Heart Tissue Homogenizer & Homogenization Protocol

Ideal for Heart Tissue Homogenization

Do you spend lots of time and effort homogenizing heart tissue samples? The Bullet Blender® tissue homogenizer delivers high quality and superior yields. No other homogenizer comes close to delivering the Bullet Blender’s winning combination of top-quality performance and budget-friendly affordability. See below for a heart tissue homogenization protocol.

Save Time, Effort and Get Superior Results with

The Bullet Blender Homogenizer

Consistent and High Yield Results

Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g.

No Cross Contamination

No part of the Bullet Blender ever touches the tissue – the sample tubes are kept closed during homogenization. There are no probes to clean between samples.

Samples Stay Cool

The Bullet Blenders’ innovative and elegant design provides convective cooling of the samples, so they do not heat up more than several degrees. In fact, our Gold+ models hold the sample temperature to about 4ºC.

Easy and Convenient to Use

Just place beads and buffer along with your tissue sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start.

Risk Free Purchase

Thousands of peer-reviewed journal articles attest to the consistency and quality of the Bullet Blender homogenizer. We offer a 2 year warranty, extendable to 4 years, because our Bullet Blenders are reliable and last for many years.  

Heart Tissue Homogenization Protocol

Sample size

See the Protocol
microcentrifuge tube model (up to 300 mg) Small heart samples
5mL tube model (100mg - 1g) Medium heart samples
50mL tube model (100mg - 3.5g) Large heart samples

What Else Can You Homogenize? Tough or Soft, No Problem! 

The Bullet Blender can process a wide range of samples including organ tissue, cell culture, plant tissue, and small organisms. You can homogenize samples as tough as mouse femur or for gentle applications such as tissue dissociation or organelle isolation.

the Bullet Blender high-throughput tissue homogenizer

Heart tissue pieces (on beads in upper photo) are completely homogenized into the buffer (darker in lower photo). 

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    Bullet Blender Models

    Select Publications using the Bullet Blender to Homogenize Heart Tissue

    See all of our Bullet Blender publications!

    Malania, L., Bai, Y., Osikowicz, L. M., Tsertsvadze, N., Katsitadze, G., Imnadze, P., & Kosoy, M. (2016). Prevalence and Diversity of Bartonella Species in Rodents from Georgia (Caucasus). The American Journal of Tropical Medicine and Hygiene, 16–0041. https://doi.org/10.4269/ajtmh.16-0041
    Zhang, F., Hartnett, S., Sample, A., Schnack, S., & Li, Y. (2016). High fat diet induced alterations of atrial electrical activities in mice. American Journal of Cardiovascular Disease, 6(1), 1–9. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788723/
    Hernandez-Anzaldo, S., Berry, E., Brglez, V., Leung, D., Yun, T. J., Lee, J. S., Filep, J. G., Kassiri, Z., Cheong, C., Lambeau, G., Lehner, R., & Fernandez-Patron, C. (2015). Identification of a Novel Heart-Liver Axis: Matrix Metalloproteinase-2 Negatively Regulates Cardiac Secreted Phospholipase A2 to Modulate Lipid Metabolism and Inflammation in the Liver. Journal of the American Heart Association, 4(11), e002553–e002553. https://doi.org/10.1161/JAHA.115.002553
    Falendysz, E. A., Lopera, J. G., Lorenzsonn, F., Salzer, J. S., Hutson, C. L., Doty, J., Gallardo-Romero, N., Carroll, D. S., Osorio, J. E., & Rocke, T. E. (2015). Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PLOS Neglected Tropical Diseases, 9(10), e0004130. https://doi.org/10.1371/journal.pntd.0004130
    Pappas, C. T., Mayfield, R. M., Henderson, C., Jamilpour, N., Cover, C., Hernandez, Z., Hutchinson, K. R., Chu, M., Nam, K.-H., Valdez, J. M., Wong, P. K., Granzier, H. L., & Gregorio, C. C. (2015). Knockout of Lmod2 results in shorter thin filaments followed by dilated cardiomyopathy and juvenile lethality. Proceedings of the National Academy of Sciences, 201508273. https://doi.org/10.1073/pnas.1508273112
    Sjögren, B., Swaney, S., & Neubig, R. R. (2015). FBXO44-Mediated Degradation of RGS2 Protein Uniquely Depends on a Cullin 4B/DDB1 Complex. PLOS ONE, 10(5), e0123581. https://doi.org/10.1371/journal.pone.0123581
    Berry, E., Hernandez-Anzaldo, S., Ghomashchi, F., Lehner, R., Murakami, M., Gelb, M. H., Kassiri, Z., Wang, X., & Fernandez-Patron, C. (2015). Matrix Metalloproteinase-2 Negatively Regulates Cardiac Secreted Phospholipase A2 to Modulate Inflammation and Fever. Journal of the American Heart Association, 4(4), e001868–e001868. https://doi.org/10.1161/JAHA.115.001868
    Schisler, J. C., Grevengoed, T. J., Pascual, F., Cooper, D. E., Ellis, J. M., Paul, D. S., Willis, M. S., Patterson, C., Jia, W., & Coleman, R. A. (2015). Cardiac Energy Dependence on Glucose Increases Metabolites Related to Glutathione and Activates Metabolic Genes Controlled by Mechanistic Target of Rapamycin. Journal of the American Heart Association, 4(2), e001136–e001136. https://doi.org/10.1161/JAHA.114.001136
    Konhilas, J. P., Chen, H., Luczak, E., McKee, L. A., Regan, J., Watson, P. A., Stauffer, B. L., Khalpey, Z. I., Mckinsey, T. A., Horn, T., LaFleur, B., & Leinwand, L. A. (2015). Diet and sex modify exercise and cardiac adaptation in the mouse. AJP: Heart and Circulatory Physiology, 308(2), H135–H145. https://doi.org/10.1152/ajpheart.00532.2014
    Doldur-Balli, F., Ozel, M. N., Gulsuner, S., Tekinay, A. B., Ozcelik, T., Konu, O., & Adams, M. M. (2015). Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ). BMC Neuroscience, 16(1). https://doi.org/10.1186/s12868-015-0229-4
    Koren, L., Alishekevitz, D., Elhanani, O., Nevelsky, A., Hai, T., Kehat, I., Shaked, Y., & Aronheim, A. (2015). ATF3-dependent cross-talk between cardiomyocytes and macrophages promotes cardiac maladaptive remodeling. International Journal of Cardiology, 198, 232–240. https://doi.org/10.1016/j.ijcard.2015.06.099
    Manning, J. R., Withers, C. N., Levitan, B., Smith, J. D., Andres, D. A., & Satin, J. (2015). Loss of Rad-GTPase produces a novel adaptive cardiac phenotype resistant to systolic decline with aging. American Journal of Physiology - Heart and Circulatory Physiology, 309(8), H1336–H1345. https://doi.org/10.1152/ajpheart.00389.2015
    Kolb, T. M., Peabody, J., Baddoura, P., Fallica, J., Mock, J. R., Singer, B. D., D’Alessio, F. R., Damarla, M., Damico, R. L., & Hassoun, P. M. (2015). Right ventricular angiogenesis is an early adaptive response to chronic hypoxia-induced pulmonary hypertension. Microcirculation, n/a-n/a. https://doi.org/10.1111/micc.12247
    Hezel, M. P., Liu, M., Schiffer, T. A., Larsen, F. J., Checa, A., Wheelock, C. E., Carlström, M., Lundberg, J. O., & Weitzberg, E. (2015). Effects of long-term dietary nitrate supplementation in mice. Redox Biology, 5, 234–242. https://doi.org/10.1016/j.redox.2015.05.004
    Neishabouri, S. H., Hutson, S. M., & Davoodi, J. (2015). Chronic activation of mTOR complex 1 by branched chain amino acids and organ hypertrophy. Amino Acids, 47(6), 1167–1182. https://doi.org/10.1007/s00726-015-1944-y
    Mootz, J. M., Benson, M. A., Heim, C. E., Crosby, H. A., Kavanaugh, J. S., Dunman, P. M., Kielian, T., Torres, V. J., & Horswill, A. R. (2015). Rot is a key regulator of Staphylococcus aureus biofilm formation: Rot regulates S . aureus biofilm formation. Molecular Microbiology, 96(2), 388–404. https://doi.org/10.1111/mmi.12943
    Lemon, D. D., Harrison, B. C., Horn, T. R., Stratton, M. S., Ferguson, B. S., Wempe, M. F., & McKinsey, T. A. (2015). Promiscuous actions of small molecule inhibitors of the protein kinase D-class IIa HDAC axis in striated muscle. FEBS Letters, 589(10), 1080–1088. https://doi.org/10.1016/j.febslet.2015.03.017
    Eriksson, A., Williams, M. J., Voisin, S., Hansson, I., Krishnan, A., Philippot, G., Yamskova, O., Herisson, F. M., Dnyansagar, R., Moschonis, G., Manios, Y., Chrousos, G. P., Olszewski, P. K., Frediksson, R., & Schiöth, H. B. (2015). Implication of coronin 7 in body weight regulation in humans, mice and flies. BMC Neuroscience, 16(1), 13. https://doi.org/10.1186/s12868-015-0151-9
    Zhang, Z., He, L., Hu, S., Wang, Y., Lai, Q., Yang, P., Yu, Q., Zhang, S., Xiong, F., Simsekyilmaz, S., Ning, Q., Li, J., Zhang, D., Zhang, H., Xiang, X., Zhou, Z., Sun, H., & Wang, C.-Y. (2015). AAL exacerbates pro-inflammatory response in macrophages by regulating Mincle/Syk/Card9 signaling along with the Nlrp3 inflammasome assembly. American Journal of Translational Research, 7(10), 1812–1825.
    Song, B., Liu, Y., Parman, T., Liu, S., Miller, J. K., Liu, X., Tanga, M. J., & Mirsalis, J. (2014). Quantitative Proteomics for Cardiac Biomarker Discovery Using Isoproterenol-Treated Nonhuman Primates. Journal of Proteome Research, 13(12), 5909–5917. https://doi.org/10.1021/pr500835w
    Drake, J. C., Bruns, D. R., Peelor, F. F., Biela, L. M., Miller, R. A., Hamilton, K. L., & Miller, B. F. (2014). Long-lived crowded-litter mice have an age-dependent increase in protein synthesis to DNA synthesis ratio and mTORC1 substrate phosphorylation. AJP: Endocrinology and Metabolism, 307(9), E813–E821. https://doi.org/10.1152/ajpendo.00256.2014
    Ablorh, N.-A. D., Dong, X., James, Z. M., Xiong, Q., Zhang, J., Thomas, D. D., & Karim, C. B. (2014). Synthetic Phosphopeptides Enable Quantitation of the Content and Function of the Four Phosphorylation States of Phospholamban in Cardiac Muscle. Journal of Biological Chemistry, 289(42), 29397–29405. https://doi.org/10.1074/jbc.M114.556621
    van der Plas-Duivesteijn, S. J., Mohammed, Y., Dalebout, H., Meijer, A., Botermans, A., Hoogendijk, J. L., Henneman, A. A., Deelder, A. M., Spaink, H. P., & Palmblad, M. (2014). Identifying Proteins in Zebrafish Embryos Using Spectral Libraries Generated from Dissected Adult Organs and Tissues. Journal of Proteome Research, 13(3), 1537–1544. https://doi.org/10.1021/pr4010585
    Wang, X., Huang, W., Yang, Y., Wang, Y., Peng, T., Chang, J., Caldwell, C. C., Zingarelli, B., & Fan, G.-C. (2014). Loss of duplexmiR-223 (5p and 3p) aggravates myocardial depression and mortality in polymicrobial sepsis. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1842(5), 701–711. https://doi.org/10.1016/j.bbadis.2014.01.012
    Williams, S. M., Golden-Mason, L., Ferguson, B. S., Schuetze, K. B., Cavasin, M. A., Demos-Davies, K., Yeager, M. E., Stenmark, K. R., & McKinsey, T. A. (2014). Class I HDACs regulate angiotensin II-dependent cardiac fibrosis via fibroblasts and circulating fibrocytes. Journal of Molecular and Cellular Cardiology, 67, 112–125. https://doi.org/10.1016/j.yjmcc.2013.12.013
    Parra, S., Huang, X., Charbeneau, R. A., Wade, S. M., Kaur, K., Rorabaugh, B. R., & Neubig, R. R. (2014). Conditional disruption of interactions between Gαi2 and regulator of G protein signaling (RGS) proteins protects the heart from ischemic injury. BMC Pharmacology and Toxicology, 15(1), 29. https://doi.org/10.1186/2050-6511-15-29
    Cavasin, M. A., Demos-Davies, K. M., Schuetze, K. B., Blakeslee, W. W., Stratton, M. S., Tuder, R. M., & McKinsey, T. A. (2014). Reversal of severe angioproliferative pulmonary arterial hypertension and right ventricular hypertrophy by combined phosphodiesterase-5 and endothelin receptor inhibition. Journal of Translational Medicine, 12(1), 314. https://doi.org/10.1186/s12967-014-0314-y
    Melero, M., García-Párraga, D., Corpa, J., Ortega, J., Rubio-Guerri, C., Crespo, J., Rivera-Arroyo, B., & Sánchez-Vizcaíno, J. (2014). First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens). BMC Veterinary Research, 10(1), 968. https://doi.org/10.1186/s12917-014-0308-2
    Lassaletta, A. D., Elmadhun, N. Y., Burgess, T. A., Bianchi, C., Sabe, A. A., Robich, M. P., Chu, L. M., & Sellke, F. W. (2014). Microvascular Notch Signaling Is Upregulated in Response to Vascular Endothelial Growth Factor and Chronic Myocardial Ischemia. Circulation Journal, 78(3), 743–751. https://doi.org/10.1253/circj.CJ-13-0685
    Drake, J. C., Peelor, F. F., Biela, L. M., Watkins, M. K., Miller, R. A., Hamilton, K. L., & Miller, B. F. (2013). Assessment of Mitochondrial Biogenesis and mTORC1 Signaling During Chronic Rapamycin Feeding in Male and Female Mice. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 68(12), 1493–1501. https://doi.org/10.1093/gerona/glt047
    Cheng, X., Guo, S., Liu, Y., Chu, H., Hakimi, P., Berger, N. A., Hanson, R. W., & Kao, H.-Y. (2013). Ablation of Promyelocytic Leukemia Protein (PML) Re-patterns Energy Balance and Protects Mice from Obesity Induced by a Western Diet. Journal of Biological Chemistry, 288(41), 29746–29759. https://doi.org/10.1074/jbc.M113.487595
    Lassaletta, A. D., Elmadhun, N. Y., Liu, Y., Feng, J., Burgess, T. A., Karlson, N. W., Laham, R. J., & Sellke, F. W. (2013). Ethanol Promotes Arteriogenesis and Restores Perfusion to Chronically Ischemic Myocardium. Circulation, 128(11_suppl_1), S136–S143. https://doi.org/10.1161/CIRCULATIONAHA.112.000207
    Koren, L., Elhanani, O., Kehat, I., Hai, T., & Aronheim, A. (2013). Adult Cardiac Expression of the Activating Transcription Factor 3, ATF3, Promotes Ventricular Hypertrophy. PLoS ONE, 8(7), e68396. https://doi.org/10.1371/journal.pone.0068396
    Miller, B. F., Robinson, M. M., Reuland, D. J., Drake, J. C., Peelor, F. F., Bruss, M. D., Hellerstein, M. K., & Hamilton, K. L. (2013). Calorie Restriction Does Not Increase Short-term or Long-term Protein Synthesis. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 68(5), 530–538. https://doi.org/10.1093/gerona/gls219
    Lassaletta, A. D., Chu, L. M., Elmadhun, N. Y., Robich, M. P., Hoffman, Z. G., Kim, D. J., & Sellke, F. W. (2013). Mechanism for reduced pericardial adhesion formation in hypercholesterolemic swine supplemented with alcohol. European Journal of Cardio-Thoracic Surgery, 43(5), 1058–1064. https://doi.org/10.1093/ejcts/ezs488
    Wang, H., Sreenivasan, U., Gong, D.-W., O’Connell, K. A., Dabkowski, E. R., Hecker, P. A., Ionica, N., Konig, M., Mahurkar, A., Sun, Y., Stanley, W. C., & Sztalryd, C. (2013). Cardiomyocyte-specific perilipin 5 overexpression leads to myocardial steatosis and modest cardiac dysfunction. The Journal of Lipid Research, 54(4), 953–965. https://doi.org/10.1194/jlr.M032466
    Moghadaszadeh, B., Rider, B. E., Lawlor, M. W., Childers, M. K., Grange, R. W., Gupta, K., Boukedes, S. S., Owen, C. A., & Beggs, A. H. (2013). Selenoprotein N deficiency in mice is associated with abnormal lung development. The FASEB Journal, 27(4), 1585–1599. https://doi.org/10.1096/fj.12-212688
    Al-Hasan, Y. M., Evans, L. C., Pinkas, G. A., Dabkowski, E. R., Stanley, W. C., & Thompson, L. P. (2013). Chronic Hypoxia Impairs Cytochrome Oxidase Activity Via Oxidative Stress in Selected Fetal Guinea Pig Organs. Reproductive Sciences, 20(3), 299–307. https://doi.org/10.1177/1933719112453509
    Hoang, H. D., Prasain, J. K., Dorand, D., & Miller, M. A. (2013). A Heterogeneous Mixture of F-Series Prostaglandins Promotes Sperm Guidance in the Caenorhabditis elegans Reproductive Tract. PLoS Genetics, 9(1), e1003271. https://doi.org/10.1371/journal.pgen.1003271
    Tungjai, M., Whorton, E. B., & Rithidech, K. N. (2013). Persistence of apoptosis and inflammatory responses in the heart and bone marrow of mice following whole-body exposure to 28Silicon (28Si) ions. Radiation and Environmental Biophysics, 52(3), 339–350. https://doi.org/10.1007/s00411-013-0479-4
    Vedantham, V., Evangelista, M., Huang, Y., & Srivastava, D. (2013). Spatiotemporal regulation of an Hcn4 enhancer defines a role for Mef2c and HDACs in cardiac electrical patterning. Developmental Biology, 373(1), 149–162. https://doi.org/10.1016/j.ydbio.2012.10.017
    Veltrop, M., van der Kaa, J., Claassens, J., van Vliet, L., Verbeek, S., & Aartsma-Rus, A. (2013). Generation of Embryonic Stem Cells and Mice for Duchenne Research. PLoS Currents. https://doi.org/10.1371/currents.md.cbf1d33001de80923ce674302cad7925
    Hanstein, R., Negoro, H., Patel, N. K., Charollais, A., Meda, P., Spray, D. C., Suadicani, S. O., & Scemes, E. (2013). Promises and pitfalls of a Pannexin1 transgenic mouse line. Frontiers in Pharmacology, 4. https://doi.org/10.3389/fphar.2013.00061
    Chen, H., Hwang, H., McKee, L. A. K., Perez, J. N., Regan, J. A., Constantopoulos, E., LaFleur, B., & Konhilas, J. P. (2013). Temporal and morphological impact of pressure overload in transgenic FHC mice. Frontiers in Physiology, 4. https://doi.org/10.3389/fphys.2013.00205
    Chaker, B., Samra, T. A., Datta, N. S., & Abou-Samra, A. B. (2013). Altered Responses to Cold Environment in Urocortin 1 and Corticotropin-Releasing Factor Deficient Mice. Physiology Journal, 2013, 1–7. https://doi.org/10.1155/2013/185767
    Leontieva, O. V., Paszkiewicz, G. M., & Blagosklonny, M. V. (2012). Mechanistic or mammalian target of rapamycin (mTOR) may determine robustness in young male mice at the cost of accelerated aging. Aging, 4(12), 899–916.
    Austin, W. R., Armijo, A. L., Campbell, D. O., Singh, A. S., Hsieh, T., Nathanson, D., Herschman, H. R., Phelps, M. E., Witte, O. N., Czernin, J., & Radu, C. G. (2012). Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress. Journal of Experimental Medicine, 209(12), 2215–2228. https://doi.org/10.1084/jem.20121061
    Hecker, P. A., Mapanga, R. F., Kimar, C. P., Ribeiro, R. F., Brown, B. H., O’Connell, K. A., Cox, J. W., Shekar, K. C., Asemu, G., Essop, M. F., & Stanley, W. C. (2012). Effects of glucose-6-phosphate dehydrogenase deficiency on the metabolic and cardiac responses to obesogenic or high-fructose diets. AJP: Endocrinology and Metabolism, 303(8), E959–E972. https://doi.org/10.1152/ajpendo.00202.2012
    Yang, J., & Xu, X. (2012). Actinin2 is required for the lateral alignment of Z discs and ventricular chamber enlargement during zebrafish cardiogenesis. The FASEB Journal, 26(10), 4230–4242. https://doi.org/10.1096/fj.12-207969
    Kumar, M., Roe, K., Nerurkar, P. V., Namekar, M., Orillo, B., Verma, S., & Nerurkar, V. R. (2012). Impaired Virus Clearance, Compromised Immune Response and Increased Mortality in Type 2 Diabetic Mice Infected with West Nile Virus. PLoS ONE, 7(8), e44682. https://doi.org/10.1371/journal.pone.0044682

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