Lung Tissue Homogenizer & Homogenization Protocol

Ideal for Lung Tissue Homogenization

Do you spend lots of time and effort homogenizing lung tissue samples? The Bullet Blender® is a multi-sample homogenizer that delivers superior results. No other homogenizer comes close to delivering the Bullet Blender’s winning combination of top-quality performance and budget-friendly affordability. See below for a lung tissue homogenization protocol.

The Bullet Blender® Homogenizer
Save Time, Effort and Get Superior Results

  • Consistent and High Yield Results Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g.
  • No Cross Contamination No part of the Bullet Blender® ever touches the lung samples – the sample tubes are kept closed during homogenization. There are no probes to clean between samples.
  • Samples Stay Cool Homogenizing causes only a few degrees of heating. Our “Blue” model comes with a fan to maintain ambient temperatures.
  • Easy and Convenient to Use Just place beads and buffer along with your lung sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start.
  • Risk Free Purchase The Bullet Blender® comes with a 30 day money back guarantee and a 2 year warranty, with a 3 year warranty on the motor. The simple, reliable design enables the Bullet Blenders to sell for a fraction of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.
Bullet Blender Homogenizer

Bullet Blender Homogenization Protocol for Lung Tissue

Sample size

See the Protocol

microcentrifuge tube model (up to 300 mg) Small lung samples
5mL tube model (100mg – 1g) Medium lung samples
50mL tube model (100mg – 3.5g) Large lung samples
 

Selected Publications for Lung Tissue

See all of our Bullet Blender publications!

Li, M., Li, C., Song, S., Kang, H., Yang, D., & Li, G. (2016). Development and antigenic characterization of three recombinant proteins with potential for Glässer’s disease prevention. Vaccine, 34(19), 2251–2258. https://doi.org/10.1016/j.vaccine.2016.03.014
Adamo, R. F., Fishbein, I., Zhang, K., Wen, J., Levy, R. J., Alferiev, I. S., & Chorny, M. (2016). Magnetically enhanced cell delivery for accelerating recovery of the endothelium in injured arteries. Journal of Controlled Release, 222, 169–175. https://doi.org/10.1016/j.jconrel.2015.12.025
Bawadekar, M., Gendron-Fitzpatrick, A., Rebernick, R., Shim, D., Warner, T. F., Nicholas, A. P., … Shelef, M. A. (2016). Tumor necrosis factor alpha, citrullination, and peptidylarginine deiminase 4 in lung and joint inflammation. Arthritis Research & Therapy, 18, 173. https://doi.org/10.1186/s13075-016-1068-0
Hajkova, K., Jurasek, B., Sykora, D., Palenicek, T., Miksatkova, P., & Kuchar, M. (2015). Salting-out-assisted liquid–liquid extraction as a suitable approach for determination of methoxetamine in large sets of tissue samples. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-015-9221-1
Ruhaak, L. R., Taylor, S. L., Stroble, C., Nguyen, U. T., Parker, E. A., Song, T., … Miyamoto, S. (2015). Differential N-Glycosylation Patterns in Lung Adenocarcinoma Tissue. Journal of Proteome Research, 14(11), 4538–4549. https://doi.org/10.1021/acs.jproteome.5b00255
Falendysz, E. A., Lopera, J. G., Lorenzsonn, F., Salzer, J. S., Hutson, C. L., Doty, J., … Rocke, T. E. (2015). Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PLOS Neglected Tropical Diseases, 9(10), e0004130. https://doi.org/10.1371/journal.pntd.0004130
McKenna, S., Gossling, M., Bugarini, A., Hill, E., Anderson, A. L., Rancourt, R. C., … Wright, C. J. (2015). Endotoxemia Induces I B /NF- B-Dependent Endothelin-1 Expression in Hepatic Macrophages. The Journal of Immunology, 195(8), 3866–3879. https://doi.org/10.4049/jimmunol.1501017
Marien, E., Meister, M., Muley, T., Fieuws, S., Bordel, S., Derua, R., … Swinnen, J. V. (2015). Non-small cell lung cancer is characterized by dramatic changes in phospholipid profiles: Altered Phospholipid Profiles in NSCLC. International Journal of Cancer, 137(7), 1539–1548. https://doi.org/10.1002/ijc.29517
Otsubo, C., Bharathi, S., Uppala, R., Ilkayeva, O. R., Wang, D., McHugh, K., … Goetzman, E. S. (2015). Long-chain Acylcarnitines Reduce Lung Function by Inhibiting Pulmonary Surfactant. Journal of Biological Chemistry, 290(39), 23897–23904. https://doi.org/10.1074/jbc.M115.655837
Rosen, D. A., Hilliard, J. K., Tiemann, K. M., Todd, E. M., Morley, S. C., & Hunstad, D. A. (2015). Klebsiella pneumoniae FimK Promotes Virulence in Murine Pneumonia. Journal of Infectious Diseases, jiv440. https://doi.org/10.1093/infdis/jiv440
Danan-Gotthold, M., Golan-Gerstl, R., Eisenberg, E., Meir, K., Karni, R., & Levanon, E. Y. (2015). Identification of recurrent regulated alternative splicing events across human solid tumors. Nucleic Acids Research, 43(10), 5130–5144. https://doi.org/10.1093/nar/gkv210
Kim, B. S., Serebreni, L., Fallica, J., Hamdan, O., Wang, L., Johnston, L., … Hassoun, P. M. (2015). Cyclin-Dependent Kinase Five Mediates Activation of Lung Xanthine Oxidoreductase in Response to Hypoxia. PLOS ONE, 10(4), e0124189. https://doi.org/10.1371/journal.pone.0124189
Caverly, L. J., Caceres, S. M., Fratelli, C., Happoldt, C., Kidwell, K. M., Malcolm, K. C., … Nichols, D. P. (2015). Mycobacterium abscessus Morphotype Comparison in a Murine Model. PLOS ONE, 10(2), e0117657. https://doi.org/10.1371/journal.pone.0117657
Diaz, J., Warren, L., Helfner, L., Xue, X., Chatterjee, P. K., Gupta, M., … Metz, C. N. (2015). Obesity shifts house dust mite-induced airway cellular infiltration from eosinophils to macrophages: effects of glucocorticoid treatment. Immunologic Research, 63(1–3), 197–208. https://doi.org/10.1007/s12026-015-8717-2
Khademi, S., Frye, M. A., Jeckel, K. M., Schroeder, T., Monnet, E., Irwin, D. C., … Hamilton, K. L. (2015). Hypoxia mediated pulmonary edema: potential influence of oxidative stress, sympathetic activation and cerebral blood flow. BMC Physiology, 15(1). https://doi.org/10.1186/s12899-015-0018-4
Hilliard, K. L., Allen, E., Traber, K. E., Kim, Y., Wasserman, G. A., Jones, M. R., … Quinton, L. J. (2015). Activation of Hepatic STAT3 Maintains Pulmonary Defense during Endotoxemia. Infection and Immunity, 83(10), 4015–4027. https://doi.org/10.1128/IAI.00464-15
Tian, X., Liu, M., Su, X., Jiang, Z., Ma, Q., Liao, X., … Zhou, R. (2015). Mapping the epitope of neutralizing monoclonal antibodies against human adenovirus type 3. Virus Research, 208, 66–72. https://doi.org/10.1016/j.virusres.2015.06.002
Lopera, J. G., Falendysz, E. A., Rocke, T. E., & Osorio, J. E. (2015). Attenuation of monkeypox virus by deletion of genomic regions. Virology, 475, 129–138. https://doi.org/10.1016/j.virol.2014.11.009
Eriksson, A., Williams, M. J., Voisin, S., Hansson, I., Krishnan, A., Philippot, G., … Schiöth, H. B. (2015). Implication of coronin 7 in body weight regulation in humans, mice and flies. BMC Neuroscience, 16(1), 13. https://doi.org/10.1186/s12868-015-0151-9
Chen, G.-S., Huang, K.-F., Huang, C.-C., & Wang, J.-Y. (2015). Thaliporphine Derivative Improves Acute Lung Injury after Traumatic Brain Injury. BioMed Research International, 2015, 1–10. https://doi.org/10.1155/2015/729831
Zhang, Z., He, L., Hu, S., Wang, Y., Lai, Q., Yang, P., … Wang, C.-Y. (2015). AAL exacerbates pro-inflammatory response in macrophages by regulating Mincle/Syk/Card9 signaling along with the Nlrp3 inflammasome assembly. American Journal of Translational Research, 7(10), 1812–1825.
Hyatt, L. D., Wasserman, G. A., Rah, Y. J., Matsuura, K. Y., Coleman, F. T., Hilliard, K. L., … Jones, M. R. (2014). Myeloid ZFP36L1 Does Not Regulate Inflammation or Host Defense in Mouse Models of Acute Bacterial Infection. PLoS ONE, 9(10), e109072. https://doi.org/10.1371/journal.pone.0109072
Rose, S. J., Neville, M. E., Gupta, R., & Bermudez, L. E. (2014). Delivery of Aerosolized Liposomal Amikacin as a Novel Approach for the Treatment of Nontuberculous Mycobacteria in an Experimental Model of Pulmonary Infection. PLoS ONE, 9(9), e108703. https://doi.org/10.1371/journal.pone.0108703
Miller, M., Rosenthal, P., Beppu, A., Mueller, J. L., Hoffman, H. M., Tam, A. B., … Broide, D. H. (2014). ORMDL3 Transgenic Mice Have Increased Airway Remodeling and Airway Responsiveness Characteristic of Asthma. The Journal of Immunology, 192(8), 3475–3487. https://doi.org/10.4049/jimmunol.1303047
Goetzman, E. S., Alcorn, J. F., Bharathi, S. S., Uppala, R., McHugh, K. J., Kosmider, B., … Vockley, J. (2014). Long-chain Acyl-CoA Dehydrogenase Deficiency as a Cause of Pulmonary Surfactant Dysfunction. Journal of Biological Chemistry, 289(15), 10668–10679. https://doi.org/10.1074/jbc.M113.540260
Bailey, K. L., Bonasera, S. J., Wilderdyke, M., Hanisch, B. W., Pavlik, J. A., DeVasure, J., … Wyatt, T. A. (2014). Aging causes a slowing in ciliary beat frequency, mediated by PKC. AJP: Lung Cellular and Molecular Physiology, 306(6), L584–L589. https://doi.org/10.1152/ajplung.00175.2013
Neely, C. J., Kartchner, L. B., Mendoza, A. E., Linz, B. M., Frelinger, J. A., Wolfgang, M. C., … Cairns, B. A. (2014). Flagellin Treatment Prevents Increased Susceptibility to Systemic Bacterial Infection after Injury by Inhibiting Anti-Inflammatory IL-10+ IL-12- Neutrophil Polarization. PLoS ONE, 9(1), e85623. https://doi.org/10.1371/journal.pone.0085623
Borschensky, C. M., & Reinacher, M. (2014). Mutations in the 3c and 7b genes of feline coronavirus in spontaneously affected FIP cats. Research in Veterinary Science, 97(2), 333–340. https://doi.org/10.1016/j.rvsc.2014.07.016
Goosen, W. J., Miller, M. A., Chegou, N. N., Cooper, D., Warren, R. M., van Helden, P. D., & Parsons, S. D. C. (2014). Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer). Veterinary Immunology and Immunopathology, 160(1–2), 133–138. https://doi.org/10.1016/j.vetimm.2014.03.015
Melero, M., García-Párraga, D., Corpa, J., Ortega, J., Rubio-Guerri, C., Crespo, J., … Sánchez-Vizcaíno, J. (2014). First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens). BMC Veterinary Research, 10(1), 968. https://doi.org/10.1186/s12917-014-0308-2
Ivanov, S. S., & Roy, C. R. (2013). Pathogen signatures activate a ubiquitination pathway that modulates the function of the metabolic checkpoint kinase mTOR. Nature Immunology, 14(12), 1219–1228. https://doi.org/10.1038/ni.2740
Yamamoto, K., Ahyi, A.-N. N., Pepper-Cunningham, Z. A., Ferrari, J. D., Wilson, A. A., Jones, M. R., … Mizgerd, J. P. (2013). Roles of Lung Epithelium in Neutrophil Recruitment During Pneumococcal Pneumonia. American Journal of Respiratory Cell and Molecular Biology, 130906140418002. https://doi.org/10.1165/rcmb.2013-0114OC
Karauzum, H., Adhikari, R. P., Sarwar, J., Devi, V. S., Abaandou, L., Haudenschild, C., … Aman, M. J. (2013). Structurally Designed Attenuated Subunit Vaccines for S. aureus LukS-PV and LukF-PV Confer Protection in a Mouse Bacteremia Model. PLoS ONE, 8(6), e65384. https://doi.org/10.1371/journal.pone.0065384
Chorny, M., Fishbein, I., Tengood, J. E., Adamo, R. F., Alferiev, I. S., & Levy, R. J. (2013). Site-specific gene delivery to stented arteries using magnetically guided zinc oleate-based nanoparticles loaded with adenoviral vectors. The FASEB Journal, 27(6), 2198–2206. https://doi.org/10.1096/fj.12-224659
Nakayama, K. H., Lee, C. C. I., Batchelder, C. A., & Tarantal, A. F. (2013). Tissue Specificity of Decellularized Rhesus Monkey Kidney and Lung Scaffolds. PLoS ONE, 8(5), e64134. https://doi.org/10.1371/journal.pone.0064134
Moghadaszadeh, B., Rider, B. E., Lawlor, M. W., Childers, M. K., Grange, R. W., Gupta, K., … Beggs, A. H. (2013). Selenoprotein N deficiency in mice is associated with abnormal lung development. The FASEB Journal, 27(4), 1585–1599. https://doi.org/10.1096/fj.12-212688
Al-Hasan, Y. M., Evans, L. C., Pinkas, G. A., Dabkowski, E. R., Stanley, W. C., & Thompson, L. P. (2013). Chronic Hypoxia Impairs Cytochrome Oxidase Activity Via Oxidative Stress in Selected Fetal Guinea Pig Organs. Reproductive Sciences, 20(3), 299–307. https://doi.org/10.1177/1933719112453509
Hoang, H. D., Prasain, J. K., Dorand, D., & Miller, M. A. (2013). A Heterogeneous Mixture of F-Series Prostaglandins Promotes Sperm Guidance in the Caenorhabditis elegans Reproductive Tract. PLoS Genetics, 9(1), e1003271. https://doi.org/10.1371/journal.pgen.1003271
Nichols, D. P., Caceres, S., Caverly, L., Fratelli, C., Kim, S. H., Malcolm, K., … Nick, J. A. (2013). Effects of azithromycin in Pseudomonas aeruginosa burn wound infection. Journal of Surgical Research, 183(2), 767–776. https://doi.org/10.1016/j.jss.2013.02.003
Rubio-Guerri, C., Melero, M., Esperón, F., Bellière, E., Arbelo, M., Crespo, J., … Sánchez-Vizcaíno, J. (2013). Unusual striped dolphin mass mortality episode related to cetacean morbillivirus in the Spanish Mediterranean sea. BMC Veterinary Research, 9(1), 106. https://doi.org/10.1186/1746-6148-9-106
Lee, J., Brehm, M. A., Greiner, D., Shultz, L. D., & Kornfeld, H. (2013). Engrafted human cells generate adaptive immune responses to Mycobacterium bovis BCG infection in humanized mice. BMC Immunology, 14(1), 53. https://doi.org/10.1186/1471-2172-14-53
Austin, W. R., Armijo, A. L., Campbell, D. O., Singh, A. S., Hsieh, T., Nathanson, D., … Radu, C. G. (2012). Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress. Journal of Experimental Medicine, 209(12), 2215–2228. https://doi.org/10.1084/jem.20121061
Hong, N., De-Xing, Q., Deng-Xiang, Z., & Long-Zhong, R. (2012). A Simple Cultural Method for Detection of Mycoplasma bovis. Journal of Animal and Veterinary Advances, 11(10), 1643–1646. https://doi.org/10.3923/javaa.2012.1643.1646
Kumar, M., Roe, K., Nerurkar, P. V., Namekar, M., Orillo, B., Verma, S., & Nerurkar, V. R. (2012). Impaired Virus Clearance, Compromised Immune Response and Increased Mortality in Type 2 Diabetic Mice Infected with West Nile Virus. PLoS ONE, 7(8), e44682. https://doi.org/10.1371/journal.pone.0044682
Sanderson, S. D., Thoman, M. L., Kis, K., Virts, E. L., Herrera, E. B., Widmann, S., … Phillips, J. A. (2012). Innate Immune Induction and Influenza Protection Elicited by a Response-Selective Agonist of Human C5a. PLoS ONE, 7(7), e40303. https://doi.org/10.1371/journal.pone.0040303
Quinton, L. J., Mizgerd, J. P., Hilliard, K. L., Jones, M. R., Kwon, C. Y., & Allen, E. (2012). Leukemia Inhibitory Factor Signaling Is Required for Lung Protection during Pneumonia. The Journal of Immunology, 188(12), 6300–6308. https://doi.org/10.4049/jimmunol.1200256
El Agha, E., Al Alam, D., Carraro, G., MacKenzie, B., Goth, K., De Langhe, S. P., … Bellusci, S. (2012). Characterization of a Novel Fibroblast Growth Factor 10 (Fgf10) Knock-In Mouse Line to Target Mesenchymal Progenitors during Embryonic Development. PLoS ONE, 7(6), e38452. https://doi.org/10.1371/journal.pone.0038452
Adhikari, R. P., Karauzum, H., Sarwar, J., Abaandou, L., Mahmoudieh, M., Boroun, A. R., … Aman, M. J. (2012). Novel Structurally Designed Vaccine for S. aureus α-Hemolysin: Protection against Bacteremia and Pneumonia. PLoS ONE, 7(6), e38567. https://doi.org/10.1371/journal.pone.0038567
Cavasin, M. A., Demos-Davies, K., Horn, T. R., Walker, L. A., Lemon, D. D., Birdsey, N., … McKinsey, T. A. (2012). Selective Class I Histone Deacetylase Inhibition Suppresses Hypoxia-Induced Cardiopulmonary Remodeling Through an Antiproliferative Mechanism. Circulation Research, 110(5), 739–748. https://doi.org/10.1161/CIRCRESAHA.111.258426
Konsavage, W. M., Zhang, L., Wu, Y., & Shenberger, J. S. (2012). Hyperoxia-induced activation of the integrated stress response in the newborn rat lung. AJP: Lung Cellular and Molecular Physiology, 302(1), L27–L35. https://doi.org/10.1152/ajplung.00174.2011
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