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The easiest and
coolest
way
to lyse cells and homogenize tissue
No cross contamination
worries!
And samples stay cool! |
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The Bullet Blender™
Homogenize
24 samples at a time!
The BULLET BLENDER™ enables
you to homogenize, disrupt, or lyse 24 tissue or cell
culture
samples at a time. Load the samples in
standard polypropylene microcentrifuge tubes into
the
BULLET BLENDER™.
The "bullets" in the "blender" vigorously strike all
the sample tubes simultaneously for a few
minutes.
Now available with Air
Cooling™ to keep samples even
cooler!
>
Fast,
Consistent Results
>
No Cross
Contamination
>
Samples Stay
Cool
>
Convenient
>
Risk Free
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Applications:
homogenizing cell cultures such as yeast and
E. coli to tough tissues such as cartilage,
tumors,
heart muscle and hair, as well as organs such as liver, kidney, brain,
lung, and skin, and samples such as mouse/rat
tails, embryos, C. elegans,
plant matter, and more.
It's also very useful for long duration vortexing in
applications such as phenol chloroform extraction
and
blood processsing. |
|
DNA |
RNA |
Protein |
|
Genomic DNA extraction from
plant samples |
RT-PCR results from homogenized
carcinoma
samples |
Protein samples on Coomassie stained SDS-PAGE
gel from
E. coli
|
Lanes 1-4 Tobacco leaf DNA
samples
λ Hind III
Lanes 5-8 Rice leaf DNA samples |
 Lanes 1-7: RT-PCR
products from tumor RNA samples |
wc = whole cell
sf = soluble fraction
Left two lanes control
Right two lanes induced |
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“It's a very useful device for
making tissue lysates. We no longer use sonication
for DNA, RNA and protein preparation from multiple tissue
samples.”
Prof. Michael Sherman, Boston
University School of Medicine
“The
Bullet Blender has made our clinical lab much more
efficient; we get consistent results in less time.
We cannot imagine going back to hand vortexing for our
whole blood extractions.”
Kimberly Dumont, supervisor, Dartmouth Reference Lab
“I can count on it for fast
consistent results.”
Dr. Denis
Schewe, Mt. Sinai Medical Center, New York City
“The Bullet Blender™ lets us reduce variability and user
error and increase productivity.”
Dr.
Scott Gridley, Director of Protein Sciences, Blue Sky
Biotech, Inc., Worcester, MA. |
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The Bullet Blender™
Family
The BULLET BLENDER™
enables you to disrupt or homogenize many samples at
a time, by incorporating
patented striking technology.
The sample tubes are forcefully agitated thousands
of times per minute, inducing vigorous motion of the
substances inside the tubes.
Simply place your sample, some
buffer, and optionally some beads in standard
polypropylene tubes. Place the tubes in the BULLET BLENDER™
and run for a few minutes. Afterwards,
place the sample tubes in a centrifuge to separate
the lysate.
Because the samples
are in separate tubes and no probes contact the
samples, cross contamination is very easy to
prevent.
The patented design requires very
little power, so the motor will last for years.
Also, the motor will not heat up significantly, so
the samples stay cool.
The addition of beads in the sample tubes allows disruption or thorough
homogenization of cells and tissues. Tune the
extent of disruption by adjusting the speed.
Air Cooling™
is an option on the microtube model and standard on
the 50 mL tube model.
The Air Cooling™
Air Cooling™
entails fans which exchange air with their
surroundings; they have nothing that actually chills
the instrument. Air Cooling™
is useful if you're running several sample cycles in
a row or if your samples are extra sensitive to
heating up. Normally, the samples in the Bullet
Blender™
only heat up a few degrees and stay quite cool.
However, if you're running a second batch of samples
soon afterwards, they will heat up a little more
because the Bullet Blender™
is starting out slightly warmer. The Air Cooling™
keeps the Bullet Blender™
cooler so that the first batch of samples heat up a
little less and subsequent batches are kept just
about as cool as the first batch.
Click here to see
sample protocols or answers to
frequently asked questions (FAQs).
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The Bullet Blender™
BLUE
50
Similar to the original Bullet Blender™, this model holds up
to 9 of 50 mL tubes at a time.
The sample, lysis buffer, and beads should occupy no
more than 15 ml of any tube to allow plenty of room
for the sample and beads to slosh around.
While this version is not as good for tough tissue,
it is ideal for cell culture and larger volume soft
tissue samples, such as entire rat lungs. This
model requires
BD Falcon™
or Krackeler brand polypropylene 50 ml tubes; tubes
that are a slightly different size may not work
properly. Because of the longer processing times
(about 3X longer than with microcentrifuge tubes),
this version is only available with
Air Cooling™.
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The 3 BULLET BLENDER™
models
| |
Bullet Blender™ |
Bullet Blender™
BLUE |
Bullet Blender™ BLUE
50 |
|
Capacity |
24
Tubes |
24
Tubes |
9
Tubes |
|
Tubes |
1.5 to 2.0 mL polypropylene microtubes
(e.g. Axygen brand) |
50
mL polypropylene
(e.g. Falcon™) |
|
Maximum Fill Volume |
1.0 to 1.5 mL |
15 mL |
|
Air Cooling™? |
No |
Yes |
Yes |
|
Applications |
Vortexing, Homogenizing Cell Cultures and Soft to Very Tough
Tissue |
Vortexing, Homogenizing Cell Cultures and Soft Tissue |
>
Fast, Consistent Results
The BULLET BLENDER™
prepares up to 24 samples in just minutes. All
of the samples are automatically homogenized the
same, so there is very little variation from sample
to sample. See the
Protocols pages for preparation times and speeds.
>
No Cross Contamination or Sample Loss
There are no probes or blades to cause cross
contamination or sample loss. The sample
tubes are kept closed during agitation, as the cells
or tissue are disrupted. There are no probes to
clean between samples.
>
Samples Stay
Cool
The instrument uses very
little power due to the unique, patented design, so
there is very little heat build up in the instrument
or inside the sample tubes.
It uses a small DC motor to agitate the individual
tubes, not ultrasonics. Also, because
the motor does not need to agitate any heavy
platforms or plates, it is small and will last for
years.
>
Convenient to Use
Simply place your cells or
tissue, and optionally some beads, in
standard
tubes, and load the tubes into the BULLET BLENDER™.
Set the duration (typically
several
minutes) and speed (vigorousness). There are no probes to clean.
It is much quieter than a sonicator and the
samples do not heat up more than a few degrees!
>
Risk Free Purchase
The BULLET BLENDERS™
come with a 30 day money back guarantee and a 2 year
warranty. The simple, reliable design enables the BULLET BLENDERS™ to
sell for one half to one fifth the price of ultrasonic or
other agitation based instruments, yet provides an easier,
quicker cooler technique. |
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“This
past January we purchased a Bullet Blender from Next
Advance. ... Let me begin by saying that we are
extremely satisfied with the product! We have been able to
implement it into a number of protocols that we use in our
lab. Other labs in our department have also taken a
liking to the unit and have been using it along side our
lab.”
Dr. Kyle Bender,
Queen's University, Kingston, Ontario, CANADA
“Using the Dounce homogenizer, I can process eight tumors
in an hour and a half. With the Bullet Blender™, I
can homogenize twenty in fifteen minutes.”
Dr. Alejandro Adam, University at
Albany (SUNY), New York |
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FEATURES
-
Compact. Only 8 in. (20 cm) wide by 10 in. (25 cm)
deep by 7.5 in. (19 cm) high.
-
Capacity.
Bullet Blender™
and
Bullet Blender™
BLUE:24 of 1.5 mL
to 2.0 mL polypropylene
sample (microcentrifuge) tubes. Bullet Blender™
BLUE 50: 9
of 50 mL polypropylene tubes.
-
Can use in
cold rooms and incubators, 4°C to 60°C.
(Avoid condensation.)
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Can use most anywhere.
Only requires an electrical outlet. -
CE/UL certified 24 volt power supply for
wall outlets worldwide.
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Two adjustable Parameters:
- duration
(Bullet Blender™
and
Bullet Blender™
BLUE:
1 to 5 min.;
Bullet Blender™ BLUE 50:
3 to 15 min.) and momentary on (for quick mixing).
- speed
(slow for vortexing to fast for vigorous agitation).
-
User friendly, one-touch
operation.
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Samples stay
cool.
The samples do not heat up more than a few degrees
C.
-
Air Cooling on "BLUE"
models, which keeps the instrument a little cooler and
prevents the instrument from heating up more when
processing multiple rounds of samples. The
COOLEST homogenizer
available!
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Patented technology.
-
Built in the USA for
years of trouble free use.
-
Warranty: 2 years parts and labor.
3 years on motor.
-
30 day money back guarantee.
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BULLET BLENDER™ Brochure
BULLET BLENDER™ FAQs
and
Protocols
This graph illustrates a sample
application: homogenizing up to 24 samples of chicken
muscle in just 3 minutes. Each tube had
0.2 g chicken muscle in polysome lysis buffer (0.5% NP-40, 100mM KCl,
10mM HEPES, 5mM MgCl2) with 0.2 g of
0.5 mm diameter glass beads. The samples were
centrifuged to collect the
protein. Note that more protein was recovered
using 0.5 mL buffer (in 2.0 mL conical bottom tubes) than
with 1.0 mL buffer (in 1.5 mL tubes); other volumes
of buffer did not work as well. The amount of
protein was determined using a Bradford assay. |
Photo
of the Bullet Blender™ with the cover open showing 3
sample tubes in place. |
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CDX24 BULLET
BLENDER™ ........... $1,895
(plus shipping and taxes)
For
use with up to 24 microcentrifuge tubes at a time.
Includes the following:
·
Bullet Blender™ instrument.
·
operator's manual.
·
24V DC Wall plug power supply (UL/CE).
·
seven
10 mL tubes
each filled with beads in a different material or size, from 0.1mm to 2.0 mm.
· spatula
for dispensing beads.
CDX24B BULLET
BLENDER™BLUE ........... $2,195
(plus shipping and taxes)
Similar to the
original Bullet Blender™ with Air
Cooling™, which keeps the samples even cooler and prevents the instrument and
samples from additional heating up when processing
multiple rounds of samples. The 3rd, 4th, or 10th round of samples barely
heat up more than the first round.
CDX50B BULLET
BLENDER™ BLUE 50 ........... $2,495
(plus shipping and taxes)
For use with 50 mL tubes, up to 9 at a time, and has Air
Cooling™.
Includes the following:
·
Bullet Blender™ 50
BLUE instrument.
·
operator's manual.
·
24V DC Wall plug power supply (UL/CE).
·
seven
10 mL tubes, each
filled with beads in a different material or size, from 0.1mm to 4.8 mm.
· spatula
for dispensing beads.
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TUBES
TUBE1AX7 .......... $25
(plus shipping and taxes)
Box of 500 of 1.7 mL non-sterile Axygen brand polypropylene microtubes, in bulk.
For use in the BULLET BLENDER™ (cat. no. CDX24) and BULLET BLENDER™BLUE
(cat. no. CDX24B)
TUBE1AX7-S .......... $20
(plus shipping and taxes)
Box of 250 of 1.7 mL sterile Axygen brand polypropylene microtubes, in bulk.
For use in the BULLET BLENDER™ (cat. no. CDX24) and BULLET BLENDER™BLUE
(cat. no. CDX24B)
TUBE50KR-S .......... $15
(plus shipping and taxes)
Bag of 50 in bulk, of 50 mL sterile polypropylene centrifuge tubes.
For use in the BULLET BLENDER™BLUE 50
(cat. no. CDX50B)
SPATULAS
SPAT1 .......... $180
(plus shipping and taxes)
Individually
packaged spatulas, RNase/DNase free, non-pyrogenic and antistatic.sterile,
plastic spatulas. pack of 50.
BEADS
You can also order beads on the
beads webpage, which contains FAQs regarding beads, as well as additional
selection information, including a
bead selection table.
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Glass beads
(2.5 g/cc)
$30 per lb. (.45 kg)
 |
Zirconium Silicate
beads (3.8 g/cc)
$40 per lb. (.45 kg)
|
Zirconium Oxide
beads (5.5 g/cc)
$75 per lb. (.45 kg)
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Stainless Steel
Beads (7.9 g/cc)
$60 - 145 per lb.
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Bead Sample Packs include 10 mL tubes
of various beads. (1 tube of each type of bead) |
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Bead Selection
Guide
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Glass |
Zirconium Silicate (ZrSiO) |
Zirconium
Oxide (ZrO) |
Stainless
Steel |
|
Density (g/cc) |
2.5 |
3.8 |
5.5 |
7.9 |
|
Bead Diameter (mm) |
0.1 |
0.5 |
1.0 |
0.5 |
1.0 |
2.0 |
0.15 |
0.5 |
1.0 |
2.0 |
0.2 |
0.5 |
0.9 to 2.0 Blend |
3.2 |
4.8 |
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S. cerevisiae / fungi |
√ |
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E. coli / bacteria |
√ |
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C. elegans / briggsae |
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√ |
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mammalian cell culture |
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Drosophila / insects |
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plant tissue |
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