpg)
BlotBot Models
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$11,825.00 – $12,050.00 Select options This product has multiple variants. The options may be chosen on the product page
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$11,325.00 – $11,550.00 Select options This product has multiple variants. The options may be chosen on the product page
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$9,125.00 – $9,350.00 Select options This product has multiple variants. The options may be chosen on the product page