Intestine Tissue Homogenizer & Homogenization Protocol

Ideal for Intestine Tissue Homogenization

Do you spend lots of time and effort homogenizing intestine tissue samples? The Bullet Blender® is a multi-sample homogenizer that delivers superior results. No other homogenizer comes close to delivering the Bullet Blender’s winning combination of top-quality performance and budget-friendly affordability. See below for an intestine tissue homogenization protocol.

The Bullet Blender® Homogenizer
Save Time, Effort and Get Superior Results

  • Consistent and High Yield Results Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g.
  • No Cross Contamination No part of the Bullet Blender® ever touches the intestine samples – the sample tubes are kept closed during homogenization. There are no probes to clean between samples.
  • Samples Stay Cool Homogenizing causes only a few degrees of heating. Our “Blue” model comes with a fan to maintain ambient temperatures.
  • Easy and Convenient to Use Just place beads and buffer along with your intestine sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start.
  • Risk Free Purchase The Bullet Blender® comes with a 30 day money back guarantee and a 2 year warranty, with a 3 year warranty on the motor. The simple, reliable design enables the Bullet Blenders to sell for a fraction of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.
Bullet Blender Homogenizer

Bullet Blender Homogenization Protocol for Intestine Tissue

Sample size

See the Protocol

microcentrifuge tube model (up to 300 mg) Small intestine samples
5mL tube model (100mg – 1g) Medium intestine samples
 

Selected Publications for Intestinal Tissue

See all of our Bullet Blender publications!

Lei, S., Ryu, J., Wen, K., Twitchell, E., Bui, T., Ramesh, A., … Yuan, L. (2016). Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency. Scientific Reports, 6. https://doi.org/10.1038/srep25222
Lei, S., Samuel, H., Twitchell, E., Bui, T., Ramesh, A., Wen, K., … Yuan, L. (2016). Enterobacter cloacae inhibits human norovirus infectivity in gnotobiotic pigs. Scientific Reports, 6. https://doi.org/10.1038/srep25017
Bennike, T. B., Kastaniegaard, K., Padurariu, S., Gaihede, M., Birkelund, S., Andersen, V., & Stensballe, A. (2016). Proteome stability analysis of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human colon mucosal biopsies. Data in Brief, 6, 942–947. https://doi.org/10.1016/j.dib.2016.01.061
Bennike, T. B., Kastaniegaard, K., Padurariu, S., Gaihede, M., Birkelund, S., Andersen, V., & Stensballe, A. (2016). Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples. EuPA Open Proteomics, 10, 9–18. https://doi.org/10.1016/j.euprot.2015.10.001
Park, W. C., Kim, H.-R., Kang, D. B., Ryu, J.-S., Choi, K.-H., Lee, G.-O., … Park, D.-S. (2016). Comparative expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1 in gastric and colorectal cancer. BMC Cancer, 16, 358. https://doi.org/10.1186/s12885-016-2387-x
Falendysz, E. A., Lopera, J. G., Lorenzsonn, F., Salzer, J. S., Hutson, C. L., Doty, J., … Rocke, T. E. (2015). Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PLOS Neglected Tropical Diseases, 9(10), e0004130. https://doi.org/10.1371/journal.pntd.0004130
Capitán-Cañadas, F., Ocón, B., Aranda, C. J., Anzola, A., Suárez, M. D., Zarzuelo, A., … Martínez-Augustin, O. (2015). Fructooligosaccharides exert intestinal anti-inflammatory activity in the CD4+ CD62L+ T cell transfer model of colitis in C57BL/6J mice. European Journal of Nutrition. https://doi.org/10.1007/s00394-015-0962-6
Everman, J. L., Eckstein, T. M., Roussey, J., Coussens, P., Bannantine, J. P., & Bermudez, L. E. (2015). Characterization of the inflammatory phenotype of Mycobacterium avium subspecies paratuberculosis using a novel cell culture passage model. Microbiology, 161(7), 1420–1434. https://doi.org/10.1099/mic.0.000106
Danan-Gotthold, M., Golan-Gerstl, R., Eisenberg, E., Meir, K., Karni, R., & Levanon, E. Y. (2015). Identification of recurrent regulated alternative splicing events across human solid tumors. Nucleic Acids Research, 43(10), 5130–5144. https://doi.org/10.1093/nar/gkv210
Choong, E., Guo, J., Persson, A., Virding, S., Johansson, I., Mkrtchian, S., & Ingelman-Sundberg, M. (2015). Developmental Regulation and Induction of Cytochrome P450 2W1, an Enzyme Expressed in Colon Tumors. PLOS ONE, 10(4), e0122820. https://doi.org/10.1371/journal.pone.0122820
Zhou, Y., Rychahou, P., Wang, Q., Weiss, H. L., & Evers, B. M. (2015). TSC2/mTORC1 signaling controls Paneth and goblet cell differentiation in the intestinal epithelium. Cell Death and Disease, 6(2), e1631. https://doi.org/10.1038/cddis.2014.588
Doldur-Balli, F., Ozel, M. N., Gulsuner, S., Tekinay, A. B., Ozcelik, T., Konu, O., & Adams, M. M. (2015). Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ). BMC Neuroscience, 16(1). https://doi.org/10.1186/s12868-015-0229-4
Janocko, L., Althouse, A. D., Brand, R. M., Cranston, R. D., & McGowan, I. (2015). The Molecular Characterization of Intestinal Explant HIV Infection Using Polymerase Chain Reaction-Based Techniques. AIDS Research and Human Retroviruses, 31(10), 981–991. https://doi.org/10.1089/aid.2015.0165
Wang, Y.-M., Chai, S. C., Lin, W., Chai, X., Elias, A., Wu, J., … Chen, T. (2015). Serine 350 of human pregnane X receptor is crucial for its heterodimerization with retinoid X receptor alpha and transactivation of target genes in vitro and in vivo. Biochemical Pharmacology, 96(4), 357–368. https://doi.org/10.1016/j.bcp.2015.06.018
Eriksson, A., Williams, M. J., Voisin, S., Hansson, I., Krishnan, A., Philippot, G., … Schiöth, H. B. (2015). Implication of coronin 7 in body weight regulation in humans, mice and flies. BMC Neuroscience, 16(1), 13. https://doi.org/10.1186/s12868-015-0151-9
Liu, G., Yan, T., Fang, T., Jia, G., Chen, X., Zhao, H., … Wu, C. (2015). Nutrimetabolomic analysis provides new insights into spermine-induced ileum-system alterations for suckling rats. RSC Adv., 5(60), 48769–48778. https://doi.org/10.1039/C5RA01507C
Goodrich, K. M., Dorenkott, M. R., Ye, L., O’Keefe, S. F., Hulver, M. W., & Neilson, A. P. (2014). Dietary Supplementation with Cocoa Flavanols Does Not Alter Colon Tissue Profiles of Native Flavanols and Their Microbial Metabolites Established during Habitual Dietary Exposure in C57BL/6J Mice. Journal of Agricultural and Food Chemistry, 62(46), 11190–11199. https://doi.org/10.1021/jf503838q
Mapes, B., Chase, M., Hong, E., Ludvik, A., Ceryes, K., Huang, Y., & Kupfer, S. S. (2014). Ex vivo culture of primary human colonic tissue for studying transcriptional responses to 1 ,25(OH)2 and 25(OH) vitamin D. Physiological Genomics, 46(8), 302–308. https://doi.org/10.1152/physiolgenomics.00194.2013
van der Plas-Duivesteijn, S. J., Mohammed, Y., Dalebout, H., Meijer, A., Botermans, A., Hoogendijk, J. L., … Palmblad, M. (2014). Identifying Proteins in Zebrafish Embryos Using Spectral Libraries Generated from Dissected Adult Organs and Tissues. Journal of Proteome Research, 13(3), 1537–1544. https://doi.org/10.1021/pr4010585
Mascaraque, C., Aranda, C., Ocón, B., Monte, M. J., Suárez, M. D., Zarzuelo, A., … de Medina, F. S. (2014). Rutin has intestinal antiinflammatory effects in the CD4+ CD62L+ T cell transfer model of colitis. Pharmacological Research, 90, 48–57. https://doi.org/10.1016/j.phrs.2014.09.005
Borschensky, C. M., & Reinacher, M. (2014). Mutations in the 3c and 7b genes of feline coronavirus in spontaneously affected FIP cats. Research in Veterinary Science, 97(2), 333–340. https://doi.org/10.1016/j.rvsc.2014.07.016
Heinzerling, N. P., Liedel, J. L., Welak, S. R., Fredrich, K., Biesterveld, B. E., Pritchard, K. A., & Gourlay, D. M. (2014). Intestinal alkaline phosphatase is protective to the preterm rat pup intestine. Journal of Pediatric Surgery, 49(6), 954–960. https://doi.org/10.1016/j.jpedsurg.2014.01.031
Goodrich, K. M., & Neilson, A. P. (2014). Simultaneous UPLC–MS/MS analysis of native catechins and procyanidins and their microbial metabolites in intestinal contents and tissues of male Wistar Furth inbred rats. Journal of Chromatography B, 958, 63–74. https://doi.org/10.1016/j.jchromb.2014.03.011
Melero, M., García-Párraga, D., Corpa, J., Ortega, J., Rubio-Guerri, C., Crespo, J., … Sánchez-Vizcaíno, J. (2014). First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens). BMC Veterinary Research, 10(1), 968. https://doi.org/10.1186/s12917-014-0308-2
Naba, A., Clauser, K. R., Whittaker, C. A., Carr, S. A., Tanabe, K. K., & Hynes, R. O. (2014). Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver. BMC Cancer, 14(1), 518. https://doi.org/10.1186/1471-2407-14-518
Kovalenko, P. L., Yuan, L., Sun, K., Kunovska, L., Seregin, S., Amalfitano, A., & Basson, M. D. (2013). Regulation of Epithelial Differentiation in Rat Intestine by Intraluminal Delivery of an Adenoviral Vector or Silencing RNA Coding for Schlafen 3. PLoS ONE, 8(11), e79745. https://doi.org/10.1371/journal.pone.0079745
Rubio-Guerri, C., Melero, M., Esperón, F., Bellière, E., Arbelo, M., Crespo, J., … Sánchez-Vizcaíno, J. (2013). Unusual striped dolphin mass mortality episode related to cetacean morbillivirus in the Spanish Mediterranean sea. BMC Veterinary Research, 9(1), 106. https://doi.org/10.1186/1746-6148-9-106
Araújo-Pérez, F., McCoy, A. N., Okechukwu, C., Carroll, I. M., Smith, K. M., Jeremiah, K., … Keku, T. O. (2012). Differences in microbial signatures between rectal mucosal biopsies and rectal swabs. Gut Microbes, 3(6), 530–535. https://doi.org/10.4161/gmic.22157
Kovalenko, P. L., Flanigan, T. L., Chaturvedi, L., & Basson, M. D. (2012). Influence of defunctionalization and mechanical forces on intestinal epithelial wound healing. AJP: Gastrointestinal and Liver Physiology, 303(10), G1134–G1143. https://doi.org/10.1152/ajpgi.00321.2012
Kovalenko, P. L., Kunovska, L., Chen, J., Gallo, K. A., & Basson, M. D. (2012). Loss of MLK3 signaling impedes ulcer healing by modulating MAPK signaling in mouse intestinal mucosa. AJP: Gastrointestinal and Liver Physiology, 303(8), G951–G960. https://doi.org/10.1152/ajpgi.00158.2012
Forester, S. C., Gu, Y., & Lambert, J. D. (2012). Inhibition of starch digestion by the green tea polyphenol, (−)-epigallocatechin-3-gallate. Molecular Nutrition & Food Research, 56(11), 1647–1654. https://doi.org/10.1002/mnfr.201200206
Kovalenko, P. L., & Basson, M. D. (2012). Changes in morphology and function in small intestinal mucosa after Roux-en-Y surgery in a rat model. Journal of Surgical Research, 177(1), 63–69. https://doi.org/10.1016/j.jss.2012.03.011
Shaw, A. L., van Ginkel, F. W., Macklin, K. S., & Blake, J. P. (2011). Effects of phytase supplementation in broiler diets on a natural Eimeria challenge in naive and vaccinated birds. Poultry Science, 90(4), 781–790. https://doi.org/10.3382/ps.2010-01158
Lowe, E. L., Crother, T. R., Rabizadeh, S., Hu, B., Wang, H., Chen, S., … Arditi, M. (2010). Toll-Like Receptor 2 Signaling Protects Mice from Tumor Development in a Mouse Model of Colitis-Induced Cancer. PLoS ONE, 5(9), e13027. https://doi.org/10.1371/journal.pone.0013027
Lancaster, K. Z., & Pfeiffer, J. K. (2010). Limited Trafficking of a Neurotropic Virus Through Inefficient Retrograde Axonal Transport and the Type I Interferon Response. PLoS Pathogens, 6(3), e1000791. https://doi.org/10.1371/journal.ppat.1000791
Lu, J., & Grenache, D. G. (2010). High-throughput tissue homogenization method and tissue-based quality control materials for a clinical assay of the intestinal disaccharidases. Clinica Chimica Acta, 411(9–10), 754–757. https://doi.org/10.1016/j.cca.2010.02.009
Xiao, L., Cao, W., Liu, G., Fang, T., Wu, X., Jia, G., … Cai, J. (n.d.). Arginine, N-carbamylglutamate, and glutamine exert protective effects against oxidative stress in rat intestine. Animal Nutrition. https://doi.org/10.1016/j.aninu.2016.04.005
Jensen, A. R., Manning, M. M., Khaneki, S., Drucker, N. A., & Markel, T. A. (n.d.). Harvest tissue source does not alter the protective power of stromal cell therapy after intestinal ischemia and reperfusion injury. Journal of Surgical Research. https://doi.org/10.1016/j.jss.2016.05.006
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