Spleen Tissue Homogenizer & Homogenization Protocol

Ideal for Spleen Tissue Homogenization

Do you spend lots of time and effort homogenizing spleen tissue samples? The Bullet Blender® tissue homogenizer delivers high quality and superior yields. No other homogenizer comes close to delivering the Bullet Blender’s winning combination of top-quality performance and budget-friendly affordability. See below for a spleen tissue homogenization protocol.

Save Time, Effort and Get Superior Results with

The Bullet Blender Homogenizer

Consistent and High Yield Results

Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g.

No Cross Contamination

No part of the Bullet Blender ever touches the tissue – the sample tubes are kept closed during homogenization. There are no probes to clean between samples.

Samples Stay Cool

The Bullet Blenders’ innovative and elegant design provides convective cooling of the samples, so they do not heat up more than several degrees. In fact, our Gold+ models hold the sample temperature to about 4ºC.

Easy and Convenient to Use

Just place beads and buffer along with your tissue sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start.

Risk Free Purchase

Thousands of peer-reviewed journal articles attest to the consistency and quality of the Bullet Blender homogenizer. We offer a 2 year warranty, extendable to 4 years, because our Bullet Blenders are reliable and last for many years.  

Spleen Tissue Homogenization Protocol

Sample size

See the Protocol
microcentrifuge tube model (up to 300 mg) Small spleen samples
5mL tube model (100mg - 1g) Medium spleen samples
50mL tube model (100mg - 3.5g) Large spleen samples

What Else Can You Homogenize? Tough or Soft, No Problem! 

The Bullet Blender can process a wide range of samples including organ tissue, cell culture, plant tissue, and small organisms. You can homogenize samples as tough as mouse femur or for gentle applications such as tissue dissociation or organelle isolation.

the Bullet Blender high-throughput tissue homogenizer

Spleen tissue pieces (on beads in upper photo) are completely homogenized into the buffer (darker in lower photo). 

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    Bullet Blender Models

    Select Publications using the Bullet Blender to Homogenize Spleen Tissue

    See all of our Bullet Blender publications!

    Li, M., Li, C., Song, S., Kang, H., Yang, D., & Li, G. (2016). Development and antigenic characterization of three recombinant proteins with potential for Glässer’s disease prevention. Vaccine, 34(19), 2251–2258. https://doi.org/10.1016/j.vaccine.2016.03.014
    Cole, L. E., Zhang, J., Kesselly, A., Anosova, N. G., Lam, H., Kleanthous, H., & Yethon, J. A. (2016). Limitations of Murine Models for Assessment of Antibody-Mediated Therapies or Vaccine Candidates against Staphylococcus epidermidis Bloodstream Infection. Infection and Immunity, 84(4), 1143–1149. https://doi.org/10.1128/IAI.01472-15
    Adamo, R. F., Fishbein, I., Zhang, K., Wen, J., Levy, R. J., Alferiev, I. S., & Chorny, M. (2016). Magnetically enhanced cell delivery for accelerating recovery of the endothelium in injured arteries. Journal of Controlled Release, 222, 169–175. https://doi.org/10.1016/j.jconrel.2015.12.025
    Falendysz, E. A., Lopera, J. G., Lorenzsonn, F., Salzer, J. S., Hutson, C. L., Doty, J., Gallardo-Romero, N., Carroll, D. S., Osorio, J. E., & Rocke, T. E. (2015). Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PLOS Neglected Tropical Diseases, 9(10), e0004130. https://doi.org/10.1371/journal.pntd.0004130
    Rosen, D. A., Hilliard, J. K., Tiemann, K. M., Todd, E. M., Morley, S. C., & Hunstad, D. A. (2015). Klebsiella pneumoniae FimK Promotes Virulence in Murine Pneumonia. Journal of Infectious Diseases, jiv440. https://doi.org/10.1093/infdis/jiv440
    Liao, X., Ren, J., Wei, C.-H., Ross, A. C., Cecere, T. E., Jortner, B. S., Ahmed, S. A., & Luo, X. M. (2015). Paradoxical Effects of All-Trans-Retinoic Acid on Lupus-Like Disease in the MRL/lpr Mouse Model. PLOS ONE, 10(3), e0118176. https://doi.org/10.1371/journal.pone.0118176
    Caverly, L. J., Caceres, S. M., Fratelli, C., Happoldt, C., Kidwell, K. M., Malcolm, K. C., Nick, J. A., & Nichols, D. P. (2015). Mycobacterium abscessus Morphotype Comparison in a Murine Model. PLOS ONE, 10(2), e0117657. https://doi.org/10.1371/journal.pone.0117657
    Palomares, R. A., Sakamoto, K., Walz, H. L., Brock, K. V., & Hurley, D. J. (2015). Acute infection with bovine viral diarrhea virus of low or high virulence leads to depletion and redistribution of WC1+ γδ T cells in lymphoid tissues of beef calves. Veterinary Immunology and Immunopathology, 167(3–4), 190–195. https://doi.org/10.1016/j.vetimm.2015.07.016
    Neishabouri, S. H., Hutson, S. M., & Davoodi, J. (2015). Chronic activation of mTOR complex 1 by branched chain amino acids and organ hypertrophy. Amino Acids, 47(6), 1167–1182. https://doi.org/10.1007/s00726-015-1944-y
    Lopera, J. G., Falendysz, E. A., Rocke, T. E., & Osorio, J. E. (2015). Attenuation of monkeypox virus by deletion of genomic regions. Virology, 475, 129–138. https://doi.org/10.1016/j.virol.2014.11.009
    Eriksson, A., Williams, M. J., Voisin, S., Hansson, I., Krishnan, A., Philippot, G., Yamskova, O., Herisson, F. M., Dnyansagar, R., Moschonis, G., Manios, Y., Chrousos, G. P., Olszewski, P. K., Frediksson, R., & Schiöth, H. B. (2015). Implication of coronin 7 in body weight regulation in humans, mice and flies. BMC Neuroscience, 16(1), 13. https://doi.org/10.1186/s12868-015-0151-9
    Zhang, Z., He, L., Hu, S., Wang, Y., Lai, Q., Yang, P., Yu, Q., Zhang, S., Xiong, F., Simsekyilmaz, S., Ning, Q., Li, J., Zhang, D., Zhang, H., Xiang, X., Zhou, Z., Sun, H., & Wang, C.-Y. (2015). AAL exacerbates pro-inflammatory response in macrophages by regulating Mincle/Syk/Card9 signaling along with the Nlrp3 inflammasome assembly. American Journal of Translational Research, 7(10), 1812–1825.
    Hyatt, L. D., Wasserman, G. A., Rah, Y. J., Matsuura, K. Y., Coleman, F. T., Hilliard, K. L., Pepper-Cunningham, Z. A., Ieong, M., Stumpo, D. J., Blackshear, P. J., Quinton, L. J., Mizgerd, J. P., & Jones, M. R. (2014). Myeloid ZFP36L1 Does Not Regulate Inflammation or Host Defense in Mouse Models of Acute Bacterial Infection. PLoS ONE, 9(10), e109072. https://doi.org/10.1371/journal.pone.0109072
    Bannantine, J. P., Everman, J. L., Rose, S. J., Babrak, L., Katani, R., Barletta, R. G., Talaat, A. M., Grohn, Y. T., Chang, Y.-F., Kapur, V., & Bermudez, L. E. (2014). Evaluation of eight live attenuated vaccine candidates for protection against challenge with virulent Mycobacterium avium subspecies paratuberculosis in mice. Frontiers in Cellular and Infection Microbiology, 4. https://doi.org/10.3389/fcimb.2014.00088
    Palomares, R. A., Hurley, D. J., Woolums, A. R., Parrish, J. E., & Brock, K. V. (2014). Analysis of mRNA expression for genes associated with regulatory T lymphocytes (CD25, FoxP3, CTLA4, and IDO) after experimental infection with bovine viral diarrhea virus of low or high virulence in beef calves. Comparative Immunology, Microbiology and Infectious Diseases, 37(5–6), 331–338. https://doi.org/10.1016/j.cimid.2014.10.001
    Palomares, R. A., Parrish, J., Woolums, A. R., Brock, K. V., & Hurley, D. J. (2014). Expression of toll-like receptors and co-stimulatory molecules in lymphoid tissue during experimental infection of beef calves with bovine viral diarrhea virus of low and high virulence. Veterinary Research Communications, 38(4), 329–335. https://doi.org/10.1007/s11259-014-9613-2
    Borschensky, C. M., & Reinacher, M. (2014). Mutations in the 3c and 7b genes of feline coronavirus in spontaneously affected FIP cats. Research in Veterinary Science, 97(2), 333–340. https://doi.org/10.1016/j.rvsc.2014.07.016
    Palomares, R. A., Brock, K. V., & Walz, P. H. (2014). Differential expression of pro-inflammatory and anti-inflammatory cytokines during experimental infection with low or high virulence bovine viral diarrhea virus in beef calves. Veterinary Immunology and Immunopathology, 157(3–4), 149–154. https://doi.org/10.1016/j.vetimm.2013.12.002
    Melero, M., García-Párraga, D., Corpa, J., Ortega, J., Rubio-Guerri, C., Crespo, J., Rivera-Arroyo, B., & Sánchez-Vizcaíno, J. (2014). First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens). BMC Veterinary Research, 10(1), 968. https://doi.org/10.1186/s12917-014-0308-2
    Erickson, A. K., & Pfeiffer, J. K. (2013). Dynamic Viral Dissemination in Mice Infected with Yellow Fever Virus Strain 17D. Journal of Virology, 87(22), 12392–12397. https://doi.org/10.1128/JVI.02149-13
    Wiles, T. J., Norton, J. P., Russell, C. W., Dalley, B. K., Fischer, K. F., & Mulvey, M. A. (2013). Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen. PLoS Genetics, 9(8), e1003716. https://doi.org/10.1371/journal.pgen.1003716
    Silver, A. C., Dunne, D. W., Zeiss, C. J., Bockenstedt, L. K., Radolf, J. D., Salazar, J. C., & Fikrig, E. (2013). MyD88 Deficiency Markedly Worsens Tissue Inflammation and Bacterial Clearance in Mice Infected with Treponema pallidum, the Agent of Syphilis. PLoS ONE, 8(8), e71388. https://doi.org/10.1371/journal.pone.0071388
    Karauzum, H., Adhikari, R. P., Sarwar, J., Devi, V. S., Abaandou, L., Haudenschild, C., Mahmoudieh, M., Boroun, A. R., Vu, H., Nguyen, T., Warfield, K. L., Shulenin, S., & Aman, M. J. (2013). Structurally Designed Attenuated Subunit Vaccines for S. aureus LukS-PV and LukF-PV Confer Protection in a Mouse Bacteremia Model. PLoS ONE, 8(6), e65384. https://doi.org/10.1371/journal.pone.0065384
    Chorny, M., Fishbein, I., Tengood, J. E., Adamo, R. F., Alferiev, I. S., & Levy, R. J. (2013). Site-specific gene delivery to stented arteries using magnetically guided zinc oleate-based nanoparticles loaded with adenoviral vectors. The FASEB Journal, 27(6), 2198–2206. https://doi.org/10.1096/fj.12-224659
    Nichols, D. P., Caceres, S., Caverly, L., Fratelli, C., Kim, S. H., Malcolm, K., Poch, K. R., Saavedra, M., Solomon, G., Taylor-Cousar, J., Moskowitz, S., & Nick, J. A. (2013). Effects of azithromycin in Pseudomonas aeruginosa burn wound infection. Journal of Surgical Research, 183(2), 767–776. https://doi.org/10.1016/j.jss.2013.02.003
    Palomares, R. A., Walz, H. G., & Brock, K. V. (2013). Expression of type I interferon-induced antiviral state and pro-apoptosis markers during experimental infection with low or high virulence bovine viral diarrhea virus in beef calves. Virus Research, 173(2), 260–269. https://doi.org/10.1016/j.virusres.2013.02.010
    Poulsen, K. P., Faith, N. G., Steinberg, H., & Czuprynski, C. J. (2013). Bacterial load and inflammation in fetal tissues is not dependent on IL-17a or IL-22 in 10–14 day pregnant mice infected with Listeria monocytogenes. Microbial Pathogenesis, 56, 47–52. https://doi.org/10.1016/j.micpath.2012.11.003
    Hanstein, R., Negoro, H., Patel, N. K., Charollais, A., Meda, P., Spray, D. C., Suadicani, S. O., & Scemes, E. (2013). Promises and pitfalls of a Pannexin1 transgenic mouse line. Frontiers in Pharmacology, 4. https://doi.org/10.3389/fphar.2013.00061
    Austin, W. R., Armijo, A. L., Campbell, D. O., Singh, A. S., Hsieh, T., Nathanson, D., Herschman, H. R., Phelps, M. E., Witte, O. N., Czernin, J., & Radu, C. G. (2012). Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress. Journal of Experimental Medicine, 209(12), 2215–2228. https://doi.org/10.1084/jem.20121061
    Kumar, M., Roe, K., Nerurkar, P. V., Namekar, M., Orillo, B., Verma, S., & Nerurkar, V. R. (2012). Impaired Virus Clearance, Compromised Immune Response and Increased Mortality in Type 2 Diabetic Mice Infected with West Nile Virus. PLoS ONE, 7(8), e44682. https://doi.org/10.1371/journal.pone.0044682
    Adhikari, R. P., Karauzum, H., Sarwar, J., Abaandou, L., Mahmoudieh, M., Boroun, A. R., Vu, H., Nguyen, T., Devi, V. S., Shulenin, S., Warfield, K. L., & Aman, M. J. (2012). Novel Structurally Designed Vaccine for S. aureus α-Hemolysin: Protection against Bacteremia and Pneumonia. PLoS ONE, 7(6), e38567. https://doi.org/10.1371/journal.pone.0038567
    Bessen, R. A., Robinson, C. J., Seelig, D. M., Watschke, C. P., Lowe, D., Shearin, H., Martinka, S., & Babcock, A. M. (2011). Transmission of Chronic Wasting Disease Identifies a Prion Strain Causing Cachexia and Heart Infection in Hamsters. PLoS ONE, 6(12), e28026. https://doi.org/10.1371/journal.pone.0028026
    Smith, J. S., Xu, Z., Tian, J., Palmer, D. J., Ng, P., & Byrnes, A. P. (2011). The Role of Endosomal Escape and Mitogen-Activated Protein Kinases in Adenoviral Activation of the Innate Immune Response. PLoS ONE, 6(10), e26755. https://doi.org/10.1371/journal.pone.0026755
    Gogal, Jr., R. M., Kerr, R., Kingsley, D. H., Granata, L. A., LeRoith, T., Holliman, S. D., Dancho, B. A., & Flick, Jr., G. J. (2011). High Hydrostatic Pressure Processing of Murine Norovirus 1–Contaminated Oysters Inhibits Oral Infection in STAT-1<SUP>-/-</SUP>–Deficient Female Mice. Journal of Food Protection, 74(2), 209–214. https://doi.org/10.4315/0362-028X.JFP-10-235
    Rayner, K. J., Suarez, Y., Davalos, A., Parathath, S., Fitzgerald, M. L., Tamehiro, N., Fisher, E. A., Moore, K. J., & Fernandez-Hernando, C. (2010). MiR-33 Contributes to the Regulation of Cholesterol Homeostasis. Science, 328(5985), 1570–1573. https://doi.org/10.1126/science.1189862
    Lancaster, K. Z., & Pfeiffer, J. K. (2010). Limited Trafficking of a Neurotropic Virus Through Inefficient Retrograde Axonal Transport and the Type I Interferon Response. PLoS Pathogens, 6(3), e1000791. https://doi.org/10.1371/journal.ppat.1000791

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