Bullet Blender® Protocols

Our experienced staff of molecular biologists have worked to provide you with a set of optimized protocols for the homogenization of various tissue, cell types, and organisms so you can spend less time troubleshooting and more time getting results.

All of our currently available protocols are listed below. Please read the General Guidelines before using the Bullet Blender® in any application.

For helpful suggestions, download our Reference Manual. It contains instructions on using the Bullet Blender, preparing samples including procedures for special cases, bead selection, and special considerations.

If you need help developing an assay with the Bullet Blender, feel free to e-mail our tech support at support@NextAdvance.com and we’ll do our best to assist you.

If you have developed a protocol with the Bullet Blender that is not listed on this page, or you have developed a protocol that works better for certain applications, please share it with everyone! E-mail it to us at support@NextAdvance.com or send it using our support form and we’ll review it and add it to our list of protocols. Don’t worry – we’ll acknowledge you for it! Thanks!

PLEASE NOTE: The protocols used with the Bullet Blender are different than protocols used with other bead-mill homogenizers. If you previously used another product, please be sure not to use its protocols with the Bullet Blender, as you will very likely experience different results.

Does the protocol use a different kind of bead than the type you currently have? Click here for pricing and ordering.

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Searches can be combined. If a protocol is available, it will appear in the box on the right.

Browse all protocols

Animal tissues

Sample nameMicrocentrifuge tube models5 mL tube models50 mL tube models
Aortic tissueSmall samplesMedium-size samples
BladderSmall samplesMedium-size samplesLarge samples
Blood (dried)Small samples
Blood (fresh)Small samples
Blood vesselsSmall samplesMedium-size samples
BrainSmall samplesMedium-size samplesLarge samples
Cancer tissue (carcinoma)Small samplesMedium-size samples
Cerebrospinal fluidSmall samples
Ear punchSmall samples
EpitheliumSmall samplesMedium-size samples
EyeSmall samplesMedium-size samplesLarge samples
Fat or Adipose TissueSmall samplesMedium-size samplesLarge samples
GallbladderSmall samplesMedium-size samplesLarge samples
HeartSmall samplesMedium-size samplesLarge samples
IntestineSmall samplesMedium-size samples
KidneySmall samplesMedium-size samplesLarge samples
LiverSmall samplesMedium-size samplesLarge samples
LungSmall samplesMedium-size samplesLarge samples
Lymphatic tissueSmall samplesMedium-size samples
MeconiumSmall samplesMedium-size samplesLarge samples
Mouse FemurSmall samplesMedium-size samples
Muscle (Striated)Small samplesMedium-size samplesLarge samples
Organs (for bacterial extraction)
Extract bacteria/virus/organelles
Small samples
PancreasSmall samplesMedium-size samplesLarge samples
PharynxSmall samplesMedium-size samples
SkinSmall samplesMedium-size samples
Skin (Pig)Small samplesMedium-size samples
SpleenSmall samplesMedium-size samplesLarge samples
Spleen
Live cell suspension
Small samples
StomachSmall samplesMedium-size samples
Tail snipSmall samplesMedium-size samples
TestisSmall samplesMedium-size samples
ThymusSmall samplesMedium-size samples
ThyroidSmall samplesMedium-size samples
TracheaSmall samplesMedium-size samples
Umbilical cordSmall samplesMedium-size samples
UterusSmall samplesMedium-size samplesLarge samples

Plant tissues (other than grains or seeds)

Sample nameMicrocentrifuge tube models5 mL tube models50 mL tube models
Arabidopsis thalianaSmall samples
Barberry (leaves)Small samples
BlueberrySmall samples
Citrus leavesLarge samples
Conifer needles (lyophilized)Small samples
Corn Kernels Medium-size samples
Fruit tree twigsMedium-size samples
Fungal sporesSmall samples
Ginger (Rhizome)Small samples
Horseradish (Root)Small samples
Leaves (lyophilized, small)Small samples
Leek (Leaves)Small samples
Licorice root (dry)Small samples
Malanga (tuber)Small samples
MushroomSmall samples
Nematanthus (leaves)Small samples
Oak (mature leaves)Large samples
Peach (fruit)Large samples
Seeds (Onion)Medium-size samples
Seeds (Tomato)Medium-size samples
Soybean (fresh)Small samples
Soybean stems (fresh)Medium-size samples
Sugar cane pulpSmall samples
Tomato (root)Small samples

Small organisms

Sample nameMicrocentrifuge tube models5 mL tube models50 mL tube models
Drosophila S2Small samples
Fruit fly (adult)Small samplesMedium-size samples
Fruit fly (larva)Small samplesMedium-size samples
Insects (large soft-bodied)Small samples
Insects (light and hard)Small samples
Nematode (C. elegans)Small samplesMedium-size samples
Shellfish (General)Large samples
Zebrafish (Larva)Small samplesMedium-size samples

Cell culture

Sample nameMicrocentrifuge tube models5 mL tube models50 mL tube models
Escherischia coliSmall samplesMedium-size samplesLarge samples
Mammalian cell cultureSmall samplesMedium-size samplesLarge samples
Yeast (Candida)Small samplesMedium-size samplesLarge samples
Yeast (Saccharomyces)Small samplesMedium-size samplesLarge samples

Dried grains, seeds, etc.

Sample nameMicrocentrifuge tube models5 mL tube models50 mL tube models
Alfalfa seedsMedium-size samples
Almonds (shelled)Medium-size samples
Barley grainsMedium-size samples
Corn kernels (dried, dent corn)Medium-size samples
Mung beans Medium-size samples
Onion (seed) Medium-size samples
Pine nutSmall samples
Rice grains (dried hulled)Small samples
Sap (dried)Large samples
Soybean (dried)Medium-size samples
Spelt grainsSmall samples
Sunflower seeds (with shells)Medium-size samples
Tomato (seed)Medium-size samples
Wheat berriesSmall samples

Other samples

Sample nameMicrocentrifuge tube models5 mL tube models50 mL tube models
Feathers (vanes and down)Medium-size samples
HairSmall samplesMedium-size samples
Pills (tablets)Large samples
Powdered compoundsSmall samples
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