Complete Lysis and Extraction Kit
The PrecisionPak™ offers a streamlined, complete solution for very high quality and high yield biomolecule extractions from many sample types. The PrecisionPak™ bundles together the bead lysis kits with the extraction kits and an optimized, step-by-step protocol, specific for your sample. It also includes additional reagents that you would typically have to purchase separately for higher quality biomolecules, such as a reagent to digest DNA from RNA samples and one to remove unwanted proteins. This means that all the necessary components to homogenize tissue samples and isolate specific biomolecules from the homogenates, and achieve excellent results are now available in one single package!
The lysis kits in the PrecisionPak are available in 1.5 mL screw cap and snap-cap tubes for Bullet Blender homogenizers, and 2 mL screw cap tubes compatible with most other brands of homogenizers. The extraction kits are selected for high quality results and ease of use. For example the RNA and DNA kits use magnetic bead technology and avoid organic solvents. The custom protocols are optimized and specific for your specific sample, to make it easy for you to obtain great results.
- Superior Yield and Quality
- Tailored Protocols
- Comprehensive Kit
- Save Time
- No Organic Solvents
- Great Value
Each PrecisionPak includes Lysis and Extraction Kits, a custom protocol and additional reagents that normally users need to obtain separately for improving quality.
Why Choose the PrecisionPak™?
Superior Yield and Quality
The magnetic bead technology isolates high quantities of nucleic acid. Because of the high surface area of the beads in suspension, more biomolecules are able to bind. Unlike a packed column, the free-floating beads mix with the nucleic acids in the homogenate where maximum binding can occur.
Additional reagents for high quality yields, that you typically need to purchaes separately are included in the kits: Proteinase K, RNase A (in DNA isolation kit), DNase I (in RNA isolation kit), and FoamBlocker. The result is high quality DNA or RNA product.
Comparison of PrecisionPak MAGneat DNA extraction kit and “Qompetitor” kit. Purified DNA from 30mg of various tissue samples were extracted either using PrecisionPak MAGneat DNA kit or “Qompetitor” kit, following manufacturer’s instructions. Extracted DNA samples were run on a 1% Agarose gel. M – 1 kb ladder.
PCR amplification from DNA isolated using PrecisionPak MAGneat Animal tissue kit. PCR amplification was performed to amplify a 900bp fragment of ACT1 gene. PCR products were run on a 1% Agarose gel. M – 100bp ladder.
Comparison of PrecisionPak MAGneat RNA extraction kit, “Qompetitor” kit and Trizol extraction method. Purified RNA from 30mg of various tissue samples or whole Drosophila were extracted using PrecisionPak MAGneat RNA or “Qompetitor” extraction kit, following manufacturer’s instructions or using the Trizol extraction method. Extracted RNA samples were analyzed using the Agilent 2100 Bioanalyzer. L – Ladder
Optimized Protocols
We provide protocols that have been developed and tested for your specific application. Bullet Blender® speed and time settings are optimized to avoid DNA degradation for high quality product. In-house data is available and reflects the quality of the kits. See our sample protocols for Mouse Bone – PrecisionPak DNA Lysis and Extraction Protocol and Drosophila – PrecisionPak RNA Lysis and Extraction Protocol!
Comprehensive Kits
The PrecisionPak provides everything you need: all the buffers and reagents and the protocol for high quality and high yield. The PrecisionPak includes additional enzymes such as Proteinase K to facilitate tissue digestion and RNase A (in DNA extraction kit) to remove the contaminating RNA and DNase I (in RNA extraction kit) to remove the contaminating DNA. The kits also include an optimized protocol.
Save Time
Use of magnetic beads instead of spin columns eliminates the need of multiple centrifugation steps, which is both tedious and time-consuming. Our magnetic rack system enables you to batch process up to 8 samples in a rack. The magnetic field can be applied for wash steps and removed for binding steps, without the samples tubes ever leaving the rack. This saves time and provides convenience.Download Additional Information
- Download the PrecisionPak Brochure
Select a Kit
Animal Tissue
Adipose, Adrenal Gland, Aorta, Bladder, Blood Vessel, Bone, Brain, Cartilage, Cecum, Colon, Cranium, Diaphragm, Duodenum, Ear, Epithelium, Eye, Feces, Fish Organs, Gallbladder, Genital Warts, Heart, Hypothalamus, Intestinal Mucosa, Intestine, Jaw, Jejunum, Kidney, Larynx, Liver, Lung, Lymph Node, Marrow, Meconium, Mouse Femur and Tibia, Muscle, Nasal, Olfactory Mucosa, Oropharynx, Pancreas, Peritoneal Macrophages, Pharynx, Pituitary Gland, Placenta, Polyp, Salivary Gland, Skin, Spleen, Stomach, Tail Snips, Teeth, Testes, Thalamus, Thymus Gland, Thyroid Gland, Tongue, Trachea, Tumor, Umbilical Cord, Uterus, Wound Tissue
Caterpillar, Crayfish, Crickets, Fruit Fly (Drosophila), Honeybee, Hookworm, Mosquito, Nematode (B. xylophilus), Plankton, Roundworm (C. elegans), Shrimp, Ticks, Zebrafish (D. rerio), Zooplankton, Xenopus Tissue
Plants, Microorganisms
The range of kits is expanding rapidly, with kits for isolating proteins and kits for plants and microorganisms coming soon.
Tube Sizes
Kits are available for the Bullet Blender in:
- 1.5 mL snap cap GATOR™ or Eppendorf® tubes
- 1.5 mL screw cap RINO® tubes
and for other brand homogenizers in:
- 2 mL screw cap tubes
Questions? Need Assistance?
Small Organism kits
Use our magnetic rack system for quick and easy extractions.
