“An optimal flow rate for CO2 euthanasia systems should displace 10% to 30% of the chamber or cage volume/min. Prefilled chambers are unacceptable.“ – American Veterinary Medical Association (2013) AVMA Guidelines for the Euthanasia of Animals: 2013 Edition, pg. 49
“Gradual-fill CO2 exposure causes aversion in rodents beginning at approximately a 15% concentration and lasting to onset of unconsciousness. If an appropriate gradual displacement rate is used, animals will lose consciousness before CO2 concentrations become painful. A 20%/min gradual displacement produces unconsciousness in 106 seconds at a CO2 concentration of 30%; a slower 10%/min displacement increases time to onset of unconsciousness to 156 seconds at a CO2 concentration of 21%.“ – American Veterinary Medical Association (2013) AVMA Guidelines for the Euthanasia of Animals: 2013 Edition, pg. 25
The AVMA Guidelines can be found here: https://www.avma.org/KB/Policies/Documents/euthanasia.pdf
*Applicable to mice, rats, hamsters, guinea pigs, gerbils, degus and cotton rats
Compliance made easy with the Quietek Euthanization system. Learn more!