BlotBot Blot Processor


Throw out your timers! Don’t babysit your blots again!

The Freedom Rocker™ BlotBot® is an automated blot processor for incubating and washing blots and gels, after the transfer from gel to blot. Using a microprocessor for precise timing and dispensing, it automatically processes Westerns, Northerns, and Southerns in a consistent manner, every time. Ideal for unattended staining of gels, it frees you from babysitting your samples. Simply add your buffers and reagents, and the BlotBot carries out your existing protocol.

  • Consistent processing. Better results.
  • Plan your next experiment over coffee, not lunch.
  • Process blots unattended so you can focus on other tasks.
  • Use as little as 3.5 mL of antibody (or up to 50 mL of reagent) for each mini blot, and recover separately.
  • Large color LCD display for easy programming of protocols.

BlotBot vs Manual Western Blot

Automated Processor for Western Blots

Throw Out Your Timers

Are you chained to your timer? Throw it out and use the Freedom Rocker BlotBot. Simply place your blots, gels, or slides in trays, set the program, connect the bottles/tubes of reagents and walk away. The microcontroller in the Freedom Rocker BlotBot will ensure that the reagents and washes will be accurately pumped into and out of the trays at the precise times. Results will be consistent because all trays will receive the same reagent or wash buffer at the same time in equal volumes. Multi-dispense models can even dispense one different reagent to each tray, i.e. primary or secondary antibody.

Be productive and concentrate on another task, enjoy your lunch, OR let it run overnight!

Automated Processor for Western Blots
Consistent automated blot processing with the BlotBot.

western blot, processed / washed with Freedom Rocker
Western blot analysis of extracts from human squamous carcinoma cells using anti-myc antibody. Courtesy of Dr. Aparna Ranganathan, the University at Albany (SUNY).

Improve Consistency and Accuracy

By automating the processing of blots and gels, the Freedom Rocker BlotBot yields very consistent and accurate blots, gels, and slides. It automatically dispenses and aspirates equal volumes of reagents from multiple trays simultaneously. The samples in all the trays will experience the exact same protocol.

Shave Months Off Your Research

Complete the testing portion of your research project in half the time. Blots are ready when you arrive at work. You can review them over coffee, not lunch, so you can start your next test iteration the same day.

Increase Productivity

Automatically processing blots and gels cuts down on distractions. Instead of babysitting your blots you have more time to focus on other tasks, eat lunch, or attend meetings – or let it run overnight. Through increased productivity, the Freedom Rocker BlotBot pays for itself in about 30 uses.

The Freedom Rocker BlotBot replicates your existing protocols. There is no need to develop new protocols so you can start processing right away.

Risk Free Purchase

The Freedom Rocker BlotBot comes with a 30 day money back guarantee and a 2 year warranty. Customers have been using Freedom Rockers since 2005, and most of those sold in the first years remain in use today.

Download the Freedom Rocker™ BlotBot® brochure.

Cut Down on Distractions

Freedom Rocker Steps for western blot processing

Portion of a sample protocol – showing the addition and washing of one reagent using the Freedom Rocker BlotBot.

The Freedom Rocker BlotBot can be programmed to perform up to 3 of these reagent steps, and process your Western blots and gels unattended. Your blots can be ready to analyze when you arrive at work the following morning.

Automated Processor for Western Blots


“The BlotBot saved my life – not only does it save me a ton of time because I don’t have to babysit my blots while it’s processing, but the blots come out really clean and consistent every time.” Srividya Venkitachalam, Case Western Reserve University
“We are so excited about our new BlotBot that my students have asked me to acquire another one!” Dr. Karen Briski, University of Louisiana at Monroe
“The results from the BlotBot are higher quality and more consistent than manual blot processing. I love having the blots processed overnight, ready to image in the morning. Amazing instrument!” Nat Clark, University of Texas Health and Science Center at San Antonio
“Overall I am pretty impressed by the BlotBot, because so far the membranes came out clean and results are comparable to those generated by our own regular way.” Rachel Pong, University of Texas Southwestern Medical School
“I’m one of the first users of the Freedom Rocker at Amgen in Cambridge, MA. The machine gets LOTS of use from all of us here.” Dr. Barbara Grubinska, Amgen
“We love it! It saves us a lot of time.” Dr. Lilliane Roellard, Genentech
“It is perfect for silver staining of gels for mass spectrometry. It has made an UNBELIEVABLE impact on data generation for me in cancer protein screening.” Dr. Steve Griffiths, Atlantic Cancer Research Institute
“We are very pleased with the instrument.” Dr. Richard Ingram, Schering-Plough
“The Freedom Rocker is really convenient.” Dr. Michele Lastro, The UAlbany Gen*NY*Sis Center for Excellence in Cancer Genomics


Does manual processing of western blots produce inconsistent signals between repeats? BlotBot is the answer!

BlotBot produces consistent signal strength EVERY TIME with consistent wash volumes and wash times


  • Unattended dispensing and pouring off of liquids.
  • Only requires an electrical outlet – no vacuum, pressurized air or gas, or additional pumps required.
  • Can recover reagents, such as antibody solutions, separately from each gel or blot.
  • Trays can hold mini blots (maximum dimension 8cm x 8cm) and midi blots (maximum dimension 8cm x 16cm) and strip blots (maximum dimension 4cm x 8cm; Blotbot 248 and 248V).
  • Can switch between 2 wash buffers (BlotBot 248V only).
  • Adjustable Parameters for each protocol step include:
    • rocking speed (5 to 40 cycles per minute)
    • reagent/solution rocking time (1 min to 96 hours)
    • dispense volume (10 to 150 ml) up to 10 repetitions per step
    • clearing rinse option
  • Patented technology.
  • Rocking continues after the final rinse and the wash buffer remains in the trays to prevent the blots or gels from desiccating.
  • Built in the USA for years of trouble free use.
  • Uses existing protocols. Programming is easy and follows standard protocols steps. Programs can be up to 9 steps where each step is an immersion in a reagent (e.g. antibody) or a series of washes.
  • In each step reagents or buffer will be dispensed and aspirated to all the trays simultaneously, for consistent processing of all the blots and gels.
  • The minimum volume required to fully cover each mini blot is 3.5 mL.
  • A clearing rinse can be activated to provide a quick rinse to get the final drop of reagent after each wash or reagent immersion.
  • Quiet.
  • The removable trays can be washed. Replacements can be ordered separately.
  • A bottle of buffer is connected to the Freedom Rocker BlotBot with a hose and leak-proof quick-connector connections.
  • 30-day money-back guarantee.
  • Two year warranty.

3 Key Advantages

A small ammount of antibody passes over the blot as the BlotBot rocks
1. Use very small volumes of antibody solution.
Similar to blots in a rolling bottle, a small puddle of antibody passes repeatedly over the blot in curved bottom trays.

recovered waste
2. Recover reagents separately.
Reagents from each blot are captured in 50 mL tubes, ready for storage.

programing screen on the BlotBot
3. Use your existing protocols.
Each program step matches each step in your protocol. For example, a 3X 10 min. buffer wash is a 3X 10 min. buffer wash. No development effort required.
BlotBot Features
Automated Processor for Western Blots



Features and Capacities BlotBot 240 BlotBot 248 BlotBot 248V
Number of Blots up to 4 mini blots
or 2 midi blots
up to 8 strips
or 4 mini blots
or 2 midi blots
up to 8 strips
or 4 mini blots
or 2 midi blots
Wash Buffers 1 1 2
Buffer Volume
per compartment
Midi blot tray: 10 – 150 mL
Mini blot tray: 10 – 75 mL
Midi blot tray: 10 – 150 mL
Mini blot tray: 10 – 75 mL
Strip blot tray: 10 – 35 mL
Midi blot tray: 10 – 150 mL
Mini blot tray: 10 – 75 mL
Strip blot tray: 10 – 35 mL
Reagents (e.g. antibodies) per blot Dispense: 2
Recover: 2
Dispense: 2
Recover: 2
Dispense: 2
Recover: 2
Reagent volume up to 50 mL each up to 50 mL each up to 50 mL each
Minimum reagent per blot Mini: 3.5 mL (recover 3 mL)
Midi: 7 mL (recover 6 mL)
Strip: 2 mL (recover 1.5 mL)
Mini: 3.5 mL (recover 3 mL)
Midi: 7 mL (recover 6 mL)
Strip: 2 mL (recover 1.5 mL)
Mini: 3.5 mL (recover 3 mL)
Midi: 7 mL (recover 6 mL)
Protocol steps up to 9 up to 9 up to 9
Washes per step up to 10 up to 10 up to 10
Rocks per minute 5 to 40, independent rate for each step 5 to 40, independent rate for each step 5 to 40, independent rate for each step
Bench Footprint width: 20.75 in (53 cm)
depth: 15.25 in (39 cm)
height: 16.5 in (42 cm)
width: 20.75 in (53 cm)
depth: 15.25 in (39 cm)
height: 16.5 in (42 cm)
width: 20.75 in (53 cm)
depth: 15.25 in (39 cm)
height: 16.5 in (42 cm)
Features and Capacities BlotBot 240
Number of Blots up to 4 mini blots or up to 2 midi blots
Buffers 1
Buffer Volume Midi blot tray: 10 – 150 mL
Mini blot tray: 10 – 75 mL per side
Reagents per blot Dispense: 2
Recover: 2
Reagent volume up to 50 mL each
Minimum reagent per blot Mini blot: 3.5 mL (recover 3 mL)
Midi blot: 7 mL (recover 6 mL)
Protocol steps up to 9
Washes per step up to 10
Rocks per minute 5 to 40, independent rate for each step
Bench Footprint 20.75 in (52.7 cm) width, 15.25 in (38.7 cm) depth, 16.5 in (41.9 cm) height

BlotBot Models



The Freedom Rocker™ BlotBot® carries out the blocking, washing, and antibody incubation steps of your western blot for you. To carry out a western blot using the BlotBot:

  1. Place your transferred blot and reagents inside the BlotBot. Add your blot to the curved rocking tray, blocking buffer to the tray with the blot, wash buffer to the buffer bottle, and antibody solutions to the reagent tubes.
  2. Run a program. Either edit a program or run an existing program. You can use whatever protocol you normally use, up to nine steps. See the Programming tab for more details.
  3. Press play. Your blots or gels will be processed without any babysitting!
And remember… Automated Processor for Western Blots



The Freedom Rocker™ BlotBot® is designed to work with your existing protocols. To enter a protocol into the BlotBot, follow these steps:

  1. From the home screen, select “Programs”. Select one of the programs using the dial, and press the “Edit” button to edit the program. The BlotBot comes with 8 programs with default values.
  2. Choose a name for your protocol. The “Save” button saves changes, while the “Continue” button moves on to the next screen.
  3. Select whether the protocol will be used with a mini blot tray (2 chambers with a central divider), or a midi blot tray (one large chamber).
  4. Now, choose whether you want a clearing rinse to be included in the protocol. A clearing rinse will briefly rinse the blot with 10 mL of wash buffer at the end of each step.
  5. Select the speeds, times and reagents for each step in your protocol. When selecting the reagent, you can choose the following: “Buffer” from the buffer bottle; “1” or “2” from the reagent tubes; and “END” to indicate that there are no more steps. Buffer wash steps can be repeated up to 10 times; reagent steps cannot be repeated; reagents can be recovered. Because blots and gels should be kept wet, the first step assumes that there is liquid, such as a blocking buffer, in the tray, and after the final step, the buffer (or reagent) will remain in the tray, and the tray will continue to rock slowly. Press “Save” to save all of your changes.


Freedom Rocker™ BlotBot® FAQs

Can the Freedom Rocker BlotBot work in labs in other countries?

Yes. A wall transformer will convert the voltage from your wall outlet to 12V DC for the Freedom Rocker. Select the proper voltage when you order your instrument.

How much room does the BlotBot require?

The BlotBot 120 requires 15 inches (37 cm) of lab bench, models 240 and 248 require 21 inches (53 cm). The bottle for wash buffer can be placed behind or to the side of the BlotBot, or even on a shelf above or below the lab bench.

What is the minimum volume of reagent or antibody required?

Only 3.5 ml of antibody is required for a miniblot.

How do I switch between mini and midi size gels or blots?

The BlotBot comes with separate trays sized for mini and midi blots and gels. Model 248 also comes with trays for strip blots. Simply use the appropriate tray and set the blotsize in the program.

What happens at the end of the final wash step?

After the programmed duration of the final wash step, the rocking continues with the wash buffer in the tray(s).

What was the change in trays in 2019? Can newer trays fit in older BlotBots?

In 2019 we revised the trays and the tray holder to accommodate slightly wider blots (from 8 cm to 8.7 cm). Below is an image showing the differences. Older BlotBots can be retrofitted to handle wider blots by replacing the tray holder and trays with the newer items. Important Tray Differences

How many trays can the BlotBot run at once?

The BlotBot 120 can run 1 midi or 2 mini blots or gels at a time. The BlotBot 240 can run 2 midi or 4 mini blots or gels at a time. The BlotBot 248 can run 2 midi or 4 mini blots or gels or 8 strips at a time.

Do all the trays follow the same protocol?

Different reagents can be dispensed and collected from each blot or gel, however, the timing of each step and the volume of buffer in each wash step will be the same.

Can I dispense and recover different antibodies into each tray?

Yes, you can dispense different reagents for each blot, and the reagents can be recovered separately.

Can I reuse the reagent tubes?

You can either use a new reagent tube (RRVR-100 pack of 100 or RRVR-500 pack of 500) or replace the foil seal (RVRFL, pack of 200). If you are using a different antibody, we highly recommend using a new tube. If you are using the same antibody that was previously used in the reagent tube, and you want to save money you can replace the foil seal.

How do I replace the foil seal on a reagent tube?

To replace the foil seal, first completely remove the previous seal and make sure that the surface is completely clean and without cuts or nicks. Next, place a new seal on the bottom of the reagent tube and apply firm pressure all around. This process requires care to ensure that the seals do not leak. For more information, download the instructions for this process here.


Accessories for the BlotBot

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