Pipette Checkit

Overview

Verification on the spot




Why take chances with your sample analysis?

Evaluate the accuracy of your pipettes in seconds with our convenient Pipette Checkit™ cartridges. Low-cost, fast, and easy to use, the Checkit can save money on calibration services while giving you the confidence that your pipettes are delivering accurate volumes. No more trying to read a tiny droplet’s weight on a high-precision balance.

Whenever you need a fast, quantitative assessment of a pipette’s accuracy, just pull out a Checkit. Available for various size pipettes, the Checkit checks your pipette’s accuracy at the bottom, middle and top of its range. Wouldn’t it be nice to know whether a pipette needs recalibration, or if lab personnel could use a brush-up on their pipetting technique? With the Checkit, a clear answer is seconds away.

Many pipette manufacturers recommend regular in-laboratory checking of pipettes in addition to good maintenance and scheduled calibration. The Checkit provides a valuable early-warning system for a pipette that may be drifting out of calibration.

Confidence in your experiments

No worrying! Do your experiments with confidence, knowing that your pipettes are delivering accurate volumes.

Instant, easy verification, anywhere

No waiting! Evaluate pipette accuracy on the spot in just seconds – no matter where you are. Why mess with a scale?

Checkit is fast and easy. And no worries about evaporation or air currents. Your measured drop is encased in a glass tube within seconds, so the values won’t change while you’re trying to read them.

No more precautionary calibration

No unnecessary downtime while your pipettes are out for calibration. And no wasted calibration fees. Use the Checkit and instantly know what needs to be done.


Uses

Instant checks anytime

Keep Checkits in the lab for instant confirmation of pipette accuracy. Especially useful if your pipette has been dropped, cleaned, or borrowed. Don’t take a chance when you’re setting up an experiment with precious samples or expensive reagents.

Confirm accuracy between calibrations

Perform checks regularly to watch for performance drift between calibrations. A worn or damaged pipette can drift out of calibration quickly – get early warning before there’s a problem.

Train students and technicians

Good pipetting technique is an important part of laboratory success. Use the Checkit as a training tool to verify proper technique. The Checkit requires no special training and is easy to use even for novice lab members.

See the Pipette Checkit brochure.

Features

Features

  • Easy, user friendly operation
  • Easy to read markings indicate volumetric accuracy
  • ISO 8655 calibration standards indication
  • Elegant design
  • Minimal evaporation
  • Made in the USA


Brief Intro to the CheckIt

Models

Operation

Using the Checkit

Each Pipette Checkit™ has three precision glass capillaries matched to the top, middle, and low end of the range of your pipette. Pipet the three diagnostic volumes into the matching sample wells, flip the wells into measurement position, and take your readings. The pipetted volumes are compared to easy-to-read graduations that tell you instantly whether the pipette needs adjustment.

checkit pipette evaluation verification step 1 fill it

Step 1: Draw dye into the pipette tip.

Place the Checkit on a level surface. Grasp the pull tab on the reservoir seal and peel it off to expose the measurement fluid. Fit your pipette with an appropriate pipette tip (not a filter or gel-loading tip). Set your pipette volume. Then draw the dye (measurement fluid) from the reservoir into the tip.

checkit pipette evaluation verification step 2 dispense it

Step 2: Dispense dye into a well.

Pipet the liquid into the first well of the Checkit.

checkit pipette evaluation verification step 3 flip it

Step 3: Flip up the well tab.

Use your finger tip to rotate the well to the vertical position. The droplet will automatically be drawn into the measurement capillary.

checkit pipette evaluation verification step 4 check it

Step 4: Checkit!

Use the scale printed on the Checkit cartridge to determine the actual dispensed volume. Then repeat for the middle (50% of largest volume) and bottom (10% of largest volume) of the range of your pipette.

Note: Always wear personal protective equipment (PPE) including goggles.

Resources

Calibration vs. verification

The Pipette Checkit™ is a tool to identify potential volume delivery errors for pipettes. It is not a calibration tool. If your institution requires pipette calibration on a specific schedule, you should adhere to this schedule and use the Checkit as an extra verification step between scheduled calibrations.

Expected performance at different volumes

Piston pipettes are most accurate at or near their nominal volume (the maximum volume the pipette can deliver). When a pipette is used near the bottom of its range, the measured volumes will vary more, and are also more strongly affected by slight variations in pipetting style or environmental factors like humidity. The markings on the Checkit reflect this variation.

A note on environment: One advantage of the Checkit is that it can be used in the same room in which the experiment will be conducted. This can help identify variation in delivered volume due to pressure, humidity, or temperature differences between the calibration environment and the experimental environment. Because the Checkit directly measures delivered volume rather than relying on gravimetric analysis, there is also no need to use correction calculations that account for these factors.

Accuracy and precision

Most pipette manufacturers provide specifications for both the accuracy and precision of their pipettes. Briefly, accuracy describes how close the mean of delivered volumes is to the stated volume, and precision describes the distribution of the volumes around the mean. If you choose to compare your Checkit readings to the manufacturer’s specifications, remember to account for both accuracy and precision when determining what range is acceptable.

Note that ISO 8655 standards differ from the manufacturer’s standards. If your pipette has been recalibrated since purchase, you should check with your calibration service to determine which standards were used, and consider comparing your Checkit readings to that standard rather than to the manufacturer’s standard.

Useful links and resources

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