Lysis and Extraction Protocols

Before you start. Please read the General Guidelines before using the Bullet Blender® in any application.

Download our User Guide. It contains instructions on using the Bullet Blender, preparing samples including procedures for special cases, bead selection, and special considerations.

Need help? E-mail our tech support at [email protected] and we’ll do our best to assist you.

Share protocols! If you have developed a protocol with the Bullet Blender that is not listed on this page, please share it with everyone! E-mail it to us at [email protected] or send it using our support form and we’ll review it and add it to our list of protocols.

PLEASE NOTE: The protocols used with the Bullet Blender are different than protocols used with other bead-mill homogenizers. Protocols for other bead-mill homogenizers will not work with the Bullet Blender. Does the protocol use a different kind of bead or bead lysis kit than what you currently have? Click here for pricing and ordering.

Animal tissues

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 HomogenizationExtraction
 Small-size samples up to 0.3 gMedium-size samples up to 1 gLarge-size samples up to 3.5 gDNARNA
Aortic tissueHomogenizationHomogenization
BladderHomogenizationHomogenizationHomogenization
Blood (dried)Homogenization
Blood (fresh)Homogenization
Blood vesselsHomogenizationHomogenization
BonesHomogenizationDNA
BrainHomogenizationHomogenizationHomogenizationDNARNA
Cerebrospinal fluidHomogenization
Ear punchHomogenizationDNA
EyeHomogenizationHomogenizationHomogenizationDNA
Fat or Adipose tissueHomogenizationHomogenizationHomogenization
FemurHomogenizationHomogenization
Gall bladderHomogenizationHomogenizationHomogenization
HeartHomogenizationHomogenizationHomogenizationDNARNA
IntestineHomogenizationHomogenizationDNA
KidneyHomogenizationHomogenizationHomogenizationDNARNA
LiverHomogenizationHomogenizationHomogenizationDNARNA
LungHomogenizationHomogenizationHomogenizationDNA —
Lymph tissuesHomogenizationHomogenization
MusclesHomogenizationHomogenizationHomogenizationDNA
PancreasHomogenizationHomogenizationHomogenization
PharynxHomogenizationHomogenization
SkinHomogenizationHomogenizationDNA
Spinal columnHomogenization
SpleenHomogenizationHomogenizationHomogenizationDNA
StomachHomogenizationHomogenizationDNA
Tail snipsHomogenizationHomogenization
Toe clipsDNA
TestisHomogenizationHomogenization
ThymusHomogenizationHomogenization
ThyroidHomogenizationHomogenization
TracheaHomogenizationHomogenization
Tumor tissuesHomogenizationHomogenization
Umbilical cordHomogenizationHomogenization
UterusHomogenizationHomogenizationHomogenization

Plant tissues (including fruits, grains, and seeds)

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  LeavesRootsStemSeeds
AlfalfaHomogenization
AlmondsHomogenization
Arabidopsis thalianaHomogenizationHomogenizationHomogenization
Barberry Homogenization
BarleyHomogenization
BlueberryHomogenization
CitrusHomogenization
Conifer needles (lyophilized)Homogenization
Corn (Dried)Homogenization
Corn (Frozen)Homogenization
Cuminum cyminumHomogenization
Fruit tree twigsHomogenization
Fungal sporesHomogenization
GingerHomogenization
HorseradishHomogenization
LicoriceHomogenization
Malanga tuberHomogenization
Mung beansHomogenization
MushroomHomogenization
MustardHomogenization
NematanthusHomogenization
Nigella sativaHomogenization
OakHomogenization
OnionHomogenization
PeachHomogenization
Pine nutsHomogenization
RiceHomogenization
RoseHomogenization
SoybeanHomogenizationHomogenization
StrawberryHomogenization
Sugar cane pulpHomogenization
TomatoHomogenizationHomogenization
Wheat berriesHomogenization

Single cell organisms (Mammalian cell culture, Yeast, Bacteria)

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 Small-size samples    up to 0.3 gMedium-size samples up to 1 gLarge-size samples up to 3.5 g
CHO-K1
Escherischia coliHomogenizationHomogenizationHomogenization
HeLa
Mammalian cell cultureHomogenizationHomogenizationHomogenization
Yeast (Candida)HomogenizationHomogenizationHomogenization
Yeast – BY4741
Yeast – YA244

Small organisms (insects, fish, nematodes)

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 HomogenizationExtraction
 WholeLarvaeDNARNA
Butterflies
Caenorhabditis elegansHomogenizationDNARNA
Drosophila melanogasterHomogenizationDNARNA
Herring
Mosquitoes
Shellfish
Ticks
ZebrafishHomogenization

Medicines, hair, powders, etc.

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FeathersHomogenization
HairHomogenization
PillsHomogenization

Soils and sediments

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Deep sea sedimentHomogenization
Garden soil

Body excretions (Feces, Saliva, Nasal swabs, etc.)

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Feces
MeconiumHomogenization
Nasal swabs
Saliva
Semen

Live cell isolation (mammalian cells/bacteria/virus isolation)

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 Isolated from
Bacteria
Mammalian cells
Tumor cells
Virus