The easiest and coolest way to lyse cells and homogenize tissue.
"It's a very useful device for making tissue lysates. We no longer use sonication for DNA, RNA and protein preparation from multiple tissue samples."
Prof. Michael Sherman, Boston University School of Medicine
"The Bullet Blender has made our clinical lab much more efficient; we get consistent results in less time. We cannot imagine going back to hand vortexing for our whole blood extractions."
Kimberly Dumont, supervisor, Dartmouth Reference Lab
"I can count on it for fast consistent results."
Dr. Denis Schewe, Mt. Sinai Medical Center, New York City
"What I've found is that the instrument works very well, is faster than the Bead Beater, accommodates more samples and is considerably quieter... [I] get consistently uniform cell breakage."
Dr. Joseph Geisberg, Harvard Medical School
"This past January  we purchased a Bullet Blender from Next Advance. ... Let me begin by saying that we are extremely satisfied with the product! We have been able to implement it into a number of protocols that we use in our lab. Other labs in our department have also taken a liking to the unit and have been using it along side our lab."
Dr. Kyle Bender, Queen's University, Kingston, Ontario, CANADA
"The Bullet Blender® lets us reduce variability and user error and increase productivity."
Dr. Scott Gridley, Director of Protein Sciences, Blue Sky Biotech, Inc., Worcester, MA
"Using the Dounce homogenizer, I can process eight tumors in an hour and a half. With the Bullet Blender, I can homogenize twenty in fifteen minutes."
Dr. Alejandro Adam, Albany Medical Center, New York
"Our extraction staff have loved the Bullet Blenders, they have really helped with the workflow!" ... "They do a great job homogenizing for us, and save our techs from repetititve motion injuries, and help with time management. We love them!"
Chantry Clark, CDAT Lead Technologist, ARUP Labs, Utah
"I love it!! ... I'm going to try and get another one."
Shari Kohut, MLT ASCP, Women and Children's Hospital of Buffalo
"We used to use a mortar and pestle grinder and it took hours and hours and hours to homogenize our samples. The Bullet Blender has increased our throughput tremendously."
Dr. Christopher Ward, University of Maryland
"The Bullet Blender homogenizes our samples so well, our protein yield is almost too good."
Dr. Donna Newman, North Carolina State University
"It does work great for westerns ... It's a lot faster."
Dr. Timothy Bartness, Georgia State University
"That machine is a lifesaver! ... It worked out so well, we're contemplating purchasing a second one."
Dr. Lou Casta, Fraunhofer USA
"...the results are great! ... We have run it on the Agilent chip and the RNA integrity numbers (RIN) are 10 and 8.6 for both of the samples."
Phuong Nguyen, Translational Genomics Group, Baylor Health
"We use the Bullet Blender to disrupt mycobacteria and different types of gram-negative bacteria for a variety of applications and are very happy with the results we get."
Martin Pavelka, Ph.D., University of Rochester, School of Medicine and Dentistry
"I love the Bullet Blender! We work on the mouse model for chlaymdia and often have to remove the uteri from the mice and homogenize by hand. I used it the other day and was amazed how well it homogenized the tissue. We can do 24 samples in just 5 minutes-amazing. What used to take us hours we can do in just 5 minutes."
Cheryl Champion, Ph.D., Pathology & Laboratory Medicine, UCLA
The BULLET BLENDER® enables you to homogenize, disrupt, or lyse up to 24 tissue or cell culture samples at a time. Load the samples into standard polypropylene tubes, then place them in the BULLET BLENDER. The "bullets" in the instrument vigorously strike all of the tubes and homogenize your sample in minutes. NEW! The Bullet Blender is now available with Dry Ice Cooling™ to keep your samples at 4°C!
Liver, Spleen, Kidney, Brain, Adipose, Bladder, Eye, Thyroid, Gallbladder, Muscle, Pancreas, Testes, Meconium, Thymus, Adrenal gland, Lymph Node
Homogenize Tougher Tissue
Heart, Aorta, Tumor, Tail Snips, Colon, Lung, Blood Vessel, Trachea, Cartilage, Epithelium, Stomach, Intestine, Larynx, Mouse Femur, Pharynx, Umbilical Cord, Skin, Uterus, Ear, Polyp
Bacteria, Mammalian cell culture, Yeast
Homogenize Plant Material and Fungi
Leaves, Nuts, Roots, Mycelia, Moss, Tuber
Homogenize Small Organisms
Zebrafish, Insects, Nematode, Plankton
Aggresome extraction, Activity assays, Chromatin IP, Cytokine Assay, ELISA, HPLC, Immunoassay, Immunoblotting, Immunoprepitation, SDS-PAGE
Cloning, PCR, Library generation, Microarray, RT-PCR, qPCR, Sequencing, Forensics applications
Whole Cell Isolation
Bacteria, Lymphocytes, Blood Cells, Stem Cells, Primary Cell Isolation
HPLC, UPLC, GC-MS, LC-MS, Lipid Extraction, Drug Detection, Forensic Analysis
Liposome generation for drug delivery
See some publications referencing the Bullet Blender for these applications.
The Bullet Blender® Family
| NEW! BB24-AU Bullet Blender® GOLD
Colder, quieter, and faster homogenization!
- Dry Ice Cooling keeps samples at 4°C*
- Noise Insulation keeps homogenization quiet
- Available for 24 samples in snap cap microcentrifuge tubes or 8 samples in 50 mL tubes
- Perfect for tough samples such as mouse femur, tumor and skin
- Faster homogenization
BB24-AU Bullet Blender Gold...................................... more info
BB50-AU Bullet Blender Gold 50................................. more info
| BBY24M Bullet Blender® STORM
More powerful for tough samples!
- Perfect for tough samples such as mouse femur, tumor and skin
- Faster homogenization
- Up to 24 samples in snap cap microcentrifuge tubes
- Air cooling
- Available with CE certification
BBY24M Bullet Blender Storm...................................... more info
BBY24M-CE with CE certification................................. more info
|BBX24 Bullet Blender®
Our original Bullet Blender can homogenize up to 24 samples in microcentrifuge tubes. Ideal for samples up to 300mg. Also available with air cooling (blue models) and CE certification.
BBX24 Bullet Blender........................ more info
BBX24-CE with CE certification......... more info
BBX24B Bullet Blender Blue............. more info
BBX24B-CE with CE certification......
. more info
|BBX24W Bullet Blender® for
The Screw-Cap Bullet Blender can homogenize up to 24 samples in screw-cap microcentrifuge tubes. Ideal for samples up to 300mg. Also available with air cooling (blue models) and CE certification.
BBX24W Bullet Blender for
Screw Cap Tubes................................. more info
BBX24W-CE with CE certification......... more info
BBX24BW Bullet Blender Blue for
Screw Cap Tubes.................................. more info
BBX24BW-CE with CE certification....... more info
|BBY5M Bullet Blender® Blue 5
The Bullet Blender 5 can homogenize up to 12 samples in 5ml screw-cap tubes. Ideal for samples up to 1g. Air cooling included. CE certification available.
BBY5M Bullet Blender Blue 5......... more info
BBY5M-CE with CE certification..... more info
|BB50-DX Bullet Blender® 50 DX
The Bullet Blender 50 DX can homogenize up to 4 tough tissue samples or up to 8 soft samples in 50 mL tubes in parallel. Each tissue sample up to 3.5 g or cell culture combined with beads up to 20 ml.
Air cooling included.
BB50-DX Bullet Blender 50 DX......... more info
BB50-DX-CE with CE certification..... more info
Consistent and High Yield Results
The BULLET BLENDER prepares up to 24 samples in just minutes. All of the samples are automatically homogenized in the same manner, with high yields, so there is very little variation from sample to sample. See the Protocols pages for preparation times and speeds.
No Cross-Contamination or Sample Loss
There are no probes or blades to cause cross contamination or sample loss, which also means there are no parts to clean between runs. The sample tubes are kept closed and self-contained during homogenization.
Samples Stay COOL
No cool down required. The instrument uses very little power due to the unique, patented design, so there is very little heat build up in the instrument or inside the sample tubes. It uses a small DC motor to agitate the individual tubes. Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.
Easiest To Use
Simply place your sample, beads and buffer in standard tubes, and load the tubes into the BULLET BLENDER. Tune the extent of disruption by adjusting the run time and speed. Afterwards, place the sample tubes in a centrifuge to separate the lysate. No special holders are needed. There are no clamp plates nor locking rings to fiddle with.
Risk Free Purchase
BULLET BLENDER units come with a 30 day money back guarantee and a 2 year warranty. The simple, reliable design enables the BULLET BLENDER to sell for one half to one fifth the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker, cooler technique.
4 year extended warranty available.
Dry Ice and Air Cooling™ in The Bullet Blender®:
Gold models of the Bullet Blenders contain a dry ice cooling system. Fill the side compartment with dry ice- when the instrument is running, cold air will circulate through the inside of the instrument and the 4°C signal will light up when the temperature is reached. The air circulation will adjust according to homogenization conditions, allowing the contents of the tube to reach 4°C.
Blue and Storm model Bullet Blenders are equipped with Air Cooling™, which draws air into the Bullet Blender, keeping your samples at ambient temperature. This feature is particularly effective if your samples are sensitive to heat and you are using your Bullet Blender in a refrigerator or a cold room. The Air Cooling™ is also useful for preventing the inside of your Bullet Blender unit from heating up if you are running several consecutive homogenization cycles. Air Cooling™ is an option on the microtube models and standard on the 5 mL and the 50 mL tube models.
Air Cooling | Use and Operation
Want to know more about homogenization methods in general? Click here for an overview.
What is Air Cooling™?
The Air Cooling™ models have devices which exchange air with their surroundings; they do not have refrigeration units. Air Cooling™ effectively draws cold air into the instrument if you are using the Bullet Blender® in a cold room or a refrigerated cabinet. Air Cooling™ is also useful if you're running several sample cycles in a row or if your samples are extra sensitive to heating up. Normally, the samples in the Bullet Blender® only heat up a few degrees and stay quite cool. However, if you're running a second batch of samples soon afterward, they will heat up a little more because the Bullet Blender® is starting out slightly warmer. The Air Cooling™ keeps the Bullet Blender® cooler so that the first batch of samples heat up a little less and subsequent batches are kept just about as cool as the first batch.
Which models have Air Cooling™?
The Air Cooling™ feature comes on the "Blue" models: the Bullet Blender® Blue which uses microcentrifuge tubes, the Bullet Blender® Blue 5 which uses 5 mL tubes and the Bullet Blender® Blue 50 which uses 50 mL tubes. Air Cooling™ is also included with the Bullet Blender® Storm.
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Use and Operation:
Before using, please see our General Guidelines for Use
How is the Bullet Blender different from other high-throughput homogenizers on the market?
The Bullet Blender is similar to other Bead Mill Style homogenizers in that it processes each sample in its own tube using homogenization beads. Therefore, there is no chance of cross-contamination and there are no parts that need cleaning after each sample. A unique feature of the Bullet Blender is the way it agitates the sample tubes. Other homogenizers vigorously shake the sample tubes. This requires a great deal of energy and these instruments tend to be very large (because of the motor), expensive (many are 2 -3 times the cost of the Bullet Blender) and require cooling cycles due to motor heating - this can take a lot of time away from experiments. The Bullet Blender, on the other hand, uses a patented striking method to agitate tubes. This requires a much smaller motor and generates much less heat. Therefore the Bullet Blender is smaller, lighter, and less expensive than competitor's equipment while working just as well. The Bullet Blender can also be used in a cold room unlike many of the other instruments on the market.
What types of tubes can be used?
The Bullet Blender® Storm works with 1.5ml Eppendorf® Safe-Lock tubes. The Bullet Blender® and Bullet Blender® Blue work with 1.5 mL, 1.7 mL, and 2.0 mL polypropylene sample tubes, including conical and round bottom microcentrifuge snap-cap tubes. We recommend Eppendorf® Safe-Lock tubes. When using 2ml tubes in the the Bullet Blender® or the Bullet Blender® Blue we recommend using a minimum of 2 tubes per homogenization cycle. The screw cap models use Axygen® and Corning® 1.5 ml screw cap tubes. The Bullet Blender® Blue 5 units work with 5ml Axygen® brand screw top transport tubes. The Bullet Blender 50-DX units work with 50 mL skirted (self-standing) polypropylene tubes, such as Axygen® brand or Corning® brand. The slightly hazy polypropylene tubes are tougher and resist the striking better than the clearer tubes, which might crack at higher speeds. Use high quality tubes, rather than bargain brand tubes which are not as strong.
How do you set the Bullet Blender® to mix, disrupt, or homogenize different types of samples?
You can adjust the speed and the duration. Adjusting the speed affects how firmly the balls will strike the sample tubes, while adjusting the duration affects the total number of impacts. Homogenizing tough tissue requires longer durations at full speed, while mixing requires lower speeds.
How easy is it to operate the Bullet Blender®?
Simply place up to 24 sample tubes in the holders, set the desired duration and speed, and push start. All the samples will be processed simultaneously, without any chance of cross contamination.
How much volume of sample can be homogenized in each microcentrifuge tube?
For the BBX24 model Bullet Blenders, we recommend using 10-300mg of tissue in a standard microcentrifuge tube. Less than 10mg will work, but it requires careful attention to the amount of beads and volume of homogenization buffer. As with any experiment, errors become larger as the measured quantity gets smaller, so reproducibility becomes an issue. Samples larger than 300mg are not recommended. The air volume inside the tube is critical to allow the sample to be ground up inside the tube, so if the tube is more than 75% full, the efficiency of the homogenization is reduced. The 5 mL tubes can hold up to about 1 g of sample tissue or cultured cells, and a total of 3.5 mL of sample, buffer, and beads combined. The 50 mL tubes can hold up to about 3.5 g of sample tissue or cultured cells, and a total of 20 mL of sample, buffer, and beads combined.
Do sample tubes need to be placed symmetrically?
No. There are no restrictions as to which holes to place your sample tubes in, however you may get better results if you space them apart as evenly as possible.
How do you retrieve the sample from the tubes?
After the Bullet Blender® stops, centrifuge the tubes to clarify the lysate prior to use in your molecular biology applications.
Which beads should I use?
The protocols page contains suggested beads, speeds, and durations for processing many types of tissues and cells. The Bullet Blender®beads page has a bead selection table. The two basic rules for bead selection: (1) use denser beads for tougher samples, and (2) use beads of a comparable size to the size of your samples to maximize the effectiveness of collisions between beads and samples. For example, if you wanted to lyse bacterial cells with a soft membrane, use small glass beads. If you want to homogenize larger pieces of tough tissue, use large steel beads. You can also contact our technical support staff at firstname.lastname@example.org if you have any questions.
I am trying to process tough samples in the Bullet Blender® and the homogenization is incomplete. How can I improve my results?
There are several ways that you can increase the homogenization of your samples.
- Examine the shape of your samples- long thin samples will homogenize faster than cube-shaped or round samples.
- Try cutting your samples into smaller pieces.
- If you are running many samples at once in the Bullet Blender, try runing fewer tubes at a time. If a sample is extremely tough (e.g. mouse femur) you may only be able to achieve complete homogenization with 2-4 tubes at a time.
- If you are using the BB50-DX make sure that all of the sleeves are removed except for the sleeves containing tubes.
I previously owned another bead mill homogenizer. Can I use the same protocols that we developed for the other homogenizer with the Bullet Blender®?
No, the mechanism of action is different from all other homogenizers on the market. Our patented striking technology makes the Bullet Blender both highly efficient and effective. If you use another product's protocol with the Bullet Blender, you are very likely to experience different results. Please use one of our established protocols, or empirically determine a protocol that works best for your samples.
We would like to use Fisherbrand tubes with the Bullet Blender®. Do they perform well?
Due to difference in shape, Fisherbrand tubes are more susceptible to wear in the Bullet Blender. We therefore recommend that you avoid using Fisherbrand tubes with the Bullet Blender. If you must use Fisherbrand tubes, do not use them in runs of more than four minutes.
Why is there a three tube minimum for Bullet Blender® 5 and a two tube minimum for the Bullet Blender® 50-DX?
These Bullet Blender models contain larger motors. If all the energy were directed at a single tube, the tube might break. Tubes partly filled with water can be used in place of a tube filled with sample.
Is it possible to homogenize tissue that has been frozen in liquid nitrogen?
It is possible to homogenize tissue that was frozen in liquid nitrogen. Immerse the frozen tissue in cold buffer and allow it to thaw, then treat it as you would any other sample. If the tissue was dried before it was frozen, you can pulverize the tissue by running the tissue with beads only (no buffer), then adding the cold buffer and running again to complete the homogenization. You cannot homogenize "wet" tissue while it is still frozen, as it will effectively be a block of ice and homogenization efficiency will be very poor.
What is the "Key-Switch Lock Out" option for Bullet Blenders?
This key switch option incapacitates the electronics, preventing others from operating your Bullet Blender. Thereby, others are discouraged from working in your area, using your supplies, and upsetting your experiments.
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Contact us if you have any more questions.