We’re proud to offer comprehensive support for the CheckitĀ® product line.
- Lifetime technical support. We’re always happy to help you get the best results from your Pipette Checkit Pro or Checkit Go. Just call +1 518 674 3510 or email [email protected]
Instructions
- Pipette Checkit Pro Instructions for measuring the accuracy of single-channel pipettes.
- Checkit Go Instructions for measuring the accuracy of liquid handling robots and pipettes.
The CheckitĀ® Pro for evaluating single-channel pipettes
Instructions
Instructions on how to use the Pipette Checkit in PDF format.
Additional Information
Calibration vs. verification
The Pipette Checkit® is a tool to identify potential volume delivery errors for pipettes. It is not a calibration tool. If your institution requires pipette calibration on a specific schedule, you should adhere to this schedule and use the Checkit
as an extra verification step between scheduled calibrations.
Expected performance at different volumes
Piston pipettes are most accurate at or near their nominal volume (the maximum volume the pipette can deliver). When a pipette is used near the bottom of its range, the measured volumes will vary more, and are also more strongly affected by slight variations
in pipetting style or environmental factors like humidity. The markings on the Checkit reflect this variation.
A note on environment: One advantage of the Checkit is that it can be used in the same room in which the experiment will be conducted. This can help identify variation in delivered volume due to pressure, humidity, or temperature differences
between the calibration environment and the experimental environment. Because the Checkit directly measures delivered volume rather than relying on gravimetric analysis, there is also no need to use correction calculations that account for these factors.
Accuracy and precision
Most pipette manufacturers provide specifications for both the accuracy and precision of their pipettes. Briefly, accuracy describes how close the mean of delivered volumes is to the stated volume, and precision describes the distribution of the volumes
around the mean. If you choose to compare your Checkit readings to the manufacturerās specifications, remember to account for both accuracy and precision when determining what range is acceptable.
Note that ISO 8655 standards differ from the manufacturerās standards. If your pipette has been recalibrated since purchase, you should check with your calibration service to determine which standards were used, and consider comparing your Checkit readings
to that standard rather than to the manufacturerās standard.
Useful links
The CheckitĀ® Go for robotic liquid handlers and multi-channel pipettes
Instructions
Instructions on how to use the Checkit Go in PDF format.
Labware Definitions and Interface Drawing
We have a list, below, of labware definitions and liquid classes, supplied by robot manufacturers. This list is rapidly growing. We also have an interface drawing showing the dimensions of the CheckitĀ® Go to help you program your robot if a labware definition has not been created yet.
Feel free to reach out to us if you would like help. +1 518 674 3510 or [email protected]
Interface drawing
Click for the interface drawing with dimensions of the Checkit Go, to help you program your robot, if a labware definition is not available yet.
Labware definitions
Beckman Coulter Life Sciences
A step-by-step guide for creating the Next Advance Checkit Go labware definition for your Biomek i-Series Automated Liquid Handler.
Eppendorf
They ask that you contact them directly for the labware definition. Contact their customer support team at [email protected]
Formulatrix
To use Checkit with Formulatrix liquid handlers, the manufacturer recommends to use a standard SBS 96-well plate definition with the height set to 22.64mm. Run a protocol to aspirate the dye from column 1 and dispense to column 12 and flip the tab to validate the pipetting accuracy.
Genie Life Sciences
Labware definitions for the Checkit Go can be found in customer accounts for all Genie liquid handlers. See this video to find what the labware and operation will look like.
Hamilton
Opentrons
PerkinElmer
Download these .lab files and save them to C:\Packard\Janus\Labware Files folder location to use them in WinPrep.
Other brands coming soon
Videos
Watch an example of a Checkit being used on an Opentrons robot.